A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector

Gan, Ning; Xiong, Ping; Wang, Ji; Li, Tianhua; Hu, Futao; Cao, Yuting; Zheng, Lei

doi:10.1155/2013/482316

Cited by 19 publications

(12 citation statements)

References 22 publications

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“…The obtained detection limits were comparable with those obtained with QCM and ELISA technique in human plasma using doped magnetic core-shell [8].…”

Section: Introductionsupporting

confidence: 77%

“…The advantages of oriented immobilization of biologically active protein G are good steric accessibilities of active binding sites of the antibody and increased stability and sensitivity of the immunosensor [13]. The obtained results were better those obtained with QCM (Quartz Crystal Microbalance) and ELISA techniques in human plasma using doped magnetic core-shell [8].…”

Section: Spri Detection In Human Plasma From Patientmentioning

confidence: 76%

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Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Chammem¹,

Hafaid²,

Meilhac³

et al. 2014

JBNB

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In this work, we describe an approach of detecting biomarkers by Surface Plasmon Resonance imaging (SPRi) technique in real samples. Two C-Reactive Protein (CRP)-antibody immobilization methods were used: The first method was based on direct physisorption of CRP-antibody onto gold surface; the second one was based on oriented CRP-antibody with protein G intermediate layer.The two developed immunosensors were tested against CRP antigen in phosphate buffer saline solution with the SPRi technique. The response of the developed immunosensors was reproducible and stable. The detection limit of 10 pg•mL −1 and 50 pg•mL −1 CRP-antigen was observed with and without protein G respectively with this technique. Moreover, the developed SPRi immunosensor was used for CRP-antigen detection in human plasma. A detection limit of 5 ng•mL −1 and 10 ng•mL −1 was obtained with and without protein G respectively. These obtained results were compared to those obtained with QCM (Quartz Crystal Microbalance) and Enzyme-Linked Immunosorbent Assay (ELISA) techniques.

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“…The obtained detection limits were comparable with those obtained with QCM and ELISA technique in human plasma using doped magnetic core-shell [8].…”

Section: Introductionsupporting

confidence: 77%

Section: Spri Detection In Human Plasma From Patientmentioning

confidence: 76%

Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Chammem¹,

Hafaid²,

Meilhac³

et al. 2014

JBNB

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“…The linear range was 0.003-200 ng/mL and the detection limit was 1 pg/mL. 17 Compared to our new method demonstrated in this study, the authors used a more sophisticated procedure and required more chemicals. Their experiments also needed much longer time to finish.…”

Section: Resultsmentioning

confidence: 86%

Use of Magnetic Nanoparticles and a Microplate Reader with Fluorescence Detection to Detect C‐reactive Protein

Yang

Lin

et al. 2013

J Chinese Chemical Soc

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Two approaches based on magnetic nanoparticles (MNPs) have been compared to analyze C-reactive protein (CRP). Both the non-eluted and eluted MNP-1°Ab-CRP-2°Ab/FITC bioconjugates were measured by a microplate reader with fluorescence detection. The linear ranges for the non-elution and elution methods were 10-200 and 0.1-2.0 µg/mL, respectively. The concentration limits of detection for the nonelution and elution methods were 2.91 and 0.04 µg/mL, respectively. The non-elution method gave better precision and recovery than the elution method, and also showed comparable results to that of ELISA assay. The non-elution method is simple and only needs minute volumes of sample and buffer. There is no need to dissociate the fluorescence probes from the bioconjugates, and the fluorescence signals can be directly measured on the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates. Meanwhile, samples with high CRP concentrations are not necessarily to be diluted before analysis.

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“…Gan et al functionalized the surface of a QCM with a combination of Fe 3 O 4 and Au nanoparticles [ 104 ]. The Fe 3 O 4 magnetic nanoparticles were coated with SiO 2 , immersed in APTES to form amine groups (NH 2 ), and then coated with Au nanoparticles that were used to immobilize anti-CRP and HRP.…”

Section: Sensors For C-reactive Proteinmentioning

confidence: 99%

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

Salvo

Dini

Kirchhain

et al. 2017

Sensors

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Wound assessment is usually performed in hospitals or specialized labs. However, since patients spend most of their time at home, a remote real time wound monitoring would help providing a better care and improving the healing rate. This review describes the advances in sensors and biosensors for monitoring the concentration of C-reactive protein (CRP), temperature and pH in wounds. These three parameters can be used as qualitative biomarkers to assess the wound status and the effectiveness of therapy. CRP biosensors can be classified in: (a) field effect transistors, (b) optical immunosensors based on surface plasmon resonance, total internal reflection, fluorescence and chemiluminescence, (c) electrochemical sensors based on potentiometry, amperometry, and electrochemical impedance, and (d) piezoresistive sensors, such as quartz crystal microbalances and microcantilevers. The last section reports the most recent developments for wearable non-invasive temperature and pH sensors suitable for wound monitoring.

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A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector

Cited by 19 publications

References 22 publications

Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Use of Magnetic Nanoparticles and a Microplate Reader with Fluorescence Detection to Detect C‐reactive Protein

Sensors and Biosensors for C-Reactive Protein, Temperature and pH, and Their Applications for Monitoring Wound Healing: A Review

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