A novel role for the glutamate decarboxylase system in Listeria monocytogenes; protection against oxidative stress

Abstract

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A multi-omics analysis strategy reveals the molecular mechanism of the inhibition of Escherichia coli O157:H7 by anthocyanins from Aronia melanocarpa and its application

“…It has been found that glutamic acid plays an active role in promoting the TCA cycle in the extensive metabolism process of bacterial cells. 31,32 Similarly, in this study, we found that the proteins (GdhA, GltB, GltD, AegA, GadA, and GadB) related to glutamate metabolism were significantly up-regulated. In addition, we noticed that the expression levels of proteins (AspA, AsnA, and AsnB) involved in aspartic acid metabolism were also up-regulated.…”

Reversion of antibiotic resistance in multidrug-resistant pathogens using non-antibiotic pharmaceutical benzydamine

“…These data were in agreement with the previous observations that the antibacterial activity of benzydamine was strengthened in the alkaline conditions and the genes associated with acid resistance were downregulated. Recently, several studies reported that GAD systems, which were downregulated in the combination group, play a critical role in protecting bacteria against oxidative stress 35 , 36 , we hypothesized that the potentiation of benzydamine to antibiotics may also correlate to enhanced oxidative damage. Thus, we tested the generation of reactive oxygen species (ROS) 37 in E. coli B2 treated by either benzydamine or in combination with doxycycline.…”

Investigation of memory-enhancing effects of Streptococcus thermophilus EG007 in mice and elucidating molecular and metagenomic characteristics using nanopore sequencing

“…It implies that an ancestral strain of EG007 may have originally acquired the operon by HGT from other GABA-producing microorganisms. According to previous findings, it has been reported that strains from L. monocytogenes , L. lactis , E. faecium were capable of producing GABA 72 – 74 . Furthermore, since microorganisms are able to raise the cytoplasm and extracellular pH by exporting the produced GABA, it is speculated that having the GAD system can be also beneficial for the microbe itself, by allowing them to survive better under acidic conditions 75 .…”