A Novel Tissue Culture Tray for the Study of Magnetically Induced Rotation and Translation of Iron Oxide Nanoparticles

Engelhard, Herbert H.; Pernal, Sebastian; Gaertner, Zachary; Levin, Adam; Pan, Yayue; Morris, Sean C.; Sabo, Michael; Creighton, F.M.

doi:10.1109/lmag.2017.2761818

Cited by 14 publications

(30 citation statements)

References 26 publications

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“…61 Yet to date, only a few studies have analyzed the effect of these factors on MNP movement, aggregation/agglomeration, and/or drug delivery. 29,62 Critically, when nanoparticles enter a physiologic environment, they rapidly adsorb proteins and form a protein corona, which imparts a biological identity that is distinct from their synthetic identity. 59,63,64 The drug delivery principle seen in our studies -that of moving unbound drug with MNP clustersmay essentially be a collective corona effect.…”

Section: Discussionmentioning

confidence: 99%

“…Characterization of these MNPs, including their hysteresis curve and TEM imaging, has recently been reported. 29 The volume of MNP suspension used in most experiments described here was 20 µL. This was from a stock solution of 25 mg/mL, so typically 0.5 mg of MNPs was used for each determination (i.e., per lane as described below).…”

Section: Magnetic Nanoparticle Microbeads and The Mini-med Systemmentioning

confidence: 99%

“…This has primarily been due to the problem of scale since the attractive force between MNPs and a magnet is inversely proportional to at least the fourth power of the distance. 29,30 Recently, however, our group reported the development of a model system which allows MNPs to be moved "remotely," over human-sized distances (e.g., 5-30 cm) by means of a rotating permanent magnet. 29,31,32 This system employs macroscopic lanes which may be coated with endothelial cells, thereby simulating blood vessels, and allows videography for quantification of the velocities of the MNP clusters moving down the lanes.…”

Section: Introductionmentioning

confidence: 99%

“…29,30 Recently, however, our group reported the development of a model system which allows MNPs to be moved "remotely," over human-sized distances (e.g., 5-30 cm) by means of a rotating permanent magnet. 29,31,32 This system employs macroscopic lanes which may be coated with endothelial cells, thereby simulating blood vessels, and allows videography for quantification of the velocities of the MNP clusters moving down the lanes. This "remote" induction of MNP movement over such distances has been referred to as "magnetically-enhanced diffusion" or "magneticallyinduced rotation and translation (MIRT)".…”

Section: Introductionmentioning

confidence: 99%

“…This "remote" induction of MNP movement over such distances has been referred to as "magnetically-enhanced diffusion" or "magneticallyinduced rotation and translation (MIRT)". 29,33,34 In this paper, we report on the next phase of testing of this system, for measuring MNP-mediated delivery of tPA to blood clots in vitro. Our hypothesis was that rotating MNP clusters could be used to enhance delivery of tPA to a clot in a biomimetic channel, even without direct drug conjugation to the MNPs.…”

Section: Introductionmentioning

confidence: 99%

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<p>An in vitro Model System for Evaluating Remote Magnetic Nanoparticle Movement and Fibrinolysis</p>

Pernal¹,

Willis²,

Sabo³

et al. 2020

IJN

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Background: Thrombotic events continue to be a major cause of morbidity and mortality worldwide. Tissue plasminogen activator (tPA) is used for the treatment of acute ischemic stroke and other thrombotic disorders. Use of tPA is limited by its narrow therapeutic time window, hemorrhagic complications, and insufficient delivery to the location of the thrombus. Magnetic nanoparticles (MNPs) have been proposed for targeting tPA delivery. It would be advantageous to develop an improved in vitro model of clot formation, to screen thrombolytic therapies that could be enhanced by addition of MNPs, and to test magnetic drug targeting at human-sized distances. Methods: We utilized commercially available blood and endothelial cells to construct 1/8th inch (and larger) biomimetic vascular channels in acrylic trays. MNP clusters were moved at a distance by a rotating permanent magnet and moved along the channels by surface walking. The effect of different transport media on MNP velocity was studied using video photography. MNPs with and without tPA were analyzed to determine their velocities in the channels, and their fibrinolytic effect in wells and the trays. Results: MNP clusters could be moved through fluids including blood, at human-sized distances, down straight or branched channels, using the rotating permanent magnet. The greatest MNP velocity was closest to the magnet: 0.76 ± 0.03 cm/sec. In serum, the average MNP velocity was 0.10 ± 0.02 cm/sec. MNPs were found to enhance tPA delivery, and cause fibrinolysis in both static and dynamic studies. Fibrinolysis was observed to occur in 85% of the dynamic MNP + tPA experiments. Conclusion: MNPs hold great promise for use in augmenting delivery of tPA for the treatment of stroke and other thrombotic conditions. This model system facilitates side by side comparisons of MNP-facilitated drug delivery, at a human scale.

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Section: Discussionmentioning

confidence: 99%

Section: Magnetic Nanoparticle Microbeads and The Mini-med Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

<p>An in vitro Model System for Evaluating Remote Magnetic Nanoparticle Movement and Fibrinolysis</p>

Pernal¹,

Willis²,

Sabo³

et al. 2020

IJN

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Mono and Multi‐Stimuli Responsive Polymers: Application as Intelligent Nano‐Drug Delivery Systems

Patil

Gadad

Dandagi

2021

Nanopharmaceutical Advanced Delivery Systems

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Trends in Micro‐/Nanorobotics: Materials Development, Actuation, Localization, and System Integration for Biomedical Applications

et al. 2020

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Micro‐/nanorobots (m‐bots) have attracted significant interest due to their suitability for applications in biomedical engineering and environmental remediation. Particularly, their applications in in vivo diagnosis and intervention have been the focus of extensive research in recent years with various clinical imaging techniques being applied for localization and tracking. The successful integration of well‐designed m‐bots with surface functionalization, remote actuation systems, and imaging techniques becomes the crucial step toward biomedical applications, especially for the in vivo uses. This review thus addresses four different aspects of biomedical m‐bots: design/fabrication, functionalization, actuation, and localization. The biomedical applications of the m‐bots in diagnosis, sensing, microsurgery, targeted drug/cell delivery, thrombus ablation, and wound healing are reviewed from these viewpoints. The developed biomedical m‐bot systems are comprehensively compared and evaluated based on their characteristics. The current challenges and the directions of future research in this field are summarized.

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A Novel Tissue Culture Tray for the Study of Magnetically Induced Rotation and Translation of Iron Oxide Nanoparticles

Cited by 14 publications

References 26 publications

<p>An in vitro Model System for Evaluating Remote Magnetic Nanoparticle Movement and Fibrinolysis</p>

<p>An in vitro Model System for Evaluating Remote Magnetic Nanoparticle Movement and Fibrinolysis</p>

Mono and Multi‐Stimuli Responsive Polymers: Application as Intelligent Nano‐Drug Delivery Systems

Trends in Micro‐/Nanorobotics: Materials Development, Actuation, Localization, and System Integration for Biomedical Applications

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