A Novel Thioredoxin-Dependent Peroxiredoxin (TPx-Q) Plays an Important Role in Defense Against Oxidative Stress and Is a Possible Drug Target in Babesia microti

Zhang, Houshuang; Wang, Zhonghua; Huang, Jingsheng; Cao, Jie; Zhou, Yongzhi; Zhou, Jinlin

doi:10.3389/fvets.2020.00076

Cited by 14 publications

(6 citation statements)

References 62 publications

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“…It was suggested that peptidylprolyl isomerases have a role in abiotic stress response in plants 69 . In addition, thioredoxin-dependent peroxiredoxin as part of antioxidant defenses and redox signaling is essential for cellular response against oxidative stress 70 . Previous observation implies a possible link between mitochondrial thioredoxin system and peptidyl prolyl cis –trans isomerase activity 71 .…”

Section: Discussionmentioning

confidence: 99%

Integration of meta-analysis, machine learning and systems biology approach for investigating the transcriptomic response to drought stress in Populus species

Tahmasebi

Niazi‎

Akrami

2023

Sci Rep

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In Populus, drought is a major problem affecting plant growth and development which can be closely reflected by corresponding transcriptomic changes. Nevertheless, how these changes in Populus are not fully understood. Here, we first used meta-analysis and machine learning methods to identify water stress-responsive genes and then performed a systematic approach to discover important gene networks. Our analysis revealed that large transcriptional variations occur during drought stress. These changes were more associated with the response to stress, cellular catabolic process, metabolic pathways, and hormone-related genes. The differential gene coexpression analysis highlighted two acetyltransferase NATA1-like and putative cytochrome P450 genes that have a special contribution in response to drought stress. In particular, the findings showed that MYBs and MAPKs have a prominent role in the drought stress response that could be considered to improve the drought tolerance of Populus. We also suggest ARF2-like and PYL4-like genes as potential markers for use in breeding programs. This study provides a better understanding of how Populus responses to drought that could be useful for improving tolerance to stress in Populus.

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Section: Discussionmentioning

confidence: 99%

Integration of meta-analysis, machine learning and systems biology approach for investigating the transcriptomic response to drought stress in Populus species

Tahmasebi

Niazi‎

Akrami

2023

Sci Rep

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“…Despite the different proteins identified, thioredoxin-dependent peroxidoxin and cysteine protease with granulin and papain domain are enzymes that have a cysteine residue in their active site. The first protein is a peroxidase related to antioxidant defense, since it reduces hydrogen peroxide (H 2 O 2 ) using a peroxidatic cysteine, protecting cells against the toxicity of reactive oxygen species (ROS) and reactive nitrogen species (RNS) ( Knoops et al., 2016 ; Zhang et al., 2020 ). The second protein is a protease containing granulin domain and has been implicated in plant cell death mechanisms under stress ( Shindo et al., 2012 ; Lampl et al., 2013 ; Rustgi et al., 2017 ; Lema Asqui et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens

Ferreira,

Farias,

Zugaib

et al. 2024

Front. Plant Sci.

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In plants, serpins are a superfamily of serine and cysteine protease inhibitors involved in stress and defense mechanisms, with potential for controlling agricultural pests, making them important biotechnological tools. The objective of this study was to characterize a serpin from Theobroma cacao, called TcSERPIN, to identify its endogenous targets and determine its function and biotechnological potential. TcSERPIN has 390 amino acid residues and shows conservation of the main active site, RCL. Cis-elements related to light, stress, hormones, anaerobic induction, cell cycle regulation and defense have been identified in the gene’s regulatory region. TcSERPIN transcripts are accumulated in different tissues of Theobroma cacao. Furthermore, in plants infected with Moniliophtora perniciosa and Phytophthora palmivora, the expression of TcSERPIN was positively regulated. The protein spectrum, rTcSERPIN, reveals a typical β-sheet pattern and is thermostable at pH 8, but loses its structure with temperature increases above 66°C at pH 7. At the molar ratios of 0.65 and 0.49, rTcSERPIN inhibited 55 and 28% of the activity of papain from Carica papaya and trypsin from Sus scrofa, respectively. The protease trap containing immobilized rTcSERPIN captured endogenous defense proteins from cocoa extracts that are related to metabolic pathways, stress and defense. The evaluation of the biotechnological potential against geohelminth larvae showed that rTcSERPIN and rTcCYS4 (Theobroma cacao cystatin 4) reduced the movement of larvae after 24 hours. The results of this work show that TcSERPIN has ideal biochemical characteristics for biotechnological applications, as well as potential for studies of resistance to phytopathogens of agricultural crops.

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“…As the O 2 diffuses in all directions the parasite lives in the line of fire. During the brief bouts of high O 2 partial pressure oxidative damage may occur to many components, perhaps exacerbated by hemoglobin peroxidase activity and associated radicals ( Reeder, 2017 ; Zhang et al., 2020 ). It is clear that appreciable oxidative damage occurs, based upon the accumulation of the lipid oxidation product, malondialdehyde, in IE membranes and fragmentation of DNA ( Commins et al., 1988 ; Murase et al., 1996 ; Esmaeilnejad et al., 2020 ), challenging parasite genome integrity.…”

Section: Why Cytoadhesion?mentioning

confidence: 99%

“…Malondialdehyde is itself carcinogenic, forming DNA base adducts ( Marnett, 2000 ). Damage generated by reactive oxygen species or nitrosylation from innate immune responses also would contribute to selective pressure ( Asada et al., 2015 ; Zhang et al., 2020 ). Cytoadhesive parasites avoid both clearance by the spleen and genotoxic damage from O 2 acquired in the lungs.…”

Section: Why Cytoadhesion?mentioning

confidence: 99%

Integration of DNA Repair, Antigenic Variation, Cytoadhesion, and Chance in Babesia Survival: A Perspective

Allred

2022

Front. Cell. Infect. Microbiol.

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Apicomplexan parasites live in hostile environments in which they are challenged chemically and their hosts attempt in many ways to kill them. In response, the parasites have evolved multiple mechanisms that take advantage of these challenges to enhance their survival. Perhaps the most impressive example is the evolutionary co-option of DNA repair mechanisms by the parasites as a means to rapidly manipulate the structure, antigenicity, and expression of the products of specific multigene families. The purpose of variant proteins that mediate cytoadhesion has long been thought to be primarily the avoidance of splenic clearance. Based upon known biology, I present an alternative perspective in which it is survival of the oxidative environment within which Babesia spp. parasites live that has driven integration of DNA repair, antigenic variation, and cytoadhesion, and speculate on how genome organization affects that integration. This perspective has ramifications for the development of parasite control strategies.

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A Novel Thioredoxin-Dependent Peroxiredoxin (TPx-Q) Plays an Important Role in Defense Against Oxidative Stress and Is a Possible Drug Target in Babesia microti

Cited by 14 publications

References 62 publications

Integration of meta-analysis, machine learning and systems biology approach for investigating the transcriptomic response to drought stress in Populus species

Integration of meta-analysis, machine learning and systems biology approach for investigating the transcriptomic response to drought stress in Populus species

TcSERPIN, an inhibitor that interacts with cocoa defense proteins and has biotechnological potential against human pathogens

Integration of DNA Repair, Antigenic Variation, Cytoadhesion, and Chance in Babesia Survival: A Perspective

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