A Novel, Simple Method for Detection of Caffeine in Urine as a Predictive Marker for Liver Diseases

Abstract

Drugs, chemicals, and endogenous substrates are all metabolized by hepatic cytochrome P450 (CYP) which plays a role in the aetiology of a variety of liver illnesses. Caffeine is completely metabolized by CYPs. Caffeine excretion in the urine is a sign of liver damage. The study aimed to develop and validate an inexpensive, simple, and rapid method for determining urinary caffeine as a diagnostic marker for liver diseases. Caffeine in urine sample underwent hydrolysis. Strip containing phosphomolybdic acid (PMA) was used to detect the hydrolysis product of caffeine in urine. The interaction of caffeine with PMA produced a blue color on the strip. The current method helps analyze caffeine in urine, providing a simple, rapid, and economic routine analysis.