A novel technique for the separation and concentration of penicillin G from fermentation broth: Aqueous two-phase flotation

Bi, Pengyu; Li, Dianqing; Dong, Hao

doi:10.1016/j.seppur.2009.07.019

Cited by 85 publications

(33 citation statements)

References 39 publications

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“…Subsequently, (NH 4 ) 2 SO 4 was added into the combined extracts, and the aqueous solution was used for the separation and concentration of ATPF. To accurately and conveniently prepare the aqueous (NH 4 ) 2 SO 4 solution, the classical relationship equation between the volume concentration and the mass concentration of (NH 4 ) 2 SO 4 was applied. Moreover, the PEG phase containing 50 wt% water (or ethanol) was used to collect the target compounds on the top of flotation column.…”

Section: Methodsmentioning

confidence: 99%

Separation of four flavonol glycosides from Solanum rostratum Dunal using aqueous two‐phase flotation followed by preparative high‐performance liquid chromatography

Lin

Shao

et al. 2016

J of Separation Science

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Aqueous two‐phase flotation followed by preparative high‐performance liquid chromatography was used to separate four flavonol glycosides from Solanum rostratum Dunal. In the aqueous two‐phase flotation section, the effects of sublation solvent, solution pH, (NH4)2SO4 concentration in aqueous solution, cosolvent, N2 flow rate, flotation time, and volumes of the polyethylene glycol phase on the recovery were investigated in detail, and the optimal conditions were selected: 50 wt% polyethylene glycol 1000 ethanol solvent as the flotation solvent, pH 4, 350 g/L of (NH4)2SO4 concentration in aqueous phase, 40 mL/min of N2 flow rate, 30 min of flotation time, 10.0 mL of flotation solvent volume, and two times. After aqueous two‐phase flotation concentration, the flotation products were purified by preparative high‐performance liquid chromatography. The purities of the final products A and B were 98.1 and 99.0%. Product B was the mixture of three compounds based on the analysis of high‐performance liquid chromatography at the temperature of 10°C, while product A was hyperoside after the identification by nuclear magnetic resonance. Astragalin, 3’‐O‐methylquercetin 3‐O‐β‐d‐galactopyranoside, and 3’‐O‐methylquercetin 3‐O‐β‐d‐glucopyranoside were obtained with the purity of 93.8, 97.1, and 99.2%, respectively, after the further separation of product B using preparative high‐performance liquid chromatography.

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Section: Methodsmentioning

confidence: 99%

Separation of four flavonol glycosides from Solanum rostratum Dunal using aqueous two‐phase flotation followed by preparative high‐performance liquid chromatography

Lin

Shao

et al. 2016

J of Separation Science

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“…Aqueous two‐phase flotation (ATPF) is a novel recovery method which integrates ATPS with solvent sublation (SS) [51]. SS is an adsorptive bubble separation technique which works by bubbling nitrogen gas through the bottom enzyme‐rich solution, capturing surface‐active (or hydrophobic) compounds by adsorption onto the nitrogen gas, and the ascending bubbles then dissolve in the top polymer phase.…”

Section: Novel Lipase Purification Methodsmentioning

confidence: 99%

Novel lipase purification methods – a review of the latest developments

Tan

Show

Ooi

et al. 2014

Biotechnology Journal

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Microbial lipases are popular biocatalysts due to their ability to catalyse diverse reactions such as hydrolysis, esterification, and acidolysis. Lipases function efficiently on various substrates in aqueous and non-aqueous media. Lipases are chemo-, regio-, and enantio-specific, and are useful in various industries, including those manufacturing food, detergents, and pharmaceuticals. A large number of lipases from fungal and bacterial sources have been isolated and purified to homogeneity. This success is attributed to the development of both conventional and novel purification techniques. This review highlights the use of these techniques in lipase purification, including conventional techniques such as: (i) ammonium sulphate fractionation; (ii) ion-exchange; (iii) gel filtration and affinity chromatography; as well as novel techniques such as (iv) reverse micellar system; (v) membrane processes; (vi) immunopurification; (vi) aqueous two-phase system; and (vii) aqueous two-phase floatation. A summary of the purification schemes for various bacterial and fungal lipases are also provided.

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“…2;Zaslavsky, 1995, chap. 3;Tan et al, 2013) is more widely used to separate and extract the biological materials, such as nucleic acids (Luechau, Ling, & Lyddiatt, 2009), proteins (Rawdkuen, Pintathong, Chaiwut, & Benjakul, 2011;Ooi, Hii, Kamal, Ariff, & Ling, 2011;Yücekan & Önal, 2011), viruses (Luechau, Ling, & Lyddiatt, 2011), antibiotics Bi, Li, & Dong, 2009;Xie, Wang, Han, & Yan, 2011;Chen et al, 2014) and other biological molecules (Silva, Coimbra, Rojas, & Teixeira, 2009;Azevedo et al, 2009;Gomes, Azevedo, Aires-Barros, & Prazeres, 2009). Nowadays, the ATPSs mainly divides into the following four kinds: the polymer-polymer ATPSs (Li & Cao, 2010), polymer-salt ATPSs (Zafarani-Moattar & Hosseinpour-Hashemi, 2012), ion liquid-salt ATPSs (Han et al, 2012) and micromolecule alcohol-salt ATPSs Zafarani-Moattar, Nemati-Kande, & Soleimani, 2012).…”

Section: Introductionmentioning

confidence: 99%

Separation, concentration and determination of trace chloramphenicol in shrimp from different waters by using polyoxyethylene lauryl ether-salt aqueous two-phase system coupled with high-performance liquid chromatography

Yao

et al. 2016

Food Chemistry

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A novel technique for the separation and concentration of penicillin G from fermentation broth: Aqueous two-phase flotation

Cited by 85 publications

References 39 publications

Separation of four flavonol glycosides from Solanum rostratum Dunal using aqueous two‐phase flotation followed by preparative high‐performance liquid chromatography

Separation of four flavonol glycosides from Solanum rostratum Dunal using aqueous two‐phase flotation followed by preparative high‐performance liquid chromatography

Novel lipase purification methods – a review of the latest developments

Separation, concentration and determination of trace chloramphenicol in shrimp from different waters by using polyoxyethylene lauryl ether-salt aqueous two-phase system coupled with high-performance liquid chromatography

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