A novel strategy for cartilage tissue engineering: Collagenase-loaded cryogel scaffolds in a sheep model

Gürer, Burak; Yılmaz, Cengiz; Yılmaz, Şükran; Çabuk, Sertan; Bölgen, Nimet

doi:10.1080/00914037.2017.1327433

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…Following washing, the slides were labeled with secondary antibody (anti-mouse IgG, ab2891; Abcam) and co-stained with the pan-nuclear marker DAPI (1:1000 dilution). Representative images of each section were taken using an Olympus uorescent microscope (Olympus BX51) equipped with a digital camera [50].…”

Section: Immunocytochemistrymentioning

confidence: 99%

Chondrogenesis of mesenchymal stromal cells on the 3D printed polycaprolactone/fibrin/decellular cartilage matrix hybrid scaffolds in the presence of piascledine

Honarvar,

Setayeshmehr,

Ghaedamini

et al. 2024

Journal of Biomaterials Science, Polymer Edition

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Today, cartilage tissue engineering (CTE) is considered important due to the lack of repair of cartilaginous lesions and the absence of appropriate methods for treatment. In this study, polycaprolactone (PCL) scaffolds were fabricated by three-dimensional (3D) printing and were then coated with brin (F) and acellular solubilized extracellular matrix (ECM). After extracting adipose-derived stem cells (ADSCs), 3Dprinted scaffolds were characterized and compared to hydrogel groups. After inducing the chondrogenic differentiation in the presence of Piascledine and comparing it with TGF-β3 for 28 days, the expression of genes involved in chondrogenesis (AGG, COLII) and the expression of the hypertrophic gene (COLX) were examined by real-time PCR. The expression of proteins COLII and COLX was also determined by immunohistochemistry. Glycosaminoglycan was measured by toluidine blue staining. 3D-printed scaffolds clearly improved cell proliferation, viability, water absorption, and compressive strength compared to the hydrogel groups. Moreover, the use of compounds such as ECM and Piascledine in the process of ADSCs chondrogenesis induction increased cartilage-speci c markers and decreased the hypertrophic marker compared to TGF-β3. In Piascledine groups, the expression of COLL II protein, COLL II and Aggrecan genes, and the amount of glycosaminoglycan showed a statistically signi cant increase in the PCL/F/ECM compared to the PCL and PCL/F groups (P < 0.05). The results con rmed that the PCL/F/ECM scaffolds presented in this study afford unique opportunities for CTE.

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Section: Immunocytochemistrymentioning

confidence: 99%

Chondrogenesis of mesenchymal stromal cells on the 3D printed polycaprolactone/fibrin/decellular cartilage matrix hybrid scaffolds in the presence of piascledine

Honarvar,

Setayeshmehr,

Ghaedamini

et al. 2024

Journal of Biomaterials Science, Polymer Edition

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“…To overcome these limits, Gel is frequently chemically cross-linked and combined with other polymers. In particular, to improve the thermal stability, Gel has been chemically cross-linked with GA [73][74][75][76]. However, due to the toxicity of this cross-linking agent, alternative dialdehydes have been proposed to produce stable and mechanical-resistant Gel-based networks, such as pullulan dialdehyde [26] and dextran dialdehyde [6], obtained from the oxidation of pullulan and dextran, respectively.…”

Section: Gelatin-based Cryogelsmentioning

confidence: 99%

“…For this reason, they can be barely used alone for the fabrication of cryogels intended for tissueengineering purposes, whereas they are frequently proposed in combination with other biopolymers [55]. GA [74] GA [75] HA GA [76] bioglass APS/TEMED radical cross-linking [38] bone morphogenic protein-2 APS/TEMED radical cross-linking [77] poly(lactic-co-glycolic acid) HA EDC/NHS [66] HA EDC/NHS [103] heparin EDC/NHS [48] UV photocuring [39] BMP-2 biomimetic peptide and VEGF APS/TEMED [37] hyaluronic acid; chondroitin sulfate EDC/NHS [88] carboxymethyl chitosan EDC/NHS [49] APS/TEMED radical cross-linking [9] alginate Ca(II) DVS [55] Silk fibroin strength; toughness; mechanical stress resistance; mechanical flexibility; gluconic acid EDC/NHS [17] Silk fibroin Ag/Sr doped HA physical cross-linking [18] agarose HA physical cross-linking [108] gelatin Ce/Zn doped HA GA [73] gelatin HA GA [44] GA [58] gelatin EDC/NHS [49] Nano cellulose micro-structuring silica [98] 4.6. Synthetic Polymers Several synthetic polymers have been investigated for the development of scaffolds for bone or cartilage regeneration, including polyethylene glycol (PEG), polyhydroxyethylor polyhydroxypropyl-methacrylate (p-HEMA; p-HPMA), and polyvinyl alcohol (PVA).…”

