A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer’s disease blood biomarker

Karaboğa, Münteha Nur Sonuç; Sezgintürk, Mustafa Kemal

doi:10.1016/j.jpba.2018.03.016

Cited by 26 publications

(16 citation statements)

References 27 publications

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“…This sensor exhibited a bit lower affinity with a linear CRP concentration range from 21 to 6148 fg mL −1 and a comparable LOD of 0.34 fg mL −1 , achieving good storage stability (4.5% loss over 6 weeks). 519 5.5.2. Interleukin.…”

Section: Myoglobin (Myo): Cardiacmentioning

confidence: 99%

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Zhang

Zhu

et al. 2023

Chem. Rev.

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Whole blood, as one of the most significant biological fluids, provides critical information for health management and disease monitoring. Over the past 10 years, advances in nanotechnology, microfluidics, and biomarker research have spurred the development of powerful miniaturized diagnostic systems for whole blood testing toward the goal of disease monitoring and treatment. Among the techniques employed for whole-blood diagnostics, electrochemical biosensors, as known to be rapid, sensitive, capable of miniaturization, reagentless and washing free, become a class of emerging technology to achieve the target detection specifically and directly in complex media, e.g., whole blood or even in the living body. Here we are aiming to provide a comprehensive review to summarize advances over the past decade in the development of electrochemical sensors for whole blood analysis. Further, we address the remaining challenges and opportunities to integrate electrochemical sensing platforms.

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Section: Myoglobin (Myo): Cardiacmentioning

confidence: 99%

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Zhang

Zhu

et al. 2023

Chem. Rev.

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“…The proposed immunosensor was sensitive, with a detection limit of 0.455 fg mL −1 . Detection of CRP in human serum samples was measured by fabricated biosensor to determine the feasibility of the biosensing system for medical purposes [ 56 ]. Another miniaturized platform designated for ultrasensitive biosensor employs monoclonal amyloid-beta antibodies (mAb) as a biorecognition elements immobilized on a disc-shaped platinum/iridium (Pt/Ir) microelectrode surface.…”

Section: Electrochemical Biosensors For Ad Biomarkers Detectionmentioning

confidence: 99%

“…Nonetheless, the covalent binding between these molecules and the electrode surface is one of the most widely used. Just for examples, immobilization of antibodies on the glutaraldehyde (GA) layer [ 55 ] or on 3 cyanopropyltrimethoxysilane self-assembled monolayers [ 56 ] or also immobilization of thiolated antibodies on the gold surface [ 57 ] has been reported.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the field of biosensors has been growing, and the application of nanotechnology has developed as one of the biggest opportunities to achieve higher sensitivity for biosensors [ 58 ]. At the present time, in the construction of electrochemical biosensors for biomedical applications associated with AD biomarkers detection, the use of different types of nanomaterials like porous magnetic microspheres [ 59 ], nanotubes [ 60 ], graphene oxide [ 61 ], indium tin oxide [ 56 ] and metallic nanoparticles [ 62 ] has been reported in this review.…”

Section: Introductionmentioning

confidence: 99%

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Review—Recent Advancements in Electrochemical Biosensors for Alzheimer’s Disease Biomarkers Detection

Mikuła

2020

CMC

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Background: It is estimated that the average time between the diagnosis of Alzheimer’s disease (AD) and the patient’s death is 5-9 years. Therefore, both the initial phase of the disease and the preclinical state can be included in the critical period in disease diagnosis. Accordingly, a huge progress has recently been observed in biomarker research to identify risk factors for dementia in older people with normal cognitive functions and mild cognitive impairments. Methods: Electrochemical biosensors are excellent analytical tools that are used in the detection of AD biomarkers as they are easy to use, portable, and can do analysis in real time. Results: This review presents the analytical techniques currently used to determine AD biomarkers in terms of their advantages and disadvantages; the most important clinical biomarkers of AD and their role in the disease. All recently used biorecognition molecules in electrochemical biosensor development, i.e. receptor protein, antibodies, aptamers and nucleic acids are summarized for the first time. Novel electrochemical biosensors for AD biomarker detection, as ideal analytical platforms for point-of-care diagnostics, are also reviewed. Conclusions: The article focuses on various strategies of biosensor chemical surface modifications to immobilize biorecognition molecules, enabling specific, quantitative AD biomarker detection in synthetic and clinical samples. In addition, this is the first review that presents innovative single-platform systems for simultaneous detection of multiple biomarkers and other important AD-associated biological species based on electrochemical techniques. The importance of these platforms in disease diagnosis is discussed.

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“…The alkyl chain increases the stability of the single layer owing to van der Waals interactions. The terminal group provides chemical groups to bind biomolecules [33,34].…”

Section: Introductionmentioning

confidence: 99%

A sensitive and label-free electrochemical impedance immunosensor for CDH 22 biomarker detection based on organo-functional silane modified ITO electrode

Aydın

2019

Hacettepe Journal of Biology and Chemistry

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B u çalışmada, anti-CDH 22 antikoru ve CDH 22 antijeni arasındaki spesifik immüno-reaksiyona dayanan, kadherin benzeri protein (CDH 22) biyobelirtecinin tespiti için yeni bir elektrokimyasal etiketsiz immünosensör üretilmiştir. Geliştirilen immünosensör, anti-CDH 22 antikorlarının 3-siyanopropiltrimetoksisilan (CPTMS) ile modifiye edilmiş ITO substratı üzerinde immobilizasyonu ile oluşturulmuştur. Anti-CDH 22 antikorlarının CPTMS silanizasyon ajanına etkili bağlanması, morfolojik karakterizasyon (Taramalı Elektron Mikroskobu (SEM), Atomik Kuvvet Mikroskobu (AFM)) ve elektrokimyasal karakterizasyon (Elektrokimyasal İmpedans Spektroskopisi (EIS), Siklik Voltammetri (CV) kullanılarak incelendi. Optimum deney koşulları altında, ITO / CPTMS ile modifiye edilmiş elektrot, anti-CDH 22 antikor bağlanması için iyi bir bağlayıcıydır. Ek olarak, CPTMS modifiye elektrot CDH 22 antijen tespiti için etkili bir yüzey sunmuştur. İmmünosensör, geniş bir lineer tespit aralığı (0.03-3 pg/mL) ile düşük tespit limiti (9 fg/mL) ile sahipti. Ayrıca, iyi tekrarlanabilirlik, mükemmel tekrarlanabilirlik ve uzun depolama kararlılığına sahipti. Ek olarak, önerilen immünosensörün pratik uygulanabilirliği, insan serum numuneleri kullanılarak araştırıldı. İnsan serum numuneleri geri kazanım sonuçları (% 99.16-% 101.94) önerilen biyosensörün doğruluğunu göstermektedir. Sonuç olarak, CPTMS, biyosensör yapımı için umut verici bir platform olabilir ve bu önerilen immünosensör gerçek insan serum analizi için uygulanabilir. Anahtar Kelimeler Kadherin benzeri protein, elektrokimyasal impedans spektroskopisi, tek kullanımlık elektrot.

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A novel silanization agent based single used biosensing system: Detection of C-reactive protein as a potential Alzheimer’s disease blood biomarker

Cited by 26 publications

References 27 publications

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Review—Recent Advancements in Electrochemical Biosensors for Alzheimer’s Disease Biomarkers Detection

A sensitive and label-free electrochemical impedance immunosensor for CDH 22 biomarker detection based on organo-functional silane modified ITO electrode

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