A novel HLA‐C allele, HLA‐C*03:614, identified by next‐generation sequencing in a Chinese individual

Abstract

HLA‐C*03:614 has one nucleotide change compared with HLA‐C*03:02:02:01 in exon 7, codon 336.

The novel HLA‐C*03:94:02 allele identified by next‐generation sequencing in a Chinese individual

“…Initially, we applied the polymerase chain reactionsequence specific oligonucleotide (PCR-SSO) method to genotype the HLA-A, -B, -C, -DQB1, and -DRB1 genes of this sample according to our previous report. 3,4 The results indicate that the HLA-C genotype of this sample did not match any known combination of HLA-C alleles. Therefore, polymerase chain reaction sequence-based typing (TBG Biotechnology Corp., Xiamen, China) was used to characterize the allele furtherly.…”

“…Initially, we applied the polymerase chain reaction‐sequence specific oligonucleotide (PCR‐SSO) method to genotype the HLA‐A, ‐B, ‐C, ‐DQB1, and ‐DRB1 genes of this sample according to our previous report 3,4 . The results indicate that the HLA‐C genotype of this sample did not match any known combination of HLA‐C alleles.…”

“…We amplified the HLA‐C*03 and HLA‐C*06 alleles separately with group specific primers. The sequence reaction products were processed with an ABI 3130XL DNA Analyzer (Applied Biosystems, Foster City, USA) and analyzed with AccuType software V1.5 (TBG Biotechnology Corp., Xiamen, China) 3,4 . The sequencing results showed that the HLA‐C alleles of the donor were HLA‐C*06:02 and a novel allele, now named HLA‐C*03:94:02 .…”

“…The sequence reaction products were processed with an ABI 3130XL DNA Analyzer (Applied Biosystems, Foster City, USA) and analyzed with AccuType software V1.5 (TBG Biotechnology Corp., Xiamen, China). 3,4 The sequencing results showed that the HLA-C alleles of the donor were HLA-C*06:02 and a novel allele, now named HLA-C*03:94:02. In the meantime, the sample was also genotyped by NGS method with AllType 11-Loci Amplification Kit according to our previous report.…”

“…In the meantime, the sample was also genotyped by NGS method with AllType 11-Loci Amplification Kit according to our previous report. 3,4 Compared with the HLA-C*03:94:01 allele, the novel allele HLA-C*03:94:02 shows one synonymous substitution from A to G at position 53 in exon 2 (Figure 1 The name C*03:94:02 has been officially assigned by the WHO Nomenclature Committee for Factors of the HLA System in September 2023. This follows the agreed policy that, subject to the conditions stated in the most recent Nomenclature Report, 5 names will be assigned to new sequences as they are identified.…”