A novel sandwich-type immunosensor for detection of carcino-embryonic antigen using silver hybrid multiwalled carbon nanotubes/manganese dioxide

Han, Jian; Li, Yueyun; Feng, Jinhui; Li, Mingdang; Wang, Ping; Chen, Zhiwei; Dong, Yunhui

doi:10.1016/j.jelechem.2017.01.021

Cited by 47 publications

(13 citation statements)

References 71 publications

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“…To further improve the immobilization performance, cyclodextrins can be modified on CNTs. Han et al (2017) prepared a novel sensitive sandwich-type non-enzymatic electrochemical immunosensor using cyclodextrin-modified MWCNTs. Synthesized silver nanoparticles-carbon nanotubes/manganese dioxide (Ag NPs-MWCNTs/MnO 2 ) were used as a label for Ab2 to achieve duplex amplification of the electrochemical signal.…”

Section: Introductionmentioning

confidence: 99%

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

Zhang

Peng

et al. 2021

Front. Chem.

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Gastric cancer is one of the most common malignant tumors, and early diagnosis will be of great significance to improve the survival quality and overall treatment outcome evaluation of patients. Nanoelectrochemical immunosensor is an emerging biosensor combining nanotechnology, electrochemical analysis method and immunological technology, which has simple operation, fast analysis speed, high sensitivity, and good selectivity. This mini-review summarized immunoassay techniques, nanotechnology and electrochemical sensing for the early detection of gastric cancer. In particular, we focus on the tension of carbon nanomaterials in this field, including the functionalized preparation of materials, signal enhancement and the construction of novel sensing interfaces. Currently, various tumor markers are being developed, but the more recognized gastric cancer tumor markers are carcinoembryonic antigen (CEA), carbohydrate antigen (CA), CD44V9, miRNAs, and programmed death ligand 1. Among them, the electrochemical immunosensor allows the detection of CEA, CA, and miRNAs. The mini-review focused on the development of using carbon based materials, especially carbon nanotubes and graphene for immunosensor fabrication and gastric cancer markers detection.

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Section: Introductionmentioning

confidence: 99%

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

Zhang

Peng

et al. 2021

Front. Chem.

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“…CEA is one of the most widely used tumor markers associated with colorectal cancer, pancreatic cancer, gastric cancer, and lung cancer [6]. Generally, the normal serum level of CEA in humans should be less than 5 ng/mL which is the cutoff value for the indication of cancer [7,8]. In addition, changes in serum CEA levels in patients with colorectal cancer can be used to detect early recurrence after surgery [7].…”

Section: Introductionmentioning

confidence: 99%

A Graphene-PEDOT:PSS Modified Paper-Based Aptasensor for Electrochemical Impedance Spectroscopy Detection of Tumor Marker

Yen

Chao

Yeh

2020

Sensors

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A graphene and poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) modified conductive paper-based electrochemical impedance spectroscopy (EIS) aptasensor has been successfully fabricated by a simple and continuous coating process. A graphene/PEDOT:PSS modified paper electrode forms the nanocomposite providing a conductive and sensitive substrate for further aptamer functionalization of the biosensor. This low-cost paper-based aptasensor exhibits its sensitivity to carcinoembryonic antigens (CEA) in standard buffer solutions and human serum samples in a linear range of 0.77–14 ng·mL−1. The limit of detection (LOD) is found to be 0.45 ng·mL−1 and 1.06 ng·mL−1 for CEA in both samples, separately. This aptamer-based sensing device was also evaluated and received a good correlation with the immunoassay detection method. The proposed paper-based aptasensor has demonstrated its potential as a rapid simple point-of-care analytical platform for early cancer diagnosis in less developed areas where manufacturing facilities, analytical instruments, and trained specialists are limited.

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“…Carcinoembryonic antigen (CEA) is often in connection with colorectal carcinomas . The CEA level in serum is found to be extraodinarily high in people with a malignant tumor . Therefore, CEA is a very important tumor marker for cancer diagnosis and monitoring .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor of Carcinoembryonic Antigen Based on Concanavalin A-Functionalized Magnetic Copper Silicate Carbon Microtubes and Gold-Nanocluster-Assisted Signal Amplification

Zheng

Wang

Song

et al. 2020

ACS Appl. Nano Mater.

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An ultrasensitive electrochemical sensor was developed for the quantitative determination of carcinoembryonic antigen (CEA), which is often utilized as a tumor marker and a prognostic indicator. In the paper, first, copper silicate integrated with nitrogen-doped magnetic carbon microtubes (NCMTs@ Fe 3 O 4 @Cusilicate) was synthesized and employed to enrich concanavalin A (ConA), which can be used as an affinity probe to selectively capture CEA. Through chemical chelation between a copper ion and Con A, the combination of Cusilicate/ConA was greatly improved. Furthermore, gold nanoclusters (AuNCs) immobilized with aptamers were provided for signal amplification. Thus, in the presence of the target protein, CEA, in a sandwich manner, was formed, and AuNCs were gathered on the electrode through a magnetic field. Electrochemical oxidation of AuNCs to AuCl 4 − was performed, and immediately differential pulse voltammetry was performed, resulting in an electrochemical response because of the reduction of AuCl 4 − . On the basis of these merits, the constructed sensing system provided a ultrasensitive detection scheme for CEA with a linear range from 0.03 to 6.00 ng•mL −1 and a low detection limit of 5.38 pg•mL −1 . In addition, the proposed strategy was employed to detect CEA in serum, which showed excellent correlations with standard immunoassay. The excellent performance indicated that the proposed sensor has significant potential for clinical diagnosis.

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A novel sandwich-type immunosensor for detection of carcino-embryonic antigen using silver hybrid multiwalled carbon nanotubes/manganese dioxide

Cited by 47 publications

References 71 publications

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

Carbon Material Based Electrochemical Immunosensor for Gastric Cancer Markers Detection

A Graphene-PEDOT:PSS Modified Paper-Based Aptasensor for Electrochemical Impedance Spectroscopy Detection of Tumor Marker

Electrochemical Aptasensor of Carcinoembryonic Antigen Based on Concanavalin A-Functionalized Magnetic Copper Silicate Carbon Microtubes and Gold-Nanocluster-Assisted Signal Amplification

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