A novel serum protein of molecular weight 182 kDa: a molecular marker for an early detection of increased left ventricular mass in patients with cardiac hypertrophy

Insights into the Activity, Differential Expression, Mutual Regulation, and Functions of Matrix Metalloproteinases and A Disintegrin and Metalloproteinases in Hypertension and Cardiac Disease

“…In cancer it has been proposed that high levels of tissue inhibitor of metalloproteinases 2 cause MMP activation instead of inhibition by binding in a complex to MT1-MMP and LRP-1, signaling pro-growth pathways [6,182,183]. Similarly, LRP-1-mediated activation of the MAPK cascade can explain why high levels of the metalloproteinase inhibitor α 2 -macroglobulin, which binds to LRP-1, correlate with the development of cardiac hypertrophy [184,185,186,187]. Activation of LRP-1 by α 2 -macroglobulin addition causes hypertrophy of cardiomyocytes in culture [176].…”

Cardiac isoform of alpha-2 macroglobulin as a novel diagnostic marker for cardiac diseases

“…So far there is no single specific marker for evaluating all cardiac diseases. CA2M has shown to be involved in cardiac hypertrophy and the level of this protein could be an early marker identifying the stages of increase in left ventricular mass [3,4]. Direct injection and expression in vivo of full-length cDNA of CA2M was shown to induce cardiac hypertrophy in the rat heart [5].…”

“…In the present study we sought to specifically determine and clinically judge the diagnostic value of CA2M over the conventional information obtained in the evaluation of patients with cardiac and non-cardiac ailments by comparing with controls. Quantification of the CA2M serum protein CA2M from the blood serum of aorta-constricted rats was purified and raising of anti-rat CA2M antisera, immunocross reactivity between human CA2M and anti-rat CA2M antibody and western blot analysis were carried out as previously described [3][4][5][6]. The CA2M in the sera of normal human and patients with cardiac and noncardiac diseases were quantified by sandwich enzymelinked immunosorbent assay (ELISA) using anti-rat CA2M antibody raised in rabbit and mouse [4].…”

“…Quantification of the CA2M serum protein CA2M from the blood serum of aorta-constricted rats was purified and raising of anti-rat CA2M antisera, immunocross reactivity between human CA2M and anti-rat CA2M antibody and western blot analysis were carried out as previously described [3][4][5][6]. The CA2M in the sera of normal human and patients with cardiac and noncardiac diseases were quantified by sandwich enzymelinked immunosorbent assay (ELISA) using anti-rat CA2M antibody raised in rabbit and mouse [4]. The optimal concentration of antigens and antibody for coating was determined by checkerboard titration (BIORAD model 450 micro plate reader; Bio-Rad Laboratories, Hercules, California, USA).…”

Cardiac Isoform of Alpha 2 Macroglobulin and Its Reliability as a Cardiac Marker in HIV Patients

“…The Madurai Kamaraj University and Government Rajaji Hospital Ethical Committee have cleared the animal experiments and human samples used in this study and informed consent was obtained.The CA2M from the serum of aortic-constricted rats were purified and raising of anti-rat CA2M antisera, immuno-cross-reactivity between human CA2M and anti-rat CA2M antibody and western blot analysis was carried out as described in earlier studies[9,10]. The CA2M in the sera of HIV patients with cardiac and non-cardiac diseases was quantified by sandwich ELISA using anti-rat CA2M antibody raised in rabbit and mouse[11]. The optimal concentration of antigens and antibody for coating was determined by checkerboard titration (BIORAD model 450 micro plate reader: Bio-Rad Laboratories, Hercules, CA, USA).…”