A novel spectrofluorometric microassay for Streptococcus mutans adherence to hydroxylapatite

Gaines, Susan; James, Tharappel C.; Folan, Michael A.; Baird, Alan W.; O’Farrelly, Cliona

doi:10.1016/s0167-7012(03)00015-0

Cited by 32 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is possible that the same process is occurring in our adhesion assays. Other foods and beverages which are capable of inhibiting adhesion of cariogenic bacteria to S-HA include herbal extracts, cranberry juice and water-soluble protein fraction (WSPF) from hen-egg yolk, where 80, 90 and 61% inhibition of S. mutans adhesion to HA has been reported using 5, 250 g L -1 and 5 mg mL -1 of test substance, respectively [7,27,28].…”

Section: Assessment Of Anti-adhesive Effect Of Milk Componentsmentioning

confidence: 99%

“…Hydroxylapatite (HA) can be used as a suitable in vitro model of adherence to tooth surfaces. The HA model [5] is currently the most common in vitro system in use for the study of adhesion of oral bacteria such as S. mutans [6,7]. Phosphate-buffered saline coated HA (PBS-HA) was used in the majority of our adherence assays, however, some of our adhesion experiments were carried out in the presence of saliva for comparison purposes.…”

Section: Introductionmentioning

confidence: 99%

“…A fluorescence-based 96 well plate assay is one such approach that has been successfully employed by researchers such as Gaines et al [7]. Many other researchers use radiolabelled bacteria for the quantification of inhibition of adhesion of cariogenic bacteria to HA.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Halpin

O'Connor

McMahon

et al. 2008

Eur Food Res Technol

View full text Add to dashboard Cite

The aim of this study was to investigate the inhibitory effect of various dairy powders and milk constituents on the adhesion of a clinical isolate of Streptococcus mutans to hydroxylapatite (HA), an analogue of tooth enamel. Adhesion of a microorganism to a cell surface such as epithelial cells or tooth enamel is considered to be the first step in pathogenesis. Inhibiting this process may have therapeutic effects in vivo. The adherence assays were performed by incubating S. mutans with HA in the presence of each test material for 45 min, followed by centrifugal separation of the HA. Unbound bacteria were then quantified using a fluorescent dye. Sweet and Acid WPC80, buttermilk powder and cream powder were found to very effectively inhibit adherence of S. mutans to phosphate-buffered saline coated HA (PBS-HA). Sodium caseinate and the casein fractions a-,b-and j-casein were also found to show high levels of anti-adhesive activity. A selection of test materials were assessed using saliva-coated HA (S-HA), and similar trends were observed. The results suggest commercial dairy powders, and certain milk proteins, can inhibit adhesion of S. mutans to HA and may have potential to control dental caries.

show abstract

Section: Assessment Of Anti-adhesive Effect Of Milk Componentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Halpin

O'Connor

McMahon

et al. 2008

Eur Food Res Technol

View full text Add to dashboard Cite

show abstract

“…Oral care, with a focus on oral hygiene to reduce the dental plaque, can help decrease the incidence of pneumonia 10,11) . Among the microorganisms present in denture plaques, Candida albicans (C. albicans) and Streptococcus mutans (S. mutans) are well known as pathogens causing oral infections 2,[12][13][14][15][16] . The level of C. albicans increases in the oral cavity following the insertion of dentures, and can cause denture stomatitis, aspiration pneumonia and intestinal infections 2,[12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

“…The level of C. albicans increases in the oral cavity following the insertion of dentures, and can cause denture stomatitis, aspiration pneumonia and intestinal infections 2,[12][13][14] . S. mutans is a caries-causing oral Streptococci 15,16) , and is associated with inducing dental caries on the remaining teeth in the oral cavity of denture wearers.…”

Section: Introductionmentioning

confidence: 99%

An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models

et al. 2014

View full text Add to dashboard Cite

The purpose of this study was to perform a quantitative and qualitative evaluation of the biofilms formed on framework alloys in vitro. The biofilms formed by unfiltered fresh human saliva or Streptococcus mutans and/or Candida albicans on commercially pure titanium and gold-copper-platinum demonstrated higher amounts than other alloy and resin samples. In contrast, the silverpalladium-copper-gold showed a significantly decreased level of biofilm formation. Although the adhesion level of Streptococcus mutans on cobalt-chromium was high, that of Candida albicans was less extensive. A T-RFLP analysis and qualitative PCR of the microbes in the biofilms were performed. In a cluster analysis of all T-RFLP profiles, the cobalt-chromium pattern was integrated into one cluster. On qualitative PCR, the existence of microorganisms related to caries, preriodontitis and aspiration pneumonia was observed. Our results showed that the biofilm formation on each framework alloy was different in terms of both the quantity and quality.

show abstract

Investigation of Microbial Biofilm Structure by Laser Scanning Microscopy

Neu

Lawrence

2014

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

Microbial bioaggregates and biofilms are hydrated three-dimensional structures of cells and extracellular polymeric substances (EPS). Microbial communities associated with interfaces and the samples thereof may come from natural, technical, and medical habitats. For imaging such complex microbial communities confocal laser scanning microscopy (CLSM) is the method of choice. CLSM allows flexible mounting and noninvasive three-dimensional sectioning of hydrated, living, as well as fixed samples. For this purpose a broad range of objective lenses is available having different working distance and resolution. By means of CLSM the signals detected may originate from reflection, autofluorescence, reporter genes/fluorescence proteins, fluorochromes binding to specific targets, or other probes conjugated with fluorochromes. Recorded datasets can be used not only for visualization but also for semiquantitative analysis. As a result CLSM represents a very useful tool for imaging of microbiological samples in combination with other analytical techniques.

show abstract

A novel spectrofluorometric microassay for Streptococcus mutans adherence to hydroxylapatite

Cited by 32 publications

References 28 publications

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

Inhibition of adhesion of Streptococcus mutans to hydroxylapatite by commercial dairy powders and individual milk proteins

An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models

Investigation of Microbial Biofilm Structure by Laser Scanning Microscopy

Contact Info

Product

Resources

About