A novel transcription factor JcNAC1 response to stress in new model woody plant Jatropha curcas

Qin, Xiaobo; Zheng, Xinglong; Huang, Xiaoqi; Lii, Yifan; Shao, Chang‐Lun; Xu, Ying; Chen, Fang

doi:10.1007/s00425-013-1993-y

Cited by 15 publications

(9 citation statements)

References 48 publications

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“…oryzae infection in rice [35]. Overexpression of JcNAC1 in Jatropha curcas can improve the sensitivity of plants to pathogen infection and change the expression of some defense-related genes [36]. Over expression of ATAF2 in Arabidopsis can enhance the sensitivity of soil borne disease infection Fusarium oxysporum and inhibit the expression of some defense related genes [37].…”

Section: Discussionmentioning

confidence: 99%

A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

Zhang

Ali

Feng

et al. 2018

IJMS

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SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response.

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Section: Discussionmentioning

confidence: 99%

A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

Zhang

Ali

Feng

et al. 2018

IJMS

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“…Due to several advantages of Agrobacterium -mediated genetic transformation, including the defined integration of transgenes, potentially low copy number, and preferential integration into transcriptionally active regions of the chromosome (Newell 2000 ), this method is the most widely used to generate transgenic J. curcas . Several A. tumefaciens strains have been used in the genetic transformation of J. curcas , including LBA4404 (Kumar et al 2010 ; Li et al 2008 ; Misra et al 2012 ), EHA105 (He et al 2009 ; Jaganath et al 2014 ; Mazumdar et al 2010 ), GV3101 (Qin et al 2014 ), and AGL1 (Mao et al 2009 ). The types of selectable markers and the selection pressure also play important roles in the genetic transformation of different explants.…”

Section: Introductionmentioning

confidence: 99%

An efficient protocol for Agrobacterium-mediated transformation of the biofuel plant Jatropha curcas by optimizing kanamycin concentration and duration of delayed selection

Tang

et al. 2015

Plant Biotechnol Rep

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Jatropha curcas is considered a potential biodiesel feedstock crop. Currently, the value of J. curcas is limited because its seed yield is generally low. Transgenic modification is a promising approach to improve the seed yield of J. curcas. Although Agrobacterium-mediated genetic transformation of J. curcas has been pursued for several years, the transformation efficiency remains unsatisfying. Therefore, a highly efficient and simple Agrobacterium-mediated genetic transformation method for J. curcas should be developed. We examined and optimized several key factors that affect genetic transformation of J. curcas in this study. The results showed that the EHA105 strain was superior to the other three Agrobacterium tumefaciens strains for infecting J. curcas cotyledons, and the supplementation of 100 mM acetosyringone slightly increased the transient transformation frequency. Use of the appropriate inoculation method, optimal kanamycin concentration and appropriate duration of delayed selection also improved the efficiency of stable genetic transformation of J. curcas. The percentage of β-glucuronidase positive J. curcas shoots reached as high as 56.0 %, and 1.70 transformants per explant were obtained with this protocol. Furthermore, we optimized the root-inducing medium to achieve a rooting rate of 84.9 %. Stable integration of the T-DNA into the genomes of putative transgenic lines was confirmed by PCR and Southern blot analysis. Using this improved protocol, a large number of transgenic J. curcas plantlets can be routinely obtained within approximately 4 months. The detailed information provided here for each step of J. curcas transformation should enable successful implementation of this transgenic technology in other laboratories.Electronic supplementary materialThe online version of this article (doi:10.1007/s11816-015-0377-0) contains supplementary material, which is available to authorized users.

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“…In N. benthamiana, SPL6 plays a positive regulatory role in nucleotide binding rich leucine repeat (N TIR-NB-LRR) receptor-mediated plant natural immunity (Padmanabhan et al, 2013). Overexpression of JcNAC1 in Jatropha curcas can enhance susceptibility of plants to Botrytis cinerea infection and inhibit the expression of some defense-related marker genes (Qin et al, 2014). Overexpression of ATAF2 in Arabidopsis can enhance the sensitivity of plants to Fusarium oxysporum infection and repress the expression of pathogenesisrelated genes (Delessert et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Identification of Pepper CaSBP08 Gene in Defense Response Against Phytophthora capsici Infection

et al. 2020

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Little information is available on the role of Squamosa promoter binding protein (SBP)-box genes in pepper plants. This family of genes is known to have transcription characteristics specific to plants and to regulate plant growth, development, stress responses, and signal transduction. To investigate their specific effects in pepper (Capsicum annuum), we screened pepper SBP-box family genes (CaSBP genes) for Phytophthora capsici (P. capsici) resistance genes using virus-induced gene silencing. CaSBP08, CaSBP11, CaSBP12, and CaSBP13, which are associated with plant defense responses against P. capsici, were obtained from among fifteen identified CaSBP genes. The function of CaSBP08 was identified in pepper defense response against P. capsici infection in particular. CaSBP08 protein was localized to the nucleus. Silencing of CaSBP08 enhanced resistance to P. capsici infection. Following P. capsici inoculation, the malondialdehyde content, peroxidase activity, and disease index percentage of the CaSBP08-silenced plants decreased compared to the control. Additionally, the expression levels of other defense-related genes, especially those of CaBPR1 and CaSAR8.2, were more strongly induced in CaSBP08-silenced plants than in the control. However, CaSBP08 overexpression in Nicotiana benthamiana enhanced susceptibility to P. capsici infection. This work provides a foundation for the further research on the role of CaSBP genes in plant defense responses against P. capsici infection.

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A novel transcription factor JcNAC1 response to stress in new model woody plant Jatropha curcas

Cited by 15 publications

References 48 publications

A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

An efficient protocol for Agrobacterium-mediated transformation of the biofuel plant Jatropha curcas by optimizing kanamycin concentration and duration of delayed selection

Identification of Pepper CaSBP08 Gene in Defense Response Against Phytophthora capsici Infection

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