A Novel Role of Globular Adiponectin in Treatment with HFD/STZ Induced T2DM Combined with NAFLD Rats

Abstract

Aims. To evaluate the effects of globular adiponectin (gAd) on treatment of type 2 diabetic rats combined with NAFLD. Materials and Methods. Twenty-one male wistar rats were fed with normal diet (7 rats) or high fat diet (HFD) (14 rats) for 4 weeks, and then HFD-fed rats were injected with streptozotocin (STZ) to induce type 2 diabetes mellitus (T2DM). Half of T2DM rats were randomly injected with gAd intraperitoneally for 7 days. The expressions of adiponectin receptors (adipoR1/R2) in liver and skeletal muscle tissues were detected through western blotting or RT-qPCR, respectively. Results. Globular adiponectin alleviated the hepatic steatosis and increased insulin secretion. In liver, both the protein and mRNA expressions of adipoR2 in T2DM group decreased (P < 0.05, resp.) in contrast to NC group and increased (P < 0.05 and P < 0.001, resp.) after gAd treatment. But the protein and mRNA expressions of adipoR1 increased (P < 0.05, resp.) in T2DM group and no change was found in the gAd-treated group. In skeletal muscle, the protein and mRNA expressions of adipoR1 and adipoR2 were upregulated in T2DM group and were downregulated after gAd treatment. Conclusions. Globular adiponectin could ameliorate the hepatic steatosis and vary the expressions of adiponectin receptors in liver and skeletal muscle by stimulating insulin secretion.

Alleviative effect of Ruellia tuberosa L. on NAFLD and hepatic lipid accumulation via modulating hepatic de novo lipogenesis in high‐fat diet plus streptozotocin‐induced diabetic rats

“…HFD/STZ‐induced T2DM rats were carried out by the method of Ma et al. (2014) with slight modifications. After 1‐week adaptation, they were fed an HFD (60% calories from fat) for 4 weeks.…”

“…The rats were maintained under standard laboratory conditions at a temperature of 23 ± 1°C and a 12-hr light/12-hr dark cycle, with free access to food and water for the duration of the study. HFD/STZ-induced T2DM rats were carried out by the method of Ma et al (2014) with slight modifications. After 1-week adaptation, they were fed an HFD (60% calories from fat) for 4 weeks.…”

Type 2 Diabetes Mellitus Non-genetic Rhesus Monkey Model Induced by High Fat and High Sucrose Diet

“…The test monkeys were housed in single cage (80 × 80 × 100 cm) in experimental animal house with 3 meals, free to drink pure water and sufficient illuminance (12 h/day), temperature (20 °) and moisture (40 %). The animals were in the T2DM group injected with STZ at a dosage administration of 35 mg per kg of body weight by intraperitoneal injection as soon as hyperlipidemia and hyperinsulinemia symptom appeared in both of high-sucrose/high-fat group and T2DM group [20][21][22].…”

Fluoxetine regulates glucose and lipid metabolism via the PI3K‑AKT signaling pathway in diabetic rats

“…In addition, a meta-analysis based on five large-scale clinical trials revealed that the levels of FBG and TG were significantly reduced in patients with T2DM following treatment with fluoxetine hydrochloride compared with a placebo ( 9 , 10 ); however, the effects of fluoxetine on lipid metabolism and the underlying mechanisms remain unknown. In the present study, STZ-induced T2DM rats were used to mimic the metabolic characteristics of T2DM as reported previously ( 30 ); the effects of fluoxetine on glucose and lipid metabolism were investigated ( 31 ). Additionally, the levels of FBG and random glucose were significantly decreased in STZ-induced T2DM rats following treatment with fluoxetine hydrochloride in the present study, indicating that fluoxetine may reduce hyperglycemia and increase insulin sensitivity; the molecular mechanisms may be associated with muscle glycogen synthesis ( 32 ).…”