A novel sensitive doxorubicin impedimetric immunosensor based on a specific monoclonal antibody–gold nanoaprticle–sol–gel modified electrode

Application of malachite green (MG) and leucomalachite green (LMG) in fish farm water causes an environmental problem. This study proposes for the first time a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining MG and LMG by a bovine serum albumin-decorated gold nanocluster (BSA-AuNC)/antibody composite film-based immunosensor. In order to improve the analytical performance, the glassy carbon electrode (GCE) was modified with 1, 4-phenylenediamine to form a stable layer, and then, BSA-AuNCs were covalently bound to the GCE. An adequate quantity of the polyclonal antibody of LMG was immobilized onto the surface of the BSA-AuNCs by the chemical reaction of EDC/NHS. The sensors can respond to the specific target based on specific covalent bonding. The experimental parameters, such as the pH, incubating concentration, and time, have been investigated and optimized. The calibration curve for LMG was linear in the range of 0.1~10.0 ng/mL with the limit of detection (LOD) 0.03 ng/mL. Furthermore, the sum of MG and LMG was detected in fish farm water by MG reduction. The recovery was between 89.7 % and 99.2 % in spiked samples. The EC sensor method was also compared with the ELISA method and validated by the LC-MS/MS method, which proves its great promise as a field instrument for the rapid monitoring of MG and LMG pollution. Graphical abstract 1, 4-Phenylenediamine and BSA-AuNC/antibody-decorated glassy carbon electrodes have been used for the impedimetric detection of the sum of malachite green and leucomalachite green via specific immuno-binding.

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“…6b, curve b), indicating that the influence of the nonspecific absorption could be ignored. [34]. Relative standard deviation (RSD) was 5.6 %.…”

Section: Calibration Curve Of the Immunosensormentioning

confidence: 96%

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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“…The fabricated immunosensor capability for DOX determination in spiked human serum and urine samples was examined, and the obtained results demonstrated that the immunosensor can be used as a good tool for DOX determination in biological samples. 87…”

Section: Modification Of Au-nps For Dau Deliverymentioning

confidence: 99%

<p>A review of small molecules and drug delivery applications using gold and iron nanoparticles</p>

Jahangirian¹,

Kalantari²,

Izadiyan³

et al. 2019

IJN

177

106

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Conventional cancer treatment techniques show several limitations including low or no specificity and consequently a low efficacy in discriminating between cancer cells and healthy cells. Recent nanotechnology developments have introduced smart and novel therapeutic nanomaterials that take advantage of various targeting approaches. The use of nanotechnology in medicine and, more specifically, drug delivery is set to spread even more rapidly than it has over the past two decades. Currently, many nanoparticles (NPs) are under investigation for drug delivery including those for cancer therapy. Targeted nanomaterials bind selectively to cancer cells and greatly affect them with only a minor effect on healthy cells. Gold nanoparticles (Au-NPs), specifically, have been identified as significant candidates for new cancer therapeutic modalities because of their biocompatibility, easy functionalization and fabrication, optical tunable characteristics, and chemophysical stability. In the last decade, there has been significant research on Au-NPs and their biomedical applications. Functionalized Au-NPs represent highly attractive and promising candidates for drug delivery, owing to their unique dimensions, tunable surface functionalities, and controllable drug release. Further, iron oxide NPs due to their "superparamagnetic" properties have been studied and have demonstrated successful employment in numerous applications. In targeted drug delivery systems, drug-loaded iron oxide NPs can accumulate at the tumor site with the aid of an external magnetic field. This can lead to incremental effectiveness in drug release to the tumor site and vanquish cancer cells without harming healthy cells. In order for the application of iron oxide NPs in the human body to be realized, they should be biodegradable and biocompatible to minimize toxicity. This review illustrates recent advances in the field drug and small molecule delivery such as fluorouracil, folic acid, doxorubicin, paclitaxel, and daunorubicin, specifically when using gold and iron oxide NPs as carriers of anticancer therapeutic agents.

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“…The relative charge transfer resistance (Rct) increases linearly with doxorubicin concentration in the ranges of 0.1-1.0 and 2.5-50.0 pg m L À1 , with a detection limit of 0.09 pg mL À1 . Application to spiked human serum and urine samples was reported [204]. …”

Section: Impedimetric Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Drug Analysis

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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A novel sensitive doxorubicin impedimetric immunosensor based on a specific monoclonal antibody–gold nanoaprticle–sol–gel modified electrode

Cited by 35 publications

References 22 publications

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

<p>A review of small molecules and drug delivery applications using gold and iron nanoparticles</p>

Electrochemical Biosensors for Drug Analysis

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