A novel swine model of the acute respiratory distress syndrome using clinically relevant injury exposures

Tiba, Mohamad H.; McCracken, Brendan; Leander, Danielle C; Colmenero, Carmen I; Nemzek, Jean A.; Sjoding, Michael W.; Konopka, Kristine E.; Flott, Thomas L.; VanEpps, J. Scott; Daniels, Rodney C.; Ward, Kevin R.; Stringer, Kathleen A.; Dickson, Robert P.

doi:10.14814/phy2.14871

Cited by 9 publications

(16 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We used a recently described swine model of ARDS that uses clinically relevant exposures (sepsis, hyperoxia, volutrauma and aspiration) and faithfully replicates the physiological, radiographical and histopathological features of ARDS [ 15 ]. As reported recently, this model fulfils both clinical criteria (Berlin definition) [ 9 ] and experimental criteria for animal models of ARDS [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…All procedures were carried out in compliance with the university's institutional standards for care and use of laboratory animals in accordance with National Institutes of Health (NIH) guidelines [ 22 ]. Detailed physiological data regarding these animals have previously been published [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…The anaesthesia level was monitored for the experiment's duration by assessing corneal reflex, jaw tension and haemodynamics, including blood pressure, heart rate and respiratory rate. More details on animal preparation can be found in the studies by Tiba and co-workers [ 15 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…Animals were subjected to systemic infection via an injection of Escherichia coli into the kidney parenchyma (3.5×10 11 CFU Strain CFT073 E. coli in a total of approximately 5 mL at a rate of 0.333 mL·min −1 ), as previously described [ 15 , 23 ]. Lung injury was induced using a combination of volume trauma (tidal volumes of 15 mL·kg −1 ), hyperoxia (100% inspiratory oxygen fraction ( F IO 2 )) and the instillation of gastric particles (1–2 mL·kg −1 ) into the airways via bronchoscope [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our present work, we overcame the aforementioned limitations by using a newly developed and optimised swine model of ARDS that accurately replicates human conditions [ 15 ]. Animal models, particularly large animals such as swine, have been used in biomedical research for decades because of their anatomical and physiological similarities to humans [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Breath analysis for detection and trajectory monitoring of acute respiratory distress syndrome in swine

et al. 2021

Self Cite

View full text Add to dashboard Cite

Despite the enormous impact on human health, acute respiratory distress syndrome (ARDS) is ill-defined, and its timely diagnosis is difficult, as is tracking the course of the syndrome. The objective of this pilot study was to explore the utility of breath collection and analysis methodologies to detect ARDS through changes in the volatile organic compound (VOC) profiles present in breath. Five male Yorkshire mix swine were studied and ARDS was induced utilising both direct and indirect lung injury. An automated portable gas chromatography device developed in-house was used for point of care breath analysis and to monitor swine breath hourly, starting from the initiation of the experiment until the development of ARDS, which was adjudicated based on the Berlin criteria at the breath sampling points and confirmed by lung biopsy at the end of the experiment. A total of 67 breath samples (chromatograms) were collected and analyzed. Through machine learning, principal component analysis, and linear discrimination analysis, seven VOCs biomarkers were identified that distinguished ARDS. These represent seven of the nine biomarkers found in our breath analysis study of human ARDS corroborating our findings. We also demonstrated that breath analysis detects changes 1–6 h earlier than the clinical adjudication based on the Berlin criteria. The findings provide proof of concept that breath analysis can be used for the identification of early changes associated with ARDS pathogenesis in swine. Its clinical application could provide intensive care clinicians with a non-invasive diagnostic tool for early detection and continuous monitoring of ARDS.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Breath analysis for detection and trajectory monitoring of acute respiratory distress syndrome in swine

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Protective effect of genkwanin against lipopolysaccharide-induced acute lung injury in mice with p38 mitogen-activated protein kinase and nuclear factor-κB pathway inhibition

Shen

Chen

et al. 2022

Journal of Functional Foods

View full text Add to dashboard Cite

Bronchoalveolar Lavage and Oleic Acid Two-hit Model for Inducing Acute Respiratory Distress Syndrome in Swine Models

Russo,

Evans,

Sullivan

et al. 2024

Military Medicine

View full text Add to dashboard Cite

Introduction: Acute respiratory distress syndrome (ARDS) is a widespread and often fatal clinical syndrome marked by the acute onset of pulmonary edema and inflammatory-mediated disruptions in alveolar-capillary permeability resulting in impaired gas exchange and tissue oxygenation with subsequent acute respiratory failure that accounts for 10.4% of all intensive care unit admissions worldwide and boasts a mortality rate of 38.5%. The current treatment for ARDS remains largely supportive. This is largely because of the many challenges of achieving a stable and sustainable animal model that recreates the pathophysiology of ARDS experimentally in a controlled setting to allow research to elucidate potential treatments of ARDS moving forward. Materials and Methods The bronchoalveolar lavage and oleic acid models are currently the 2 most frequently used experimental models in inducing ARDS in animal models. This study demonstrated that combining them into a “two-hit model” can produce sustained ARDS in swine models per the Horowitz index (PaO2/FiO2 ratio of ≤300 mmHg). Additionally, expected changes in pH, pCO2, lung compliance, cytokines, and tissue histopathology were observed and add to our confidence and reliability that the “two-hit model” produces symptomatic ARDS in a manner very similar to that observed in humans. Results and Conclusions In conclusion, we demonstrated a viable animal model of human ARDS that is maintained for a prolonged period, suitable for continuous monitoring of the progression, and evaluation of potential future treatments and procedures to reduce patient morbidity and mortality. To carry out this two-hit model, lung injury was induced through a combination of bronchoalveolar lavage and oleic acid administration and the disease process of ARDS is subsequently tracked through clinically relevant parameters such as respiratory mechanics, cytokine response, aretrial blood gas (ABG) changes, and observation of postmortem histopathologic changes. This promising new model has the capacity to successfully replicate human ARDS which is a well-known and notoriously multifactorial pathogenic process to reproduce experimentally for an extended period of time. The “two-hit model” is a viable and appropriate model for the research of novel treatments for ARDS.

show abstract

A novel swine model of the acute respiratory distress syndrome using clinically relevant injury exposures

Cited by 9 publications

References 38 publications

Breath analysis for detection and trajectory monitoring of acute respiratory distress syndrome in swine

Breath analysis for detection and trajectory monitoring of acute respiratory distress syndrome in swine

Protective effect of genkwanin against lipopolysaccharide-induced acute lung injury in mice with p38 mitogen-activated protein kinase and nuclear factor-κB pathway inhibition

Bronchoalveolar Lavage and Oleic Acid Two-hit Model for Inducing Acute Respiratory Distress Syndrome in Swine Models

Contact Info

Product

Resources

About