Section: Other Natural Polymersmentioning

confidence: 99%

Cryogel Scaffolds for Tissue-Engineering: Advances and Challenges for Effective Bone and Cartilage Regeneration

Carriero,

Di Muzio,

Petralito

et al. 2023

Gels

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Critical-sized bone defects and articular cartilage injuries resulting from trauma, osteonecrosis, or age-related degeneration can be often non-healed by physiological repairing mechanisms, thus representing a relevant clinical issue due to a high epidemiological incidence rate. Novel tissue-engineering approaches have been proposed as an alternative to common clinical practices. This cutting-edge technology is based on the combination of three fundamental components, generally referred to as the tissue-engineering triad: autologous or allogenic cells, growth-stimulating factors, and a scaffold. Three-dimensional polymer networks are frequently used as scaffolds to allow cell proliferation and tissue regeneration. In particular, cryogels give promising results for this purpose, thanks to their peculiar properties. Cryogels are indeed characterized by an interconnected porous structure and a typical sponge-like behavior, which facilitate cellular infiltration and ingrowth. Their composition and the fabrication procedure can be appropriately tuned to obtain scaffolds that match the requirements of a specific tissue or organ to be regenerated. These features make cryogels interesting and promising scaffolds for the regeneration of different tissues, including those characterized by very complex mechanical and physical properties, such as bones and joints. In this review, state-of-the-art fabrication and employment of cryogels for supporting effective osteogenic or chondrogenic differentiation to allow for the regeneration of functional tissues is reported. Current progress and challenges for the implementation of this technology in clinical practice are also highlighted.

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“…Cartilage damages could be treated by autologous chondrocyte implantation (ACI) in which two operations even one is open are necessary with additional disadvantages of high cost, morbidity, iatrogenic pneumothorax atelectasis. Therefore, it is a requirement to use scaffolds including cryogels as a better alternative for the treatment [71]. In this field, cryogels could be modified by additives to enhance their applicability and they have also been implanted to observe their ability in treatment of cartilage damages.…”

Section: Scaffoldsmentioning

confidence: 99%

Biomedical Applications of Polymeric Cryogels

et al. 2019

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The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over traditional gel carriers in the field of biochromatography and related biomedical applications. These matrices nearly mimic the three-dimensional structure of native tissue extracellular matrix. In addition, mechanical, osmotic and chemical stability of cryogels make them attractive polymeric materials for the construction of scaffolds in tissue engineering applications and in vitro cell culture, separation materials for many different processes such as immobilization of biomolecules, capturing of target molecules, and controlled drug delivery. The low mass transfer resistance of cryogel matrices makes them useful in chromatographic applications with the immobilization of different affinity ligands to these materials. Cryogels have been introduced as gel matrices prepared using partially frozen monomer or polymer solutions at temperature below zero. These materials can be produced with different shapes and are of interest in the therapeutic area. This review highlights the recent advances in cryogelation technologies by emphasizing their biomedical applications to supply an overview of their rising stars day to day.

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A novel strategy for cartilage tissue engineering: Collagenase-loaded cryogel scaffolds in a sheep model

Cited by 10 publications

References 35 publications

Chondrogenesis of mesenchymal stromal cells on the 3D printed polycaprolactone/fibrin/decellular cartilage matrix hybrid scaffolds in the presence of piascledine

Chondrogenesis of mesenchymal stromal cells on the 3D printed polycaprolactone/fibrin/decellular cartilage matrix hybrid scaffolds in the presence of piascledine

Cryogel Scaffolds for Tissue-Engineering: Advances and Challenges for Effective Bone and Cartilage Regeneration

Biomedical Applications of Polymeric Cryogels

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