A Novel Standardized Cannabis sativa L. Extract and Its Constituent Cannabidiol Inhibit Human Polymorphonuclear Leukocyte Functions

Tagne, Alex Mabou; Marino, Franca; Legnaro, Massimiliano; Luini, Alessandra; Pacchetti, Barbara; Cosentino, Marco

doi:10.3390/ijms20081833

Cited by 24 publications

(30 citation statements)

References 48 publications

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“…Similarly, activation of the GPR55 receptor present on neutrophils leads to a reduction in the production of ROS, as well as degranulation [ 58 ]. On the other hand, in previous studies CBD has not shown an effect on neutrophil viability [ 18 ] and its effect on NETosis has not been analyzed. In the study presented here, our results show that CBD decreases rate of NETosis in neutrophils and also production of most of markers of NETosis.…”

Section: Discussionmentioning

confidence: 99%

“…The effects of CBD on neutrophils have not been sufficiently studied so far. CBD has recently been observed to inhibit neutrophil migration and their production of ROS, since preincubation of neutrophils with CBD leads to significantly lower ROS levels [ 18 ]. As mentioned above, cannabidiol works by activating specific receptors, in the case of immune cells mainly through CB2.…”

Section: Discussionmentioning

confidence: 99%

“…One potential candidate for therapeutic action seems to be cannabidiol (CBD)—a phytocannabinoid found in Cannabis Sativa L., which has no psychoactive effect, but is an anti-inflammatory and antioxidant compound, and its use has shown a positive effect on psoriatic skin cells [ 16 , 17 ]. Since it is known that CBD may reduce neutrophil activity and ROS production therein [ 18 , 19 ], its beneficial effect may be, at least in part, due to the weakening of the skin infiltrating neutrophils. By preventing the production of ROS, CBD may act as a NETosis inhibitor.…”

Section: Introductionmentioning

confidence: 99%

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Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients

Wójcik

Garley

Wroński³

et al. 2020

IJMS

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Psoriasis is associated with increased production of reactive oxygen species which leads to oxidative stress. As antioxidants can provide protection, the aim of this study was to evaluate the effects of cannabidiol (CBD) on neutrophil extracellular trap (NET) formation in psoriatic and healthy neutrophils. Important markers of NETosis were measured in healthy and psoriatic neutrophils after incubation with CBD, lipopolysaccharide (LPS), and LPS + CBD). The percentage of neutrophils undergoing NETosis and the level of NETosis markers (cfDNA, MPO, elastase) were higher in the neutrophils and blood plasma of psoriatic patients, compared to controls. After LPS treatment, all of the markers of NETosis, except elastase, and p47 and citrullinated histones, were increased in samples from healthy subjects and psoriasis patients. CBD reduced the concentrations of NETosis markers. This led to a reduction in NETosis, which was more pronounced in psoriatic neutrophils and neutrophils treated with LPS in both psoriatic and healthy participants. These results suggest that psoriatic patients neutrophils are at a higher risk of NETosis both in vitro and in vivo. CBD reduces NETosis, mainly in psoriatic neutrophils, possibly due to its antioxidant properties. The anti-NET properties of CBD suggest the positive effect of CBD in the treatment of autoimmune diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients

Wójcik

Garley

Wroński³

et al. 2020

IJMS

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“…14 Our DDG search on the Internet found more indications for CBD, including in the treatment of oral and genital herpes, HIV/AIDS, cold and flu, shingles, hepatitis C, Ebola, mononucleosis, progressive multifocal leukoencephalopathy, and West Nile and Zika fevers. Reportedly, the antiviral potential of CBD lies in the fact that it may affect both the innate and adaptive immune cells functions 29,30 and host inflammatory responses play a critical role in the pathogenesis of these viral diseases. However, apart from hepatitis C, there is a very real lack of scientific evidence supporting the other alleged antiviral properties of CBD.…”

Section: Discussionmentioning

confidence: 99%

Cannabidiol for Viral Diseases: Hype or Hope?

