A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents

Romdhane, Ines Belhaj-Ben; Fendri, Ahmed; Gargouri, Youssef; Gargouri, Ali; Belghith, Hafedh

doi:10.1016/j.bej.2010.10.002

Cited by 97 publications

(61 citation statements)

References 21 publications

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“…4). This observation is similar to the findings reported earlier for CRL immobilized onto styrene divinylbenzene copolymer [36] as well as Talaromyces thermophilus lipase immobilized onto chitosan [37]. Adsorption of the free CRL onto the acid-functionalized MWCNTs may have altered the physical and chemical properties of the free CRL.…”

Section: Effect Of Temperaturesupporting

confidence: 89%

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

Marzuki

Mahat

Huyop

et al. 2015

Appl Biochem Biotechnol

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The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via environmentally favorable processes is of concern. In this context, an alternative technique utilizing Candida rugosa lipase (CRL) physically adsorbed on multi-walled carbon nanotubes (MWCNTs) has been suggested. In this study, the acid-functionalized MWCNTs prepared using a mixture of HNO3 and H2SO4 (1:3 v/v) was used as support for immobilizing CRL onto MWCNTs (CRL-MWCNTs) as biocatalysts. Enzymatic esterification was performed and the efficiency of CRL-MWCNTs was evaluated against the free CRL under varying conditions, viz. temperature, molar ratio of acid/alcohol, solvent log P, and enzyme loading. The CRL-MWCNTs resulted in 30-110 % improvement in the production of methyl oleate over the free CRL. The CRL-MWCNTs attained its highest yield (84.17 %) at 50 °C, molar ratio of acid/alcohol of 1:3, 3 mg/mL of enzyme loading, and iso-octane (log P 4.5) as solvent. Consequently, physical adsorption of CRL onto acid-functionalized MWCNTs has improved the activity and stability of CRL and hence provides an environmentally friendly means for the production of methyl oleate.

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Section: Effect Of Temperaturesupporting

confidence: 89%

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

Marzuki

Mahat

Huyop

et al. 2015

Appl Biochem Biotechnol

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“…Since it is a byproduct of the milling of wheat into white flour, WB usually accounts for 14-19% of the grains' weight (Maes and Delcour, 2002;Demir and Tari, 2014). This residue is also an inexpensive carbon source that has already been reported as a good inductor for lipase activity (Romdhane et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

NITROGEN SOURCES ON TPOMW VALORIZATION THROUGH SOLID STATE FERMENTATION PERFORMED BY Yarrowia lipolytica

Lopes

Farias

Belo

et al. 2016

Braz. J. Chem. Eng.

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-This manuscript reports the valorization of two-phase olive mill waste (TPOMW) as raw material and carbon source for solid state fermentation using Yarrowia lipolytica as biocatalyst. Due to its chemical characteristics, a combination of different raw materials (TPOMW and wheat bran, WB) was evaluated and two distinct nitrogen sources were applied as supplementation for lipase production. A TPOMW/WB ratio of 1:1 and supplementation with ammonium sulfate was chosen as the best condition. The productivity in 24 h reached 7.8 U/g*h and, after four days of process, only decreased about 35%. Process pH ranged from 5.5 -5.9, remaining in an acid range. Thus, the successful use of TPOMW, a watery solid by-product with high content of lipids, as raw material for Yarrowia lipolytica growth and lipase production provided an environmental friendly alternative to valorize such waste.

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“…The main commercial lipase preparation is from Aspergillus oryzae, created from lipase clones derived from Thermomyces lanuginosa (Lipolase from Novo Nordisk) and lipase clones from Rhizomucor miehei (Lipozyme, Novo Nordisk S/A). They are especially applied as detergents and production of analogues of cocoa butter from cheap oil sources (Romdhane et al 2010). …”

Section: Lipasesmentioning

confidence: 99%

Exploration of Microorganisms Producing Bioactive Molecules of Industrial Interest by Solid State Fermentation

Fleuri

Kawaguti

Pedrosa

et al. 2013

Food Quality, Safety and Technology

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The prospect of biomolecules using microorganisms in fermentation processes is widely used, in this context to solid state fermentation (SSF) has advantages such as the possibility of using agricultural and industrial waste and reduction of water waste. Studies show that different microorganisms can be used in SSF; actinomyces and fungi are the most used due to growth in media with low water activity. Among the highlight biomolecules produced are antibiotics, anticarcinogenic agents, anticoccidians, antiviral, neuroactive, antioxidants, and enzymes. The enzymes are produced in greater scale among the different classes; hydrolases have gained importance because of cellulases, hemicellulases, proteases, chitinases, lipases, and phytases. Cellulases are a complex capable of acting on cellulosic materials, promoting its hydrolysis to release sugars, of which glucose is the one with largest industrial interest. Xylanolytic enzymes act on xylan, hemicellulose components, which may be attached to the cellulose and lignin in the plant cell wall. The study of chitinase has been stimulated by their possible involvement as agents of defense against pathogenic organisms that contain chitin,

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A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents

Cited by 97 publications

References 21 publications

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

Candida rugosa Lipase Immobilized onto Acid-Functionalized Multi-walled Carbon Nanotubes for Sustainable Production of Methyl Oleate

NITROGEN SOURCES ON TPOMW VALORIZATION THROUGH SOLID STATE FERMENTATION PERFORMED BY Yarrowia lipolytica

Exploration of Microorganisms Producing Bioactive Molecules of Industrial Interest by Solid State Fermentation

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