A novel serum protein purification technique combined with surface-enhanced Raman spectroscopy for liver cancer detection

Tang, Shuzhen; Gao, Siqi; Xu, Jianshu; Zheng, Mengmeng; Huang, Yimei; Yu, Yun

doi:10.1080/00387010.2020.1867186

Cited by 6 publications

(1 citation statement)

References 35 publications

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“…The core reason for this is the low rate of early diagnosis; nearly 80% of the patients have entered the middle and late stages once found, losing the chance of radical surgical resection. The detection of tumor markers in serum is one of the methods for early diagnosis [2]. Alpha-fetoprotein (AFP), the most specific marker for liver cancer, is traditionally detected by many analytical techniques, including radioimmunoassay (RIA), chemiluminescence immunoassay (CLIA), enzyme-linked immunosorbent assay (ELISA), time-resolved fluorescence immunoassay (TRFIA), etc.…”

Section: Introductionmentioning

confidence: 99%

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum

Chen

Shi

2023

Diagnostics

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The rapid and accurate detection of alpha-fetoprotein (AFP) levels is of great significance for the diagnosis and later treatment evaluation of liver cancer. In this study, a novel integrated quartz crystal microbalance (QCM) immunosensor based on the design to contact liquids on one side only was developed for the label-free detection of AFP. Anti-AFP mouse monoclonal antibodies were immobilized onto the upper electrode surface of the pre-treatment crystals using the staphylococcus aureus protein A. The AFP antigens in human serum were captured by specific surface-coated antibodies, and testing was carried out by monitoring the corresponding changes in frequency. The concentration range of the antigen detected was 13–760 ng/mL. The frequency characteristics of the process of antibody immobilization were investigated in detail, and high reproducibility of AFP antibody immobilization was achieved (standard deviation (SD) = 2.2 Hz). The developed QCM measurement system demonstrated a short test time (only 30 min), good reproducibility (the biological activity can still maintain more than 90% of the first test till it is reused five times), and accuracy as good as the one achieved with the radioimmunoassay (the maximum relative deviation = 4.8%). The designed QCM test system can easily and quickly detect AFP concentrations up to 760 ng/mL, indicating that the developed QCM assay is likely to lead to an alternative approach in large-scale screening for liver cancer in the near future.

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Section: Introductionmentioning

confidence: 99%

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum

Chen

Shi

2023

Diagnostics

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Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Wang,

Fang,

Wang

et al. 2023

Advanced Science

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Early clinical diagnosis, effective intraoperative guidance, and an accurate prognosis can lead to timely and effective medical treatment. The current conventional clinical methods have several limitations. Therefore, there is a need to develop faster and more reliable clinical detection, treatment, and monitoring methods to enhance their clinical applications. Raman spectroscopy is noninvasive and provides highly specific information about the molecular structure and biochemical composition of analytes in a rapid and accurate manner. It has a wide range of applications in biomedicine, materials, and clinical settings. This review primarily focuses on the application of Raman spectroscopy in clinical medicine. The advantages and limitations of Raman spectroscopy over traditional clinical methods are discussed. In addition, the advantages of combining Raman spectroscopy with machine learning, nanoparticles, and probes are demonstrated, thereby extending its applicability to different clinical phases. Examples of the clinical applications of Raman spectroscopy over the last 3 years are also integrated. Finally, various prospective approaches based on Raman spectroscopy in clinical studies are surveyed, and current challenges are discussed.

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Raman scattering-based optical sensing of chronic liver diseases

Alkhuder

2023

Photodiagnosis and Photodynamic Therapy

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A novel serum protein purification technique combined with surface-enhanced Raman spectroscopy for liver cancer detection

Cited by 6 publications

References 35 publications

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum

Current Trends of Raman Spectroscopy in Clinic Settings: Opportunities and Challenges

Raman scattering-based optical sensing of chronic liver diseases

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