Tagne

Pacchetti²,

Sodergren

et al. 2020

Cannabis and Cannabinoid Research

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Background: The possibility of cannabidiol (CBD) to be used as an antiviral or to treat viral diseases has received limited attention so far, despite the growing number of claims that CBD could be used for the treatment of viral infection-related conditions. Aim and Methods: Therefore, we systematically retrieved and critically evaluated the scientific literature available on PubMed and the claims on the Internet, to assess the current state of knowledge on the use of CBD in viral diseases, and to provide suggestions for future research directions. Results: PubMed search referenced two original articles supporting the use of CBD for the treatment of hepatitis C and Kaposi sarcoma and one article reporting the ability of CBD to reduce neuroinflammation in a virusinduced animal model of multiple sclerosis. Internet search found 25 websites claiming more indications for CBD. Remarkably, those claims were provided mostly by commercial websites and were not supported by appropriate scientific references. Conclusion: Although preclinical studies suggest the potential effectiveness of CBD in viral diseases such as hepatitis C and Kaposi sarcoma, clinical evidence is still lacking. Anecdotal experiences of CBD use retrieved on the Internet, on the other side, lack any support from sound scientific evidence, although they might in some cases provide suggestions for conditions associated with viral infections that may deserve proper assessment in welldesigned clinical trials.

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“…Some of the anti-inflammatory effects of cannabis are likely through the suppression of PMN function. PMNs express the cannabinoid receptor (CB2) (176) and evidence suggests that cannabinoids suppress PMN function including cell migration, production of ROS and TNF-α production (177). In vivo, inhibition of the CB2 receptor suppressed PMN-mediated tissue damage in a mouse model of myocardial ischemia/reperfusion injury (178), and mice that lack CB2 expression exhibit exacerbated PMN recruitment during infection (179).…”

Section: Potential Therapeutic Pmn-specific Interventionsmentioning

confidence: 99%

The Neutrophil: Constant Defender and First Responder

et al. 2020

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The role of polymorphonuclear neutrophils (PMNs) in biology is often recognized during pathogenesis associated with PMN hyper-or hypo-functionality in various disease states. However, in the vast majority of cases, PMNs contribute to resilience and tissue homeostasis, with continuous PMN-mediated actions required for the maintenance of health, particularly in mucosal tissues. PMNs are extraordinarily well-adapted to respond to and diminish the damaging effects of a vast repertoire of infectious agents and injurious processes that are encountered throughout life. The commensal biofilm, a symbiotic polymicrobial ecosystem that lines the mucosal surfaces, is the first line of defense against pathogenic strains that might otherwise dominate, and is therefore of critical importance for health. PMNs regularly interact with the commensal flora at the mucosal tissues in health and limit their growth without developing an overt inflammatory reaction to them. These PMNs exhibit what is called a para-inflammatory phenotype, and have reduced inflammatory output. When biofilm growth and makeup are disrupted (i.e., dysbiosis), clinical symptoms associated with acute and chronic inflammatory responses to these changes may include pain, erythema and swelling. However, in most cases, these responses indicate that the immune system is functioning properly to re-establish homeostasis and protect the status quo. Defects in this healthy everyday function occur as a result of PMN subversion by pathological microbial strains, genetic defects or crosstalk with other chronic inflammatory conditions, including cancer and rheumatic disease, and this can provide some avenues for therapeutic targeting of PMN function. In other cases, targeting PMN functions could worsen the disease state. Certain PMN-mediated responses to pathogens, for example Neutrophil Extracellular Traps (NETs), might lead to undesirable symptoms such as pain or swelling and tissue damage/fibrosis. Despite collateral damage, these PMN responses limit pathogen dissemination and more severe damage that would otherwise occur. New data suggests the existence of unique PMN subsets, commonly associated with functional diversification in response to particular inflammatory challenges. PMN-directed therapeutic approaches depend on a greater understanding of this diversity. Here we outline the current understanding of PMNs in health and disease, with an emphasis on the positive manifestations of tissue and organ-protective PMN-mediated inflammation.

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A Novel Standardized Cannabis sativa L. Extract and Its Constituent Cannabidiol Inhibit Human Polymorphonuclear Leukocyte Functions

Cited by 24 publications

References 48 publications

Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients

Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients

Cannabidiol for Viral Diseases: Hype or Hope?

The Neutrophil: Constant Defender and First Responder

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