A Novel System Improves Preservation of Osteochondral Allografts

Cook, James L.; Stoker, Aaron M.; Kuroki, Keiichi; Cook, Cristi R.; Pfeiffer, Ferris M.; Bozynski, Chantelle C.; Hung, Clark T.

doi:10.1007/s11999-014-3773-9

Cited by 95 publications

(131 citation statements)

References 25 publications

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“…While, to our knowledge, no literature currently exists concerning the storage of tissue engineered cartilage grafts, there have been many clinical and basic science studies assessing stored grafts in vivo and in vitro using a variety of storage protocols and evaluation methods. Most commonly, chondrocyte viability is used as the marker of allograft success, yet ideally tissue properties best match freshly harvested tissues, which have been shown to have maximum viability and long‐term implantation success .…”

Section: Discussionmentioning

confidence: 99%

“…Once constructs reached the point of maturity (28–42 days), judged by an average equilibrium compressive Young's Modulus (E Y ) in the native range (> 200 kPa 18 ), constructs were randomly assigned to experimental groups: MOPS‐25, standard preservation in MOPS media at room temperature (25°C); MOPS‐4, preservation in MOPS media with refrigeration (4°C); or continuous culture, in which constructs remained in the culture conditions described above. For constructs in MOPS media, the media were changed once per week and did not contain growth factors or FBS . MOPS media components include DMEM, antibiotics, antimycotics, dexamethasone, ascorbic acid, l ‐proline, sodium pyruvate, insulin, transferrin, and selenous acid.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the limitations of current storage protocols with respect to graft availability, researchers are investigating various methods of storage in an attempt to increase the duration of this storage window . A preservation system recently developed by Stoker et al, called the Missouri Osteochondral Allograft Preservation System (MOPS), has shown maintenance of day 0 chondrocyte viability for at least 63 days without the use of serum, growth factors, or refrigeration . This preservation system reduces concerns associated with xenogenic media components and simplifies storage logistics.…”

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confidence: 99%

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Long‐term storage and preservation of tissue engineered articular cartilage

Nover

Stefani

Lee

et al. 2015

Journal Orthopaedic Research

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With limited availability of osteochondral allografts, tissue engineered cartilage grafts may provide an alternative treatment for large cartilage defects. An effective storage protocol will be critical for translating this technology to clinical use. The purpose of this study was to evaluate the efficacy of the Missouri Osteochondral Allograft Preservation System (MOPS) for room temperature storage of mature tissue engineered grafts, focusing on tissue property maintenance during the current allograft storage window (28 days). Additional research compares MOPS to continued culture, investigates temperature influence, and examines longer-term storage. Articular cartilage constructs were cultured to maturity using adult canine chondrocytes, then preserved with MOPS at room temperature, in refrigeration, or kept in culture for an additional 56 days. MOPS storage maintained desired chondrocyte viability for 28 days of room temperature storage, retaining 75% of the maturity point Young's modulus without significant decline in biochemical content. Properties dropped past this time point. Refrigeration maintained properties similar to room temperature at 28 days, but proved better at 56 days. For engineered grafts, MOPS maintained the majority of tissue properties for the 28-day window without clearly extending that period as it had for native grafts. These results are the first evaluating engineered cartilage storage.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Long‐term storage and preservation of tissue engineered articular cartilage

Nover

Stefani

Lee

et al. 2015

Journal Orthopaedic Research

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“…2014). This proprietary tissue cryopreservation technique differs from past attempts: all bone, apart from a few microns, is removed and the resulting cartilage is then perforated.…”

Section: Methodsmentioning

confidence: 99%

Cartilage repair in the degenerative ageing knee

et al. 2016

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Background and purposeCartilage damage can develop due to trauma, resulting in focal chondral or osteochondral defects, or as more diffuse loss of cartilage in a generalized organ disease such as osteoarthritis. A loss of cartilage function and quality is also seen with increasing age. There is a spectrum of diseases ranging from focal cartilage defects with healthy surrounding cartilage to focal lesions in degenerative cartilage, to multiple and diffuse lesions in osteoarthritic cartilage. At the recent Aarhus Regenerative Orthopaedics Symposium (AROS) 2015, regenerative challenges in an ageing population were discussed by clinicians and basic scientists. A group of clinicians was given the task of discussing the role of tissue engineering in the treatment of degenerative cartilage lesions in ageing patients. We present the outcomes of our discussions on current treatment options for such lesions, with particular emphasis on different biological repair techniques and their supporting level of evidence.Results and interpretationBased on the studies on treatment of degenerative lesions and early OA, there is low-level evidence to suggest that cartilage repair is a possible treatment for such lesions, but there are conflicting results regarding the effect of advanced age on the outcome. We concluded that further improvements are needed for direct repair of focal, purely traumatic defects before we can routinely use such repair techniques for the more challenging degenerative lesions. Furthermore, we need to identify trigger mechanisms that start generalized loss of cartilage matrix, and induce subchondral bone changes and concomitant synovial pathology, to maximize our treatment methods for biological repair in degenerative ageing joints.

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“…Procedures that provide immediate restoration of tissue biomechanics in the defect—with recapitulation of the functional articular cartilage–subchondral bone unit and re‐establishment of tissue composition, zonal architecture and material properties—provide faster rehabilitation when compared to cell‐based, cartilage‐only techniques (with or without scaffolds). Current technologies that possess these capabilities include osteochondral autografts and osteochondral allografts . Technologies that may fulfill these criteria in the foreseeable future include tissue engineered osteochondral constructs …”

mentioning

confidence: 99%