A novel toll-like receptor from the pearl oyster Pinctada fucata martensii is induced in response to stress

Wu, Yuyuan; Liang, Haiying; Wang, Zhixin; Lei, Qiannan; Xu, Liqun

doi:10.1016/j.cbpb.2017.08.006

Cited by 20 publications

(7 citation statements)

References 51 publications

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“…They play a key role in early host defense against invading pathogens by recognizing conserved PAMPs and activating downstream signaling pathways which can induce the production of in ammatory cytokines and/or type I interferon to clear invading pathogens. Along with the TLRs identi ed in this study, TLRs have also been reported in C.gigas genome [10] and other molluscs such as the pearl oyster Pinctada fucata martensii [39], Hyriopsis cumingii [40]. Wang et al reported that TLR6, identi ed in C.gigas, could function as an important pattern recognition receptor in the early detection and response against invading gram-negative bacteria V. anguillarum, V. splendidus, and grampositive bacteria Staphylococci aureus, Micrococcus luteus, and fungi Pichia pastoris in oysters [41].…”

Section: Toll-like Receptorssupporting

confidence: 65%

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Fang¹,

Cai²,

Mao³

et al. 2020

Preprint

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Background: Marine molluscs living in intertidal and estuarine areas, such as oysters, face numerous pathogen challenges during their development. Infection from bacteria such as Vibrio alginolyticus, represents a major factor affecting larval development and frequently leads to high mortality of the pacific oyster, Crassostrea gigas. The oyster immune response is known to play an important role in protecting the animal during development by mitigating the consequences of infection. Results: In this study, we undertook a comprehensive analysis of the immune response of C. gigas to V. alginolyticus challenge. We sequenced the transcriptome of C. gigas at 0, 6, 12, 24, 48 and 72 hours post infection. After RNA-seq, the raw reads are available through the NCBI Sequence Read Archive under accession number PRJNA623063. After filtering, a total of 58.24 Gb clean reads were produced and assembled using the reference genome of C. gigas. The distribution of quality Q30 was higher than 90.88% for each sample and the GC content ranged from 41.27% to 42.91%. When compared with sequences in the COG, GO, KEGG, Swiss-Prot, and NR databases, there were 1267, 1112, 2187, 682, 1133 differentially expressed genes annotated at 6, 12, 24, 48, 72 hours post infection respectively. Numerous immune-related genes displayed differential expression that varied over time: toll-like receptors, tripartite motif proteins, Lectin-like factors, scavenger receptors, signaling pathway components such as Myeloid differentiation factor 88, and stress proteins such as Heat shock 70 kDa protein were all found to be higher in abundance following V. alginolyticus challenge compared to control. For analysis, these genes were divided into several categories such as pattern recognition receptors, fibrinogen-like proteins, damage associated molecular patterns, complement factors, etc. These general categories allowed us to generate an immune response profile for C. gigas over the first 72 hours of infection. These results indicate that bacterial infection induce a complex pattern of immune gene expression in C. gigas larvae. Conclusion: Our study will facilitate targeted investigation into the function of specific immune factors that may explain the diversity and evolution of invertebrate immune molecules and lead to the development of effective measures to improve the performance of oyster culture.

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Section: Toll-like Receptorssupporting

confidence: 65%

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Fang¹,

Cai²,

Mao³

et al. 2020

Preprint

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“…So -TLR γ, previously identified in ANG, contains one signal peptide, eleven LRRs (leucine rich repeats), one transmembrane domain, and the TIR (Toll interleukin-1 resistance) domain. So -TLRγ possesses 31% identity with TLR4 of the oyster Pinctada imbricata, whose expression was significantly upregulated 6 h after LPS injection possesses both death and TIR domains and displays 97% sequence identity with Sepiella japonica MyD88, another cephalopod MyD88 significantly upregulated in the liver 24 h after LPS injection …”

Section: Resultsmentioning

confidence: 99%

“…So-TLRγ possesses 31% identity with TLR4 of the oyster Pinctada imbricata, whose expression was significantly upregulated 6 h after LPS injection. 123 So-MyD88 (myeloid differentiation factor 88), previously identified in ANG, 120 possesses both death and TIR domains and displays 97% sequence identity with Sepiella japonica MyD88, another cephalopod MyD88 significantly upregulated in the liver 24 h after LPS injection. 124 So-Tollip was identified in the WB proteome (Table 4); it possesses 68% sequence identity with Mizuhopecten yessoensis Tollip, which was upregulated after bacterial challenge.…”

Section: Involvement Of the Wb In Immunity And The Stress Responsementioning

confidence: 99%

-Omic Analysis of the Sepia officinalis White Body: New Insights into Multifunctionality and Haematopoiesis Regulation

Benoist

Corre

Bernay³

et al. 2020

J. Proteome Res.

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Cephalopods, like other Protostomes, lack an adaptive immune system and only rely on an innate immune system. The main immune cells are haemocytes, able to respond to pathogens and external attacks. First reports based on morphological observations suggested that the white body located in the optic sinuses of cuttlefish was at the origin of haemocytes. Combining transcriptomic and proteomic analyses, we identified several factors known to be involved in haematopoiesis in Vertebrate species in cuttlefish white body. Among these factors, members of the JAK-STAT signalling pathway were identified, some of them for the first time in a molluscan transcriptome and proteome. Immune factors such as members of the Toll/NF-κB signalling pathway, pattern recognition proteins and receptors, and members of the oxidative stress responses were also identified, and support an immune role of the white body. Both transcriptome and proteome analyses revealed that the white body harbours an intense metabolism concurrent with the haematopoietic function. Finally, a comparative analysis of the white body and haemocyte proteomes revealed many proteins in common, confirming previous morphological studies on the origin of haemocytes in cuttlefish. This molecular work demonstrates that the white body is multifunctional and provides bases for haematopoiesis regulation in cuttlefish.

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“…TLR1 and TLR2 were significantly up-regulated at 0 h, whereas TLR4 at 48 h, indicating that TLR4 may play a vital role in wound healing and immune response to the inserted nucleus and mantle graft. In a recent study on P. fucata , TLR4 was also found to increase significantly after implantation peaking at day 2 [65]. TLR4 is believed to initiate inflammation and tissue injury by responding to both bacterial endotoxin and multiple endogenous ligands, including heat-shock protein (HSP) [66].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles at different stages for formation of pearl sac and pearl in the pearl oyster Pinctada fucata

et al. 2019

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BackgroundThe most critical step in the pearl formation during aquaculture is issued to the proliferation and differentiation of outer epithelial cells of mantle graft into pearl sac. This pearl sac secretes various matrix proteins to produce pearls by a complex physiological process which has not been well-understood yet. Here, we aimed to unravel the genes involved in the development of pearl sac and pearl, and the sequential expression patterns of different shell matrix proteins secreted from the pearl sac during pearl formation by pearl oyster Pinctada fucata using high-throughput transcriptome profiling.ResultsPrincipal component analysis (PCA) showed clearly different gene expression profiles between earlier (before 1 week) and later stages (1 week to 3 months) of grafting. Immune-related genes were highly expressed between 0 h – 24 h (donor dependent) and 48 h – 1 w (host dependent), and in the course of wound healing process pearl sac was developed by two weeks of graft transplantation. Moreover, for the first time, we identified some stem cell marker genes including ABCG2, SOX2, MEF2A, HES1, MET, NRP1, ESR1, STAT6, PAX2, FZD1 and PROM1 that were expressed differentially during the formation of pearl sac. The expression profiling of 192 biomineralization-related genes demonstrated that most of the shell matrix proteins (SMPs) involved in prismatic layer formation were first up-regulated and then gradually down-regulated indicating their involvement in the development of pearl sac and the onset of pearl mineralization. Most of the nacreous layer forming SMPs were up-regulated at 2 weeks after the maturation of pearl sac. Nacrein, MSI7 and shematrin involved in both layer formation were highly expressed during 0 h – 24 h, down-regulated up to 1 week and then up-regulated again after accomplishment of pearl sac formation.ConclusionsUsing an RNA-seq approach we unraveled the expression pattern of the key genes involved in the development of pearl sac and pearl as a result of host immune response after grafting. These findings provide valuable information in understanding the molecular mechanism of pearl formation and immune response in P. fucata.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5579-3) contains supplementary material, which is available to authorized users.

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A novel toll-like receptor from the pearl oyster Pinctada fucata martensii is induced in response to stress

Cited by 20 publications

References 51 publications

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

Immune Transcripts of the Pacific Oyster, Crassostrea gigas Larvae that Change in Expression as a Result of Bacteria Challenge

-Omic Analysis of the Sepia officinalis White Body: New Insights into Multifunctionality and Haematopoiesis Regulation

Gene expression profiles at different stages for formation of pearl sac and pearl in the pearl oyster Pinctada fucata

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