A novel strigolactone receptor antagonist provides insights into the structural inhibition, conditioning, and germination of the crop parasite Striga

Arellano-Saab, Amir; McErlean, Christopher S. P.; Lumba, Shelley; Savchenko, Alexei; Stogios, P.J.; McCourt, Peter

doi:10.1016/j.jbc.2022.101734

Cited by 9 publications

(6 citation statements)

References 62 publications

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“…The (±)-YLG has also been used to validate SL receptor agonists (Uraguchi et al, 2018 ) or antagonists (Holbrook-Smith et al, 2016 ; Arellano-Saab et al, 2022 ; Zarban et al, 2022 ) for Striga . Again, the discovery of a profluorescent probe, which is as active as SLs, remains to be discovered to obtain a more relevant screening tool for the discovery of efficient inhibitors for SL receptors or SL mimics.…”

Section: Discussionmentioning

confidence: 99%

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

et al. 2022

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Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.

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Section: Discussionmentioning

confidence: 99%

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

et al. 2022

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“…The SL signaling pathway is putatively the same for root parasitic plant seed germination, although not all protein targets have yet been identified [130] . In addition, for SL receptors in Striga, internal tunnels were detected after ligand perception explaining how SL exits after cleavage [131] . The enzymatic activity of receptors towards SLs enables these organisms to be hypersensitive to SLs for germination.…”

Section: Perception Of Sls and Sl Receptor Inhibitorsmentioning

confidence: 99%

Chemistry of Strigolactones, Key Players in Plant Communication

Daignan‐Fornier,

Keita,

Boyer

2024

ChemBioChem

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Today, the use of artificial pesticides is questionable and the adaptation to global warming is a necessity. The promotion of favorable natural interactions in the rhizosphere offers interesting perspectives for changing the type of agriculture. Strigolactones (SLs), the latest class of phytohormones to be discovered, are also chemical mediators in the rhizosphere. We present in this review the diversity of natural SLs, their analogs, mimics, and probes essential for the biological studies of this class of compounds. Their biosynthesis and access by organic synthesis are highlighted especially concerning noncanonical SLs, the more recently discovered natural SLs. Organic synthesis of analogs, stable isotope‐labeled standards, mimics, and probes are also reviewed here. In the last part, the knowledge about the SL perception is described as well as the different inhibitors of SL receptors that have been developed.

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“…Through structural analysis, the authors recognized the binding mechanism of DOZ 199 being novel and distinct from that of known SLs. 173 Using SL-hypocotyl-shortening assays, the authors had previously identified several compounds that appeared to act as SL antagonists interacting with SL receptor ShHTL7. 174 In particular, piperazine-based analogues 207−211 have been identified as target compounds showing optimized binding into the active site of ShHTL7.…”

Section: ■ Strigolactonesmentioning

confidence: 99%

“…Remarkably, its chemical structure shows very little similarity to that of known SLs; namely, it has a piperazine core structure and the D-ring is absent (Figure ). Through structural analysis, the authors recognized the binding mechanism of DOZ 199 being novel and distinct from that of known SLs . Using SL-hypocotyl-shortening assays, the authors had previously identified several compounds that appeared to act as SL antagonists interacting with SL receptor ShHTL7 …”

Section: Strigolactonesmentioning

confidence: 99%

Inspired by Nature: Isostere Concepts in Plant Hormone Chemistry

Frackenpohl

Abel

Alnafta

et al. 2023

J. Agric. Food Chem.

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Chemical concepts such as isosteres and scaffold hopping have proven to be powerful tools in agrochemical innovation processes. They offer opportunities to modify known molecular lead structures with the aim to improve a range of parameters, including biological efficacy and spectrum, physicochemical properties, stability, and toxicity. While recent biochemical insights into plant-specific receptors and signaling pathways trigger the discovery of the first lead structures, the disclosure of such a new chemical structure sparks a broad range of synthesis activities giving rise to diverse chemical innovation and often a considerable boost in biological activity. Herein, recent examples of isostere concepts in plant-hormone chemistry will be discussed, outlining how synthetic creativity can broaden the scope of natural product chemistry and giving rise to new opportunities in research fields such as abiotic stress tolerance and growth promotion.

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A novel strigolactone receptor antagonist provides insights into the structural inhibition, conditioning, and germination of the crop parasite Striga

Cited by 9 publications

References 62 publications

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Chemistry of Strigolactones, Key Players in Plant Communication

Inspired by Nature: Isostere Concepts in Plant Hormone Chemistry

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