A novel surface-enhanced Raman scattering (SERS) strategy for ultrasensitive detection of bacteria based on three-dimensional (3D) DNA walker

Overview of Rapid Detection Methods for Salmonella in Foods: Progress and Challenges

“…The Raman spectrum is a scattering spectrum which provides unique fingerprint information on different substances according to molecular vibration, and the Raman shift can describe the spectral information of different molecules [68]. SERS is an optical sensing technology that uses roughened metal surfaces or special nanomaterials to enhance Raman scattering and improve the detection sensitivity.…”

An enzyme-mediated universal fluorescent biosensor template for pathogen detection based on a three-dimensional DNA walker and catalyzed hairpin assembly

“…44 In addition, there are some methods that reach a lower LOD than this work (Table S2b †). For example, the reported PCR, 7 immunology-based assays 45 and surfaceenhanced Raman scattering (SERS) 46 methods have realized the sensitive detection of S. ty, and their LODs have reached 7-9, 10 and 4 CFU mL −1 , respectively. However, the PCR method requires expensive equipment and has a longer detection time; immunology-based assays are faced with the challenge of cross-reaction with closely related antigens and antigen variation.…”

“…Although SERS can achieve low-cost and ultra-sensitive bacterial detection, the reporter molecule used in the SERS method shows obvious signal attenuation during the detection process. 46 Through the comparison of Michaelis-Menten equation constants and detection limit, it was found that a system with an optimal PolyA tail has an appropriate density and conformation of the DNA probe, thus improving the collision probability and efficiency of Nt.BbvCI, leading to a low detection limit. And based on the above experimental phenomena, PolyA 10 -DNA achieves the optimal performance for the detection of S. ty in this fluorescent biosensor.…”

Applications of surface‐enhanced Raman spectroscopy in environmental detection

“…NPL drop cast onto Au-functionalized glass slides 10 μg/mL [115] Silver nanoparticles on regenerated cellulose Crystal violet and polystyrene Analyte deposited on SERS substrate 0.1 mg/mL [62] Silver @ gold nanostars @ anodized aluminum oxide Polystyrene Tap, river and sea water 0.05 mg/g [116] Gold nanoparticles Polystyrene PS spiked in water - [114] Gold nanoparticles @ 4-MBN Melamine Milk 10 nM [265] Silver nanoparticles S. aureus and E. coli Substrate - [235] Gold modified magnetic nanoparticles Salmonella Spiked: chicken and milk Natural: Milk, tap water, poor water 4 cfu/mL [229] Silver/copper oxide nanowires/pyramidal PDMS Rhodamine 6G, crystal violet and congo red SERS substrate surface 10 -9 , 10 -8 and 10 -7 M [51] Graphene oxide nanosheet coated with silver and gold nanoparticles Beta-carotene and malachite green Analyte deposited on SERS substrate <1 mg/L [43] Silver nanoparticles -grafted silicon nanocones Rhodamine 6G, crystal violet, melamine, methyl parathion AgNPs/SiNC platform, lake water, milk and tap water 10 -14 , 10 -9 , 10 -7 and 10 -7 M [49] Sulphur doped MoO2 nanospheres Rhodamine 6G, rhodamine B, crystal violet Dropped onto glass slide 1 × 10 -9 , 1 × 10 -10 and 1 × 10 -8 M [266] Silver-doped hydroxyapatite nanocomposite…”