A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation

Sun, Zhi‐Gang; Kong, Wei Hua; Zhang, Yan Jun; Shan, Yan Shen; Lu, Ji Ning; Gu, Zheng; Lin, Feng; Tso, J. Yun

doi:10.1038/sj.cr.7290125

Cited by 12 publications

(21 citation statements)

References 23 publications

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“…The data in Xenopus oocytes suggested that the activity of MAP kinase is essential for maintaining the activity of p90 rsk [41,42]. Previous work by us and others also showed that in mouse and rat oocytes, the change of MAP kinase activity is always followed by the change of p90 rsk activity during meiotic maturation, fertilization, and parthenogenetic activation [43][44][45].…”

Section: Discussionmentioning

confidence: 97%

Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtuble organization, asymmetric division and metaphase II arrest

et al. 2003

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In this study we used U0126, a potent and specific inhibitor of MEK, to study the roles of MEK/ERK/p90 rsk signaling pathway in the meiotic cell cycle of mouse oocytes. The phosphorylation of MAP kinase and p90 rsk in the oocytes treated with 1.5 M U0126 was the same as that in oocytes cultured in drug-free medium. With 1.5 M U0126 treatment, the spindles appeared normal as they formed in oocytes, but failed to maintain its structure. Instead, the spindle lost one pole or elongated extraordinarily. After further culture, some oocytes extruded gigantic polar bodies (>30 m) that later divided into two small ones. Some oocytes underwent symmetric division and produced two equal-size daughter cells in which normal spindles formed. In oocytes with different division patterns, MAP kinase was normally phosphorylated. When the concentration of U0126 was increased to 15 M, the phosphorylation of both MAPK and p90 rsk were inhibited, while symmetric division was decreased. When incubating in medium containing 15 M U0126 for 14 h, oocytes were activated, but part of them failed to emit polar bodies. MII oocytes were also activated by 15 M U0126, at the same time the dephosphorylation of MAP kinase and p90 rsk was observed. Our results indicate that 1) MEK plays important but not indispensable roles in microtubule organization; 2) MEK keeps normal meiotic spindle morphology, targets peripheral spindle positioning and regulates asymmetric division by activating some unknown substrates other than MAP kinase /p90 rsk ; and 3) activation of MEK/ERK/p90 rsk cascade maintains MII arrest in mouse oocytes.

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Section: Discussionmentioning

confidence: 97%

Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtuble organization, asymmetric division and metaphase II arrest

et al. 2003

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“…We have found that UCH-L1 is involved in toad oocyte maturation, and toad UCH-L1 is considered to specifically bind to CDK1 in toad oocyte protein extracts (Sun et al 2002). So, in mouse oocytes UCH-L1 may be associated with the mitotic promoting factor (MPF), also known as maturation promoting factor, which consists of cyclin B1/B2 and CDK1 (or p34 cdc2 ).…”

Section: Discussionmentioning

confidence: 97%

“…1a). It has been reported that UCH-L1 binds to CDK1 in toad oocyte protein extracts (Sun et al 2002), so we examined the protein-protein interaction of UCH-L1 and CDK1, and found that UCH-L1 was able to bind to CDK1 in mouse ovarian protein extracts (Fig. 1a).…”

Section: Ptov1 Is Specifically Bound To Uch-l1 In Ovarian Protein Extmentioning

confidence: 93%

“…The protein concentration of each total protein extract was determined by the Bradford assay. Western blot (WB) analysis was carried out as previously described (Sun et al 2002). The proteins were detected with the following antibodies: rabbit anti-PTOV1 (1:500 dilution), rabbit anti-p34 cdc2 (1:1,000 dilution) and rabbit anti-UCH-L1 (1:200 dilution).…”

Section: Western Blot Analysismentioning

confidence: 99%

“…UCH-L1 exists an oocyte-specific distribution and participates in the regulation of oocyte maturation in toads (Sun et al 2002), and it contributes to the oocyte selective elimination in prepubertal mouse ovaries (Gu et al 2009). To elucidate the functions of UCH-L1 in oocyte development and maturation, we used mass spectrographic technology to search for putative interaction partners of UCH-L1 and found that PTOV1 was a putative interacting protein.…”

Section: Introductionmentioning

confidence: 99%

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PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development

Shi

Jia

et al. 2011

Histochem Cell Biol

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To investigate the biological significance of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) involvement in oocyte maturation, we screened for proteins that bound to UCH-L1 in mouse ovaries, and we found that the prostate tumor overexpressed-1 (PTOV1) protein was able to bind to UCH-L1. PTOV1 is highly expressed in prostate cancers and considered as a potential marker for carcinogenesis and the progress of prostate cancer. It was reported that PTOV1 plays an important role in cell cycle regulation, but its role in mammalian oocyte development and meiosis is still unclear. In this paper, it was found that the expression levels of PTOV1 in mouse ovaries progressively increased from prepubescence to adulthood. And we found by immunohistochemistry that PTOV1 spreaded in both the cytoplasm and nuclei of oocytes during prepuberty, but in normal adult mouse oocytes, it concentