A novel type of interplexiform amacrine cell in the mouse retina

Dedek, Karin; Breuninger, Tobias; Müller, Luis Pérez de Sevilla; Maxeiner, Stephan; Schultz, Konrad; Janssen‐Bienhold, Ulrike; Willecke, Klaus; Euler, Thomas; Weiler, Reto

doi:10.1111/j.1460-9568.2009.06808.x

Cited by 37 publications

(31 citation statements)

References 69 publications

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“…For example, in the wild-type mouse retina, ionotropic GABA receptors are expressed on HCs (Feigenspan and Weiler, 2004) acting as auto-receptors (Liu et al, 2013) but are not expressed on cones (Kemmler et al, 2014), whereas rd1 cones do express GABA A/C receptors (Pattnaik et al, 2000). Notably, also GABAergic interplexiform cells form synapses in the outer retina (Dedek et al, 2009) and therefore may participate in controlling the activity of neurons in the outer rd1 retina.…”

Section: Resultsmentioning

confidence: 99%

Synaptic remodeling generates synchronous oscillations in the degenerated outer mouse retina

Haq

Ueffing

Zrenner

et al. 2014

Front. Neural Circuits

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During neuronal degenerative diseases, neuronal microcircuits undergo severe structural alterations, leading to remodeling of synaptic connectivity. The functional consequences of such remodeling are mostly unknown. For instance, in mutant rd1 mouse retina, a common model for Retinitis Pigmentosa, rod bipolar cells (RBCs) establish contacts with remnant cone photoreceptors (cones) as a consequence of rod photoreceptor cell death and the resulting lack of presynaptic input. To assess the functional connectivity in the remodeled, light-insensitive outer rd1 retina, we recorded spontaneous population activity in retinal wholemounts using Ca2+ imaging and identified the participating cell types. Focusing on cones, RBCs and horizontal cells (HCs), we found that these cell types display spontaneous oscillatory activity and form synchronously active clusters. Overall activity was modulated by GABAergic inhibition from interneurons such as HCs and/or possibly interplexiform cells. Many of the activity clusters comprised both cones and RBCs. Opposite to what is expected from the intact (wild-type) cone-ON bipolar cell pathway, cone and RBC activity was positively correlated and, at least partially, mediated by glutamate transporters expressed on RBCs. Deletion of gap junctional coupling between cones reduced the number of clusters, indicating that electrical cone coupling plays a crucial role for generating the observed synchronized oscillations. In conclusion, degeneration-induced synaptic remodeling of the rd1 retina results in a complex self-sustained outer retinal oscillatory network, that complements (and potentially modulates) the recently described inner retinal oscillatory network consisting of amacrine, bipolar and ganglion cells.

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Section: Resultsmentioning

confidence: 99%

Synaptic remodeling generates synchronous oscillations in the degenerated outer mouse retina

Haq

Ueffing

Zrenner

et al. 2014

Front. Neural Circuits

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“…In mammalian retina some of the GABAergic neurons in the inner nuclear layer are GABAergic interplexiform cells ( rodent : [32, 46–49]; cat : [17, 25, 32, 46, 50]; rabbit : [32, 51]; primate : [28, 52, 53]; human : [32, 54, 55]) (Figure 1). Although most of them contain a second neurotransmitter (dopamine, acetylcholine, NO) as well as GABA, types of interplexiform cell containing only GABA have also been described [49, 53].…”

Section: Gabaergic Neurons In Retinamentioning

confidence: 99%

Ionotropic GABA Receptors and Distal Retinal ON and OFF Responses

Popova

2014

Scientifica

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In the vertebrate retina, visual signals are segregated into parallel ON and OFF pathways, which provide information for light increments and decrements. The segregation is first evident at the level of the ON and OFF bipolar cells in distal retina. The activity of large populations of ON and OFF bipolar cells is reflected in the b- and d-waves of the diffuse electroretinogram (ERG). The role of gamma-aminobutyric acid (GABA), acting through ionotropic GABA receptors in shaping the ON and OFF responses in distal retina, is a matter of debate. This review summarized current knowledge about the types of the GABAergic neurons and ionotropic GABA receptors in the retina as well as the effects of GABA and specific GABAA and GABAC receptor antagonists on the activity of the ON and OFF bipolar cells in both nonmammalian and mammalian retina. Special emphasis is put on the effects on b- and d-waves of the ERG as a useful tool for assessment of the overall function of distal retinal ON and OFF channels. The role of GABAergic system in establishing the ON-OFF asymmetry concerning the time course and absolute and relative sensitivity of the ERG responses under different conditions of light adaptation in amphibian retina is also discussed.

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“…Immunohistochemistry was performed as described previously (Dedek et al, 2009). Briefly, the posterior eyecup was fixed for 15-30 min in 2-4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

“…Medium-field and wide-field amacrine (WFA) cells exhibit large dendritic arbors (up to 1 mm). These cells are GABAergic and comprise ϳ20 subtypes (Badea and Nathans, 2004;Lin and Masland, 2006;Pérez De Sevilla Müller et al, 2007;Dedek et al, 2009;Majumdar et al, 2009). WFA cells either mediate lateral interactions, which are often confined to a single stratum in the IPL (Hsueh et al, 2008), or provide local reciprocal inhibition, as was recently shown for A17 cells (Grimes et al, 2010).…”

Section: Introductionmentioning

confidence: 99%