A novel steroid and cytotoxic constituents from Dioscorea membranacea Pierre against hepatocellular carcinoma and cholangiocarcinoma cells

Thongdeeying, Pakakrong; Itharat, Arunporn; Umehara, Kaoru; Ruangnoo, Srisopa

doi:10.1016/j.jep.2016.08.037

Cited by 11 publications

(18 citation statements)

References 12 publications

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“…The putative active compound of Benja-ummarit is gambogic acid, whereas dioscorealide B is candidate for active principle in D. membranacea extracts. Dioscorealide B reportedly has a high cytotoxic effect for human liver cancer cells 25 and a pro-apoptotic activity in breast cancer cells. 28 Moreover, our research group found that HCC-bearing rats treated with a crude extract of D. membranacea showed DNA fragmentation by TUNEL assay, indicating apoptosis of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…22 , 23 Briefly, 13 compounds are known to be present in ethanolic DM extract including I) dioscorealides A, II) dioscorealides B, III) dioscoreanone, 19 , 24 IV) β-sitosterol, V) stigmasterol, VI) β-D-sitosterol glucoside, VII) diosgenin-(3-O-α- l -rhamnopyranosyl (1 → 2)-β- d -glucopyranoside, VIII) diosgenin 3-O-β- d -glucopyranosyl (1 → 3)-β- d -glucopyranoside, 19 , 20 IX) 2,4 dimethoxy-5,6-dihydroxy-9,10-dihydrophenanthrene, X) 5-hydroxy-2,4,6-trimethoxy-9,10-dihydrophenanthrene, and XI) 5,6,2 -trihydroxy 3,4-methoxy, 9,10-dihydrophenanthrene, 14 XII) epipanthogenin B, and XIII) panthogenin B. 25 The working solution of DM was prepared in propylene glycol:tween80:water (4:1:4 v/v).…”

Section: Methodsmentioning

confidence: 99%

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Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Kerdput

Nilbu-Nga

Kaewnoonual

et al. 2021

Journal of Traditional and Complementary Medicine

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Hepatocellular carcinoma (HCC) is most common in adults and has a high mortality rate because of a lack of effective treatment options. We investigated the effect of a medicinal plant as a potential source of drugs against HCC. The rhizomes of Dioscorea membranacea Pierre (DM), Hua-Khao-Yen in Thai, are commonly used as ingredients for alternative treatment of cancer in Thailand. In this study, the anticancer effects of DM extract in HCC-bearing rats were evaluated with respect to gross morphology, histopathology, and leakage of liver enzymes. In untreated HCCs, typical features of liver cancer, including hepatic nodules, thick-cell cords, and pseudoglandular cell arrangements, were observed. In addition, the HCCs showed abnormal reticulin patterns and a high glypican3 expression. In HCC-bearing rats treated with DM the cancer areas and reticulin expression were significantly reduced compared to the untreated group ( p < 0.01). Sorafenib, the standard drug to treat HCC, reduced the cancer area further, but increased leakage of liver enzymes and decreased serum albumin concentration, indicating liver toxicity. These findings suggest that DM has an anticancer effect on HCCs in an animal model in vivo with potentially less severe side effects than sorafenib. Therefore, further studies of DM’s mechanism of action in HCC should be carried out.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Kerdput

Nilbu-Nga

Kaewnoonual

et al. 2021

Journal of Traditional and Complementary Medicine

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“…The use of active compounds naphthofuranoxepins (dioscorealide A and B) and dihydrophenanthrene from D. membranacea (locally known as Hua-Khao-yen) rhizome in Thai medicine is highly potent and has exhibited cytotoxic activity against five types of human cancer cells [274][275][276]. This was supported by a more recent study, which highlighted the utilization of dioscorealide B as a possible anticancer agent for liver cancer and cholangiocarcinoma [277]. The hepatotoxic compound diosbulbin B has also been reported as a major antitumor bioactive component of D. bulbifera (air potato) in dose-dependent manner, with no significant toxicity in vivo at dosage between 2 and 16 mg/kg [278,279].…”

Section: Anticancer Activity Of Yammentioning

confidence: 96%

The Dioscorea Genus (Yam)—An Appraisal of Nutritional and Therapeutic Potentials

Obidiegwu

Lyons

Chilaka

2020

Foods

142

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The quest for a food secure and safe world has led to continuous effort toward improvements of global food and health systems. While the developed countries seem to have these systems stabilized, some parts of the world still face enormous challenges. Yam (Dioscorea species) is an orphan crop, widely distributed globally; and has contributed enormously to food security especially in sub-Saharan Africa because of its role in providing nutritional benefits and income. Additionally, yam has non-nutritional components called bioactive compounds, which offer numerous health benefits ranging from prevention to treatment of degenerative diseases. Pharmaceutical application of diosgenin and dioscorin, among other compounds isolated from yam, has shown more prospects recently. Despite the benefits embedded in yam, reports on the nutritional and therapeutic potentials of yam have been fragmented and the diversity within the genus has led to much confusion. An overview of the nutritional and health importance of yam will harness the crop to meet its potential towards combating hunger and malnutrition, while improving global health. This review makes a conscious attempt to provide an overview regarding the nutritional, bioactive compositions and therapeutic potentials of yam diversity. Insights on how to increase its utilization for a greater impact are elucidated.

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“…The crude extracts and isolated compounds were evaluated for cytotoxic activity by using sulforhodamine B (SRB) assay against two cancerous liver cells (HepG2 and KKU-M156) and a normal cells (HaCaT). The SRB protocol has been described previously [14]. HepG2 was cultured in MEM; KKU-M156 was cultured in HAM F-12 media and HaCaT was cultured in DMEM.…”

Section: Methodsmentioning

confidence: 99%

Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

Pipatrattanaseree

Itharat

Mukkasombut

et al. 2019

BMC Complement Altern Med

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BackgroundThe root of Baliospermum montanum has been used as an ingredient of traditional Thai medicines for the treatments of several diseases including itching eczema, muscle and joint inflammation, and cancer. Few studies have been done on phytochemical components of this root. In this study, we isolated major compounds of the crude ethanolic extract of B. montanum root and developed and validated a high performance liquid chromatographic (HPLC) method for the determination of its major components. We then investigated anti-allergic, anti-inflammatory and cytotoxic activities of the extract.MethodsThe aims of this study were to investigate in vitro activities including inhibitory effect of β-hexosaminidase released from RBL-2H3 cells, inhibition of nitric oxide (NO) production from RAW 264.7 cells and cytotoxic activity against cancerous liver cell lines (HepG2 and KKU M156) by using sulforhodamine B (SRB) assay. Isolation of major components was conducted by using column chromatographic method. Isolated major compounds were analyzed by using high performance liquid chromatography (HPLC).ResultsThe crude extract exhibited the highest cytotoxic activity, with IC50 less than 1 μg/mL, while its anti-allergy and anti-inflammation were also potent with IC50 less than 6 μg/mL. Three propiophenones isolated from B. montanum root exhibited moderate cytotoxic activities (IC50 > 20 μg/mL). Two of the propiophenones found were major components that can be detected by HPLC. The developed and validated HPLC method showed good accuracy, precision, and linearity.ConclusionThe results of this study suggested that ethanolic extract of of B.montanum root can be a potential source of anti-allergy, anti-inflammation, and anti-cancer compounds. The isolated compounds can serve as markers when B. montanum is used in herbal remedies but not as overall responsive markers. The HPLC method developed may be useful for quality control in the production of the extract and for further formulation developments. However, investigation of several associated biological activities is necessary before the development can proceed further. Minor active compounds should be isolated and a more sensitive analytical method should be developed to detail the key responsive components of the ethanolic extract of B. montanum root.Electronic supplementary materialThe online version of this article (10.1186/s12906-019-2449-0) contains supplementary material, which is available to authorized users.

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A novel steroid and cytotoxic constituents from Dioscorea membranacea Pierre against hepatocellular carcinoma and cholangiocarcinoma cells

Cited by 11 publications

References 12 publications

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

Therapeutic efficacy of a Dioscorea membranacea extract in a rat model of hepatocellular carcinoma: Histopathological aspects

The Dioscorea Genus (Yam)—An Appraisal of Nutritional and Therapeutic Potentials

Potential in vitro anti-allergic, anti-inflammatory and cytotoxic activities of ethanolic extract of Baliospermum montanum root, its major components and a validated HPLC method

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