A Novel Role for PA28γ-Proteasome in Nuclear Speckle Organization and SR Protein Trafficking

Baldin, Véronique; Militello, Muriel; Thomas, Y.; Doucet, Christine; Fic, Weronika; Boireau, Stéphanie; Jariel-Encontre, Isabelle; Piechaczyk, Marc; Bertrand, Édouard; Tazi, Jamal; Coux, Olivier

doi:10.1091/mbc.e07-07-0637

Cited by 65 publications

(60 citation statements)

References 55 publications

(76 reference statements)

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“…Immunoprecipitations were performed as previously described. 21 Bead pellets were either boiled in SDS sample buffer, separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblot analysis as described 63 or subjected to histone-H1 kinase assay as described.…”

Section: Discussionmentioning

confidence: 99%

βTrCP-dependent degradation of CDC25B phosphatase at the metaphase-anaphase transition is a pre-requisite for correct mitotic exit

Thomas¹,

Coux²,

Baldin³

2010

Cell Cycle

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Section: Discussionmentioning

confidence: 99%

βTrCP-dependent degradation of CDC25B phosphatase at the metaphase-anaphase transition is a pre-requisite for correct mitotic exit

Thomas¹,

Coux²,

Baldin³

2010

Cell Cycle

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“…Furthermore, a few cyclin A2 foci colocalised with active proteasomes, which were identified by the rapid digestion of microinjected DQ ovalbumin (Fig. 2B) (Baldin et al, 2008;Rockel et al, 2005). Cdk binding is required for the correct timing of cyclin A2 degradation (den Elzen and Pines, 2001), and Cdks are likely to be associated with cyclins when the latter are degraded by the proteasome (Nishiyama et al, 2000).…”

Section: Dynamics Of Cyclin-a2-rich Focimentioning

confidence: 98%

High resolution live cell imaging reveals novel cyclin A2 degradation foci involving autophagy

Loukil

Zonca

Rebouissou

et al. 2014

Journal of Cell Science

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Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis relies on the ubiquitin-proteasome system (UPS). Using high-resolution microscopic imaging, we find that cyclin A2 persists beyond metaphase. Indeed, we identify a novel cyclin-A2-containing compartment that forms dynamic foci. Fö rster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) analyses show that cyclin A2 ubiquitylation takes place predominantly in these foci before spreading throughout the cell. Moreover, inhibition of autophagy in proliferating cells induces the stabilisation of a subset of cyclin A2, whereas induction of autophagy accelerates the degradation of cyclin A2, thus showing that autophagy is a novel regulator of cyclin A2 degradation.

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“…Decreased levels of p53 allow cells to pass through the G1/S checkpoint during cell cycle progression. Other studies have shown that REGg may play a key role in chromosomal stability during mitosis [43] and in the organization of nuclear speckles [44]. Furthermore, there was a report implicating a potential function of REGg in regulating the activity of G protein-coupled receptors through binding with the C-terminus of thromboxane A 2 receptor [45].…”

Section: Biological Functions Of Reggmentioning

confidence: 99%

REGγ, a proteasome activator and beyond?

Mao

Liu

Xiao

et al. 2008

Cell. Mol. Life Sci.

138

132

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REGgamma, a member of the 11S proteasome activators, has been shown to bind and activate the 20S proteasome to promote proteasome-dependent degradation of important regulatory proteins, such as SRC-3 and cyclin-dependent kinase inhibitors p21, p16, and p19, in a ubiquitin- and ATP-independent manner. Furthermore, REGgamma has been shown to facilitate the turnover of tumor suppressor p53 by promoting MDM2-mediated p53 ubiquitination. The discovery that REGgamma regulates cell-cycle regulators is consistent with previous studies where REGgamma-deficient mice have shown retardation in body growth, decreased cell proliferation and increased apoptosis, indicating a potential role of REGgamma in cancer development. Additionally, REGgamma's ability to promote viral protein degradation suggests its involvement in viral pathogenesis. This review presents an overview of the function of REGgamma, a summary of the current literature, and insight into the possible biological function of REGgamma relating to cancer, viral pathogenesis, and other diseases.

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A Novel Role for PA28γ-Proteasome in Nuclear Speckle Organization and SR Protein Trafficking

Cited by 65 publications

References 55 publications

βTrCP-dependent degradation of CDC25B phosphatase at the metaphase-anaphase transition is a pre-requisite for correct mitotic exit

βTrCP-dependent degradation of CDC25B phosphatase at the metaphase-anaphase transition is a pre-requisite for correct mitotic exit

High resolution live cell imaging reveals novel cyclin A2 degradation foci involving autophagy

REGγ, a proteasome activator and beyond?

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