A novel trehalosamine isolated from Bacillus amyloliquefaciens and its antibacterial activities

Abstract

Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify active ingredient from Bacillus amyloliquefaciens GN59 and study its antibacterial activity. A novel compound was isolated from GN59 by column chromatography on silica gel and HPLC purification. The chemical structure was identified as α-dglucopyranosyl-(1 → 1′)-3′-amino-3′-deoxy-β-d-glucopyranoside (a,β-3-trehalosamine) on the basis of spectroscopic analysis. This is the first report about a,β-3-trehalosamine isolated from biological resources on an antibiotic activity against pathogenic bacterium. The 3′-neotrehalosamine displayed antibacterial activity across a broad spectrum of microorganisms, including different gram-positive and gram-negative bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.5 to 0.7 mg/mL. The results indicated that the 3′-neotrehalosamine from GN59 might be a potential candidate for bactericide.

Rediscovery of 4‐Trehalosamine as a Biologically Stable, Mass‐Producible, and Chemically Modifiable Trehalose Analog

“…To the best of our knowledge, other than 4-trehalosamine, the mono-amino trehalosamines 2-trehalosamine and 3-trehalosamine were reported as natural products obtained from actinomycetes, [81,82] and recently, α,β-3-trehalosamine was reported as a product of a bacillus. [83] Moreover, several studies have focused on the chemical synthesis of mono-amino trehalosamines, including 6-trehalosamine. [84][85][86][87] They could serve as good trehalose substitutes, similar to 4-trehalosamine in this study, although most of them are difficult to synthesize in sufficient amounts for practical applications.…”

Optimizing the Fermentation Conditions and Determining the Disease Control and Growth Promotion Function of Bacillus Amyloliquefaciens 3-5

“…As everyone knows, microorganisms are an almost unlimited source of natural products, many of which have potential therapeutic uses. In the context of the current study, the bacterium B. amyloliquefaciens has been widely used as a probiotic in the eld of biological control that its antibacterial compounds play a vital role in the prevention and control of plant, livestock and poultry diseases (Wang et al, 2020), and it has the advantages of safe, e ciency and green environmental protection.…”