A novel support for enzyme adsorption: properties and applications of aerogels in low water media

Basso, Alessandra; Martin, Luigi De; Ebert, Cynthia; Gardossi, Lucia; Tomat, Alida; Casarci, M.; Rosi, Ornella Li

doi:10.1016/s0040-4039(00)01522-7

Cited by 23 publications

(12 citation statements)

References 8 publications

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“…[67] When applied to penicillin G acylase, the activity recovery was a poor 10%, which the authors ascribed to diffusion limitation in the silica matrix. [68] We note that penicillin G acylase is inherently more sensitive to diffusion limitation than, for example, a lipase. Moreover, the procedure has not been designed for fast mass transport, because this issue is of little relevance considering the generally quite low turnover rate of enzymes in organic solvents.…”

Section: Entrapment In a Silica Matrixmentioning

confidence: 94%

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

2005

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Penicillin G acylase is a major industrial biocatalyst that is used in the enzymatic production of 20,000 t a À 1 of 6-aminopenicillanic acid, the industrial b-lactam intermediate, as well as in the enzymatic production of semi-synthetic b-lactam antibiotics. Because efficient recovery and reuse of the biocatalyst is a prerequisite for a viable process, much attention has been focused on the immobilization of penicillin G acylase. Methods that have been studied and will be discussed in this review include covalent attachment to porous organic and inorganic carriers, inclusion in and attachment to biopolymer gels and carrier-free immobilization techniques. Highly active and stable preparations have been developed; mass transfer limitations in the carrier are now a major barrier to further improvement of the biocatalyst performance.

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Section: Entrapment In a Silica Matrixmentioning

confidence: 94%

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

2005

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“…The condensation leads to the formation of a gel phase. To convert a gel into an aerogel, the solvent is removed by extraction with supercritical CO 2 or by the direct conversion of the solvent in supercritical state in order to prevent the structure collapse caused by capillary forces [Fricke 1986]. The chemistry of aerogel materials is relatively f lexible: their pore size and surface area can be tailored during the synthesis by changing the solvent and catalysts [Rao et al 1993, Pajonk 1998, Stolarski et al 1999].…”

Section: Introductionmentioning

confidence: 99%

Aerogels: Tailor-made Carriers for Immediate and Prolonged Drug Release

Смирнова

Suttiruengwong

Arlt

2005

KONA

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“…The encapsulation of enzymes during a sol-gel process also seems to be more beneficial compared to the simple adsorption of enzymes on the aerogels after supercritical drying (post-treatment). Basso et al [24] report that the catalytical activity of the enzymes PGA, thermolysin, and chymotrypsin -adsorbed on aerogels by soaking in the enzyme suspensionis rather low. It might be due to the fact that the enzymes are not really integrated into the aerogel structure, so that aggregation cannot be avoided.…”

Section: Silica Aerogels As Carriers For Enzymes and Proteinsmentioning

confidence: 99%

Pharmaceutical Applications of Aerogels

Смирнова

2011

Aerogels Handbook

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At present, an interest has grown in the field of biocompatible aerogels and composite aerogel materials in life science, especially in biomedical and pharmaceutical applications. Due to their large surface area, open pore structure, and biocompatibility, aerogels are very promising candidates for drug delivery systems. The use of both inorganic and organic aerogels as carriers for pharmaceutically active compounds is discussed in this chapter. Both the stability and the release kinetics of the drug can be significantly improved by loading them into aerogels. First attempts to prepare semisolid and solid pharmaceutical formulations have been made. Furthermore, aerogels can be used as a host matrix for bioactive compounds (enzymes and proteins), which improve or enable their performance. Taking into account all research activities in the area of aerogels, a number of promising pharmaceutical applications can be expected in future. IntroductionSilica aerogels were used in daily life products since 1960s, when Monsanto's aerogels were introduced as additives for cosmetics and toothpaste. At present, an interest has grown in the field of biocompatible aerogels and composite aerogel materials in life sciences, especially for biomedical and pharmaceutical applications. In principle, biocompatible aerogels can be made from any organic compound, whose properties such as toxicity and biodegradation are suitable for the given application. In the case of aerogel composites, the final material consists of an aerogel matrix and one or more additional phases (of any composition or scale), which influence the properties of the final product. Thus, at least one phase has a physical structure with dimensions in the order of nanometres (the particles and pores of the aerogel). The aerogel composites can be prepared in two different ways: through the addition of a target compound during the sol-gel process (Figure 31.1A) and by post-treatment of the dried aerogels (Figure 31.1B), for example, by adsorption, vapour phase deposition, or reactive gas treatment.The first method (a) is attractive by its simplicity and flexibility, since virtually every material can be easily added to the sol solution before gelation. This added material can be of I. Smirnova

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A novel support for enzyme adsorption: properties and applications of aerogels in low water media

Cited by 23 publications

References 8 publications

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

Aerogels: Tailor-made Carriers for Immediate and Prolonged Drug Release

Pharmaceutical Applications of Aerogels

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