A novel ROS-Related chemiluminescent semiconducting polymer nanoplatform for acute pancreatitis early diagnosis and severity assessment

Abstract

Acute pancreatitis (AP) is a common and potentially life-threatening inflammatory disease of the pancreas. Reactive oxygen species (ROS) play a key role in the occurrence and development of AP. With increasing ROS levels, the degree of oxidative stress and the severity of AP increase. However, diagnosing AP still has many drawbacks, including difficulties with early diagnosis and undesirable sensitivity and accuracy. Herein, we synthesized a semiconducting polymer nanoplatform (SPN) that can emit ROS-correlated chemiluminescence (CL) signals. The CL intensity increased in solution after optimization of the SPN. The biosafety of the SPN was verified in vitro and in vivo. The mechanism and sensitivity of the SPN for AP early diagnosis and severity assessment were evaluated in three groups of mice using CL intensity, serum marker evaluations and hematoxylin and eosin staining assessments. The synthetic SPN can be sensitively combined with different concentrations of ROS to produce different degrees of high-intensity CL in vitro and in vivo. Notably, the SPN shows an excellent correlation between CL intensity and AP severity. This nanoplatform represents a superior method to assess the severity of AP accurately and sensitively according to ROS related chemiluminescence signals. This research overcomes the shortcomings of AP diagnosis in clinical practice and provides a novel method for the clinical diagnosis of pancreatitis in the future.

Ca/Fe-Based Nanozymes Relieve Severe Acute Pancreatitis by Ferroptosis Regulation and Reactive Oxygen Species Scavenging

“…Acute pancreatitis (AP) is a frequent acute abdomen in gastroenterology, which has the characteristics of acute onset, severe illness, and rapid change. − Around 20–30% of AP patients will develop serious diseases to severe acute pancreatitis (SAP) and have a higher mortality rate with a short period of time. , In SAP, the activation of pancreatic enzymes is often accompanied by pancreatic digestion, swelling, bleeding, and necrosis . Unfortunately, SAP could eventually cause damage to multiple organ functions in the body, especially influencing the lungs, kidneys, and liver. , At present, the treatment of SAP is mainly through targeted management of generalized complications. , Although treatment options for SAP have been extensively studied, there has been no significant improvement in clinical outcomes for SAP-related patients. Given that there are no clinically approved definitive medicines to treat SAP, the development of a novel and efficient intervention strategy for SAP treatment is an urgent demand …”

“…7,8 At present, the treatment of SAP is mainly through targeted management of generalized complications. 6,9 Although treatment options for SAP have been extensively studied, there has been no significant improvement in clinical outcomes for SAPrelated patients. Given that there are no clinically approved definitive medicines to treat SAP, the development of a novel and efficient intervention strategy for SAP treatment is an urgent demand.…”