A Novel Strain of Probiotic Leuconostoc citreum Inhibits Infection-Causing Bacterial Pathogens

Muthusamy, Karnan; Han, Hyo-Shim; Ilavenil, Soundharrajan; Jung, Ji Yun; Arasu, Mariadhas Valan; Choi, Ki-Seok

doi:10.3390/microorganisms11020469

Cited by 7 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular analyses afford heightened precision in the identification of microorganisms possessing optimal probiotic characteristics. The identification of L. citreum , characterized as Gram-positive cocci with negative catalase activity, aligns with reports highlighting its probiotic capacity to inhibit pathogenic microorganisms ( Muthusamy et al , 2023 ; Ahn et al , 2023 ). Members of this genera also have been isolated from fermented Torshi showing a high acid and bile tolerance ( Nemati et al , 2023 ).…”

Section: Discussionsupporting

confidence: 82%

Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)

Vargas,

Alor,

Pachorro

et al. 2024

Open Vet J

View full text Add to dashboard Cite

Background: Presently, there exists a growing interest in mitigating the utilization of antibiotics in response to the challenges emanating from their usage in livestock. A viable alternative strategy encompasses the introduction of live microorganisms recognized as probiotics, exerting advantageous impacts on the immune system and nutritional aspects of the host animals. Native lactic acid bacteria, inherently possessing specific properties and adaptive capabilities tailored to each animal, are deemed optimal contenders for probiotic advancement. Aim: In the current investigation, microorganisms exhibiting probiotic potential were isolated, characterized, and identified from the fecal samples of guinea pigs (Cavia porcellus) belonging to the Peruvian breed. Methods: The lactic acid bacteria isolated on MRS agar underwent Gram staining, catalase testing, proteolytic, amylolytic, and cellulolytic activity assays, low pH tolerance assessment, hemolytic evaluation, antagonism against Salmonella sp., determination of autoaggregation and coaggregation capacity, and genotypic characterization through sequencing of the 16S rRNA gene. Results: A total of 33 lactic acid bacteria were isolated from the feces of 30 guinea pigs, with 10 selected based on Gram staining and catalase testing. All strains exhibited proteolytic activity, although only one demonstrated amylolytic capability, while none displayed cellulase activity. These bacteria demonstrated higher tolerance to pH 5.0 and, to a lesser extent, to pH 4.0. Furthermore, they exhibited antagonistic activity against Salmonella sp. Only 2 bacteria demonstrated hemolytic activity, and they were subsequently excluded from further evaluations. Subsequent assessments revealed autoaggregation capacities ranging from 4.55% to 23.19%, with a lesser degree of coaggregation with Salmonella sp. ranging from 3.53% to 8.94% for the remaining 8 bacterial isolates. Based on these comprehensive tests, 5 bacteria with notable probiotic potential were identified through molecular assays, belonging to the groups Leuconostoc citreum, Enterococcus gallinarum, Exiguobacterium sp., and Lactococcus lactis. Conclusion: The identified bacteria stand out as promising probiotic candidates, deserving further assessment in Peruvian breed guinea pigs. This exploration aims to enhance production outcomes while mitigating the adverse effects induced by pathogenic microorganisms.

show abstract

Section: Discussionsupporting

confidence: 82%