A novel three-dimensional scaffold for regenerative endodontics: materials and biological characterizations

Bottino, Marco C.; Yassen, Ghaeth H.; Platt, Jeffrey A.; Labban, Nawaf; Windsor, L. Jack; Spolnik, Kenneth J.; Bressiani, Ana Helena A.

doi:10.1002/term.1712

Cited by 86 publications

(86 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another approach to deliver these low antibiotic concentrations into the root canal could be through the use of a three-dimensional scaffold system. Recent studies have proposed the use of a bio-resorbable antibiotic-loaded scaffold to disinfect the root canal space in endodontic regeneration [38,39] …”

Section: Discussionmentioning

confidence: 99%

The effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established Enterococcus faecalis biofilm

et al. 2015

Self Cite

View full text Add to dashboard Cite

Materials and Methods:The cytotoxic and antibacterial effects of different triple (TAP) and double antibiotic (DAP) paste dilutions (0.125, 0.25, 0.5, 1, and 10 mg/ml) were tested against Enterococcus faecalis established biofilm and DPSC. Established bacterial biofilm were exposed to antibiotic dilutions for 3 days. Then, biofilms were collected, spiral plated and the numbers of bacterial colony forming units (CFU/mL) were determined. For the cytotoxic effect, lactate dehydrogenase activity assays (LDH) and cell viability assays (WST-1) were used to measure the percentage of DPSC cytotoxicity after 3 day treatment with the same antibiotic dilutions. A general linear mixed model was used for statistical analyses (α=0.05).Results: All antibiotic dilutions significantly decreased the bacterial CFU/mL. For WST-1 assays, all antibiotic dilutions except 0.125 mg/ml significantly reduced the viability of DPSC. For LDH assays, the three lowest tested concentrations of DAP (0.5, 0.25, 0.125 mg/ml) and the two lowest concentrations of TAP (0.25 and 0.125 mg/ml) were non-toxic to DPSC. Conclusions: All tested dilutions had an antibacterial effect against Enterococcus faecalis.However, 0.125 mg/ml of DAP and TAP showed a significant antibacterial effect with no cytotoxic effects on DPSC.Clinical relevance: Using appropriate antibiotic concentrations of intracanal medicament during endodontic regeneration procedures is critical to disinfect root canal and decrease the adverse effects on stem cells. KEYWORDSTriple antibiotic paste, double antibiotic paste, Enterococcus faecalis, established biofilm, dental pulp stem cells.

show abstract

Section: Discussionmentioning

confidence: 99%

The effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established Enterococcus faecalis biofilm

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fiber diameter was determined from the SEM images using ImageJ (1.44i, National Institutes of Health, USA). The tensile strength of the scaffolds (15 × 3 mm 2 , n=6/group), was evaluated by uni-axial tensile testing (expert 5601, ADMET, Norwood, MA) (13, 14). To confirm antibiotic incorporation, Fourier transform infrared spectroscopy (FTIR, Jasco 4100, Easton, MD) using attenuated total reflection (ATR) was conducted (13).…”

Section: Methodsmentioning

confidence: 99%

Bimix Antimicrobial Scaffolds for Regenerative Endodontics

Palasuk

Kamocki

Hippenmeyer

et al. 2014

Journal of Endodontics

Self Cite

View full text Add to dashboard Cite

Introduction Eliminating and/or inhibiting bacterial growth within the root canal system have been shown to play a key role in the regenerative outcome. The aim of this study was to synthesize and determine in vitro both the antimicrobial effectiveness and cytocompatibility of bi-mix antibiotic-containing polydioxanone (PDS)-based polymer scaffolds. Methods Antibiotic-containing (metronidazole, MET and ciprofloxacin, CIP) polymer solutions (distinct antibiotic weight ratios) were spun into fibers as a potential mimic to the double antibiotic paste (DAP, a MET/CIP mixture). Fiber morphology, chemical characteristics, and tensile strength were evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and tensile testing, respectively. Antimicrobial efficacy was tested over time (aliquot collection) against Enterococcus faecalis (Ef), Porphyromonas gingivalis (Pg), and Fusobacterium nucleatum (Fn). Similarly, cytotoxicity was evaluated in human dental pulp stem cells (hDPSCs). Data were statistically analyzed (p<0.05). Results SEM and FTIR confirmed that electrospinning was able to produce antibiotic-containing fibers with diameter mostly in the nanoscale. Tensile strength of 1:1MET/CIP scaffolds was significantly (p<0.05) higher than pure PDS (control). Meanwhile, all other groups presented similar strength as the control. Aliquots obtained from antibiotic-containing scaffolds inhibited growth of Ef, Pg and Fn, except pure MET that did not show an inhibitory action towards Pg or Fn. Antibiotic-containing aliquots promoted slight hDPSCs viability reduction, but none of them were considered to be cytotoxic. Conclusion Our data suggest that the incorporation of multiple antibiotics within a nanofibrous scaffold holds great potential towards the development of a drug delivery system for regenerative endodontics.

show abstract

“…1B) (Bottino et al, 2014b). Briefly, biodegradable polymer nanocomposite fibrous scaffolds were synthesized with aluminosilicate clay nanotubes to serve as a delivery vehicle of bioactive agents (e.g., antimicrobial drugs and angiogenic factors, among others) for controlled release within the root canal system.…”

Section: Bioactive Scaffolds For Pulp-dentin Complex Regenerationmentioning

confidence: 99%

Tissue-engineering-based Strategies for Regenerative Endodontics

et al. 2014

View full text Add to dashboard Cite

Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo ( i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.

show abstract

A novel three-dimensional scaffold for regenerative endodontics: materials and biological characterizations

Cited by 86 publications

References 44 publications

The effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established Enterococcus faecalis biofilm

The effect of diluted triple and double antibiotic pastes on dental pulp stem cells and established Enterococcus faecalis biofilm

Bimix Antimicrobial Scaffolds for Regenerative Endodontics

Tissue-engineering-based Strategies for Regenerative Endodontics

Contact Info

Product

Resources

About