A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent

Abstract

A selective and new spectrophotometric method is described for determination of five 1,4-dihydropyridine drugs (1,4-DHP); namely nifedipine (NIF), nicardipine (NIC), nimodipine (NIM), felodipine (FEL) and amlodipine (AML). The method is based on a coupling reaction between the cited drugs and vanillin reagent in acidic condition. Under optimized conditions, the red coloured products were measured at 500 nm for NIF, NIC, NIM and FEL or at 479 nm for AML. Molar absorptivities were ranged from 0.575 × 10 4 -1.065 × 10 4 l·mol, Beer's law was obeyed at 5 -70 µg/mL concentration range and the limit of detection was ranged from 0.150 -1.500 µg/mL. The proposed method was successfully extended to pharmaceutical preparations tablets and capsules and comparison by Student's t-test and variance ratio F-test showed no significant difference.

Newfangled Quantitative Pharmacia (Isradipine) Stratagem: Quality by Design (Qbd) Taguchi Array via Response Surface (L25 Via CCD20) Methodology

“…The preferred drug (Isradipine standard) reference procured was as kindly gifted by Pharmaceuticals Ltd (India) and lambda maximum (λ max ; Figure 1) [24,39] was measured by double beam (Jasco, UV-630) spectrophotometer method [38] using one centi-meter quartz cells. All analytical grade solvents were and reagents were purchased from Lakshya Enterprises and Mercury Lab., New-Delhi, India.…”

“…Biologically, the selected model drug pine mechanism is bound L-type of Calcium Channel blocker (CCB s ) flux of cardiac and also smooth muscles with prominent specificity via affinity and inhibiting [3][4][5][6][7][8]. Literature, for CCB s plentiful techno and estimation methods were like Titrimetric [9,10], spectroscopic [11][12][13][14][15][16][17][18], Fluorimetric [19][20][21][22][23][24][25][26], electrochemical [27][28][29], Chromatographic methods [30][31][32][33][34][35][36][37][38] already reported [39][40][41][42]. Therefore, except a few anti-BP drugs till date there is no analytical quality design (QbD) stratagem.…”

Fruit/Vegetable–drug Interactions: Pharmacokinetic Assay With a Cyp3a4 Substrate

“…[15] A drug free plasma sample was used as blank. To 200μL aliquot of a plasma sample, 20μL methanol was added and volumes were made up to 1.5ml with 35.5 %v/v HCl.…”

Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs UsingN-Bromosuccinimide and Indigo Carmine Dye

“…Many analytical methods were developed for determination of 1,4-DHP drugs, namely, nifedipine (NIF), nicardipine (NIC), nimodipine (NIM), felodipine (FEL), and amlodipine (AML) (Figure 1), in their pharmaceutical formulations and in biological fluids, such as titrimetric methods [3,4], spectrometric methods (spectrophotometry [5][6][7][8][9][10][11][12][13] or spectrofluorimetry [12,[14][15][16][17][18][19][20]), electrochemical methods [21][22][23], liquid chromatographic methods [24][25][26][27][28], and gas chromatographic methods [29][30][31][32].…”