A novel strategy for acetonitrile wastewater treatment by using a recombinant bacterium with biofilm-forming and nitrile-degrading capability

Li, Chunyan; Yue, Zhenlei; Feng, Fengzhao; Xi, Chuanwu; Zang, Hailian; An, Xiaopeng; Liu, Keran

doi:10.1016/j.chemosphere.2016.07.019

Cited by 28 publications

(5 citation statements)

References 34 publications

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“…For example, acetonitrile, a water-soluble substrate that can be converted to toxic hydrogen cyanide in human and animal cells, is a common contaminant in wastewater of the chemical industry (Greenberg, 1999;Robles, 2014). It has been shown that the biofilm-forming strain B. subtilis N4-pHT01-nit strain expressing the Rhodococcus rhodochrous nitrilase Nit was not only resistant to acetonitrile, but it was able to degrade the molecule (most of the added 800 mg l À1 within 24 h; Li et al, 2016b). This approach shows how simple a biotechnological application can be.…”

Section: Discussionmentioning

confidence: 99%

The contribution of bacterial genome engineering to sustainable development

Reuß

Commichau

Stülke

2017

Microbial Biotechnology

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SummaryThe United Nations’ Sustainable Development Goals define important challenges for the prosperous development of mankind. To reach several of these goals, among them the production of value‐added compounds, improved economic and ecologic balance of production processes, prevention of climate change and protection of ecosystems, the use of engineered bacteria can make valuable contributions. We discuss the strategies for genome engineering and how they can be applied to meet the United Nations’ goals for sustainable development.

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Section: Discussionmentioning

confidence: 99%

The contribution of bacterial genome engineering to sustainable development

Reuß

Commichau

Stülke

2017

Microbial Biotechnology

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“…(A) B. subtilis adhesion and biofilm formation on modified basalt fibers (MBF) bio-carrier for wastewater treatment [ 72 ]. (B) Acetonitrile degrading capability of B. subtilis N4-pHT01-nit after waste water treatment [ 79 ]. (C) Cell growth of B. subtilis N4/pHTnha-ami and organonitriles (acetonitrile, acrylonitrile, crotononitrile) degradation by NHase, amidase and nitrilase genes [ 80 ].…”

Section: Engineered B Subtilis Biofilms For Bioremediationmentioning

confidence: 99%

“…This genetically engineered strain was constructed by cloning a novel nitrilase ( nit ) gene from bacterium, Rhodococcus rhodochrous BX2 that degrade toxic compound nitrile into a biofilm-forming bacterium B. subtilis N4, displayed recombinant protein upon IPTG induction ( Fig. 4 B) [ 79 ]. Further, Li et al [ 80 ] cloned Nitrile hydratase ( nha ) and amidase ( ami ) genes into the B. subtilis N4 which shows a strong biofilm forming ability and construct a genetically modified B. subtilis N4/pHTnha-ami, which completely degrade organonitriles from the wastewater.…”

Section: Engineered B Subtilis Biofilms For Bioremediationmentioning

confidence: 99%

Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

Mohsin

Omer

Huang

et al. 2021

Synthetic and Systems Biotechnology

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Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and biotechnology. B. subtilis is capable of producing both biofilms and spores. Biofilms are matrix-encased multicellular communities that comprise various components including exopolysaccharides, proteins, extracellular DNA, and poly-γ-glutamic acid. These biofilms resist environmental conditions such as oxidative stress and hence have applications in bioremediation technologies. Furthermore, biofilms and spores can be engineered through biotechnological techniques for environmentally-friendly and safe production of bio-products such as enzymes. The ability to withstand with harsh conditions and producing spores makes Bacillus a suitable candidate for surface display technology. In recent years, the spores of such specie are widely used as it is generally regarded as safe to use. Advances in synthetic biology have enabled the reprogramming of biofilms to improve their functions and enhance the production of value-added products. Globally, there is increased interest in the production of engineered biosensors, biocatalysts, and biomaterials. The elastic modulus and gel properties of B. subtilis biofilms have been utilized to develop living materials. This review outlines the formation of B. subtilis biofilms and spores. Biotechnological engineering processes and their increasing application in bioremediation and biocatalysis, as well as the future directions of B. subtilis biofilm engineering, are discussed. Furthermore, the ability of B. subtilis biofilms and spores to fabricate functional living materials with self-regenerating, self-regulating and environmentally responsive characteristics has been summarized. This review aims to resume advances in biological engineering of B. subtilis biofilms and spores and their applications.

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“…For instance, the contaminant-degrading bacteria may not be efficiently immobilised in biofilms and may therefore be easily washed out of the system, while the bacteria that create biofilms may not successfully remove pollutants. Additionally, simply mixing biofilm forming bacteria with degrading bacteria may result in mutual growth inhibition, slow biofilm development, difficult operation, and other problems (Li et al, 2016). Both the fixation of functional bacteria in the wastewater treatment system and high-efficiency pollutant removal can be accomplished by the presence of a single bacterial species with the capacity to effectively form biofilms and breakdown pollutants simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Biopolishing of Domestic Wastewater Using Polyvinyl Alcohol – Supported Biofilm of Bacterial Strain <i>Bacillus velezensis</i> Isolate JB7

Othman¹,

Hasan²,

Muhamad³

et al. 2023

J. Ecol. Eng.

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Water pollution occurs due to the discharge of domestic waste mixed with residential, industrial, commercial, and agricultural wastewater. Conventional water treatment methods using aerobic/anaerobic methods can cause problems with the production of high green gases and result in the greenhouse effect. Microbial-based domestic sewage treatment technology using polyvinyl alcohol biofilm supporting media was introduced as an alternative measure to overcome this problem. The objective of the study was to determine the performance of polyvinyl alcohol beads in polishing domestic wastewater. In this study, the bacterium Bacillus velezensis isolate JB7 was used together with PVA as a raw material to treat domestic sewage wastewater more efficiently and stably. The results of the study show the effectiveness of domestic wastewater treatment in several factors such as pH value, chemical oxygen demand, phosphorus, nitrate, nitrite, ammonia, and total suspended solids. As conclusion, domestic wastewater treatment methods using polyvinyl alcohol beads are seen to be effective, reducing the use of sewage waste plant construction sites and able to avoid the use of non-recyclable materials such as plastics and synthetics.

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A novel strategy for acetonitrile wastewater treatment by using a recombinant bacterium with biofilm-forming and nitrile-degrading capability

Cited by 28 publications

References 34 publications

The contribution of bacterial genome engineering to sustainable development

The contribution of bacterial genome engineering to sustainable development

Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

Biopolishing of Domestic Wastewater Using Polyvinyl Alcohol – Supported Biofilm of Bacterial Strain <i>Bacillus velezensis</i> Isolate JB7

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