A novel route of follicle‐stimulating hormone administration with a split‐single ischiorectal fossa in Thai‐Holstein crossbred superovulation programs under heat stress conditions

Ratsiri, Thanaporn; Ratchamak, Ruthaiporn; Chumchai, Rujira; Boonkum, Wuttigrai; Vongpralub, Thevin; Chankitisakul, Vibuntita

doi:10.1111/asj.13574

Cited by 6 publications

(7 citation statements)

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“…However, multiple injections of FSH are required due to its short half-life, which is time-consuming and may also be stressful for the cows (Sugano and Shinogi, 1999;Chasombat et al, 2013). Many previous studies proposed solving this issue by administering FSH dissolved in various agents that provide a slow release, such as polyvinylpyrrolidone (PVP) (Yamamoto et al, 1994;Takedomi et al, 1995;Ratsiri et al, 2021) and aluminium hydroxide gel (AH-gel) in cattle (Kimura, 2016). However, those diluting agents make the solution viscous and difficult to prepare well with FSH.…”

Section: Introductionmentioning

confidence: 99%

Ovarian responses and FSH profiles at superovulation with a single epidural administration of gonadotropin in the Thai-Holstein crossbreed

et al. 2021

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The conventional method of ovarian superstimulation requires multiple injections of gonadotropins which is time-consuming and may be stressful for the cows. This study was designed to determine whether a single epidural injection of FSH (EI group) would induce the superovulatory response in the Thai-Holstein crossbreed and evaluate FSH plasma hormone concentrations. Eight cows (replication = 3; n=24) were assigned to one of 2 treatments in switch back design. Control group (n=12): cows were received 400 mg FSH twice daily by intramuscularly for 4 days (80, 80, 60, 60, 40, 40, 20 and 20 mg), EI group (n=12): cows were received 400 mg FSH by single epidural injection. Data were collected in term of ovarian follicle responses, superovulatory responses, ova/embryo collection. FSH concentrations were examined using ELISA. The total follicular responses during oestrus were not different between treatments; however, the large follicles were less frequent (P < 0.01) while the medium follicle sizes were higher (P < 0.05) in the EI group. The plasma concentration of FSH in EI was dramatically increased within 2 hours before decreasing sharply thereafter (P < 0.01) and did not remain above baseline after 10 hours of administration. The embryo quality was better in the control than the EI groups (P < 0.05). Interestingly, the number of ovulation cysts in the EI group was 50%. The ovarian responses and embryo quality in the cows with cysts were worse compared with the non-cyst groups (P < 0.05). In conclusion, alternative protocols decreased the superovulatory response and increased poor embryo quality in Thai-Holstein crossbred. Also, the incidence of ovarian follicular cysts is higher in the EI group.

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Section: Introductionmentioning

confidence: 99%

Ovarian responses and FSH profiles at superovulation with a single epidural administration of gonadotropin in the Thai-Holstein crossbreed

et al. 2021

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“…Superovulation is the process of having the cows produce more follicles than the average count in each estrus by gonadotropin hormone and increasing the number of embryos. However, our previous study ( 25 ) reported that a high THI over 80–85 significantly decreased superovulatory efficiency by decreasing ovulation, fertilization, and transferable embryos response in Thai-Holstein crossbreeds. Meanwhile, the degenerated embryos significantly increased.…”

Section: Discussionmentioning

confidence: 86%

“…Figure 1 shows the traditional superovulation protocols that induce multiple gonadotropin treatments described previously ( 25 , 26 ). Briefly, on a random day of the estrus cycle (Day 0), estrus was synchronized using a CIDR-B device (Eazi-Breed CIDR-B®, Zoetis Animal Health, Kalamazoo, MI, USA) and an intramuscular (IM) injection of 5 mg estradiol-17β plus 50 mg progesterone (SRC Animal Health, Pak Chong, Nakhon Ratchasima, Thailand).…”

Section: Methodsmentioning

confidence: 99%

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The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions

et al. 2022

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The effect of heat stress with melatonin treatment on the superovulatory responses and embryo characteristics in Thai-Holstein crossbreeds under heat stress conditions was examined. Six non-lactating cows (replication = 4; n = 24) were assigned to one of 2 treatments in double cross-over design. All cows were superstimulated with traditional treatment. Melatonin group (n = 12): cows received intramuscularly injection 18 mg/50 kg. simultaneously with GnRH injection, while those in the control group (n = 12) received none. Bloods samples were taken to determine lipid peroxidation (MDA) and the activity of the antioxidant enzymes (superoxide dismutase; SOD). The experiment was conducted from April to September, which determined severe heat stress (the mean temperature-humidity index above 77). The results revealed that numbers of large follicles and corpora lutea were higher in the melatonin group than in the control group (p < 0.01). Numbers of recovered ova/embryos, fertilized ova, and transferable embryos were higher in the melatonin group (p < 0.01); meanwhile, more degenerated embryos were found in the control group (p < 0.01). Increased activity of the antioxidant enzymes SOD after melatonin administration decreased MDA levels (p < 0.05). In summary, melatonin administration benefited the ovarian response and embryo quality in superstimulated Thai-Holstein crossbreed affected by heat stress.

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“…Cows received 0.01 mg of GnRH (Receptal ® , MSD, Unterschleissheim, Germany) by IM to induce ovulation in the evening of Day 8 and were artificially inseminated 12 and 24 h later. Ova/embryos were collected 7 days after insemination (Day 16) using a nonsurgical technique to flush the uterine horns as described by Ratsiri et al [19], evaluated and classified for quality according to the criteria of Lindner and Wright [20]. The numbers of ova and embryos were recorded.…”

Section: Superovulation Treatment and Embryo Recoverymentioning

confidence: 99%

Relationship of the Temperature-Humidity Index (THI) with Ovarian Responses and Embryo Production in Superovulated Thai-Holstein Crossbreds under Tropical Climate Conditions

Ratchamak

Ratsiri

Chumchai

et al. 2021

Veterinary Sciences

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Heat stress strongly negatively affects reproductive traits in dairy cattle. The purpose of the present study was to investigate the effect of heat stress in superstimulated Thai-Holstein crossbreds under tropical climate conditions. Data included 75 records from 12 superovulated Thai-Holstein crossbreds between 2018 and 2020. Cows were superstimulated with conventional treatment. The mean temperature-humidity index (THI) was evaluated for three data collection periods: during the 9, 21 and 42-day periods before the insemination day to determine the period in which THI mostly affected superstimulation responses. The THI levels/thresholds were determined and interpreted together with the superovulatory response. A significantly negative correlation was obtained for the THI during the period 9 days before insemination. Negative effects on the number of large follicles and corpora lutea began at a THI of 72 and were apparently severe after a THI of 77, similar to the ovulation rate, fertilized ova and transferable embryos (p < 0.05). Meanwhile, more degenerated embryos were found with THI values (p < 0.05). The superovulatory response in Thai-Holstein crossbreds under tropical climate conditions is highly affected by heat stress starting at a THI of 72 and becomes more severe at a THI higher than 77.

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A novel route of follicle‐stimulating hormone administration with a split‐single ischiorectal fossa in Thai‐Holstein crossbred superovulation programs under heat stress conditions

Cited by 6 publications

References 53 publications

Ovarian responses and FSH profiles at superovulation with a single epidural administration of gonadotropin in the Thai-Holstein crossbreed

Ovarian responses and FSH profiles at superovulation with a single epidural administration of gonadotropin in the Thai-Holstein crossbreed

The Melatonin Treatment Improves the Ovarian Responses After Superstimulation in Thai-Holstein Crossbreeds Under Heat Stress Conditions

Relationship of the Temperature-Humidity Index (THI) with Ovarian Responses and Embryo Production in Superovulated Thai-Holstein Crossbreds under Tropical Climate Conditions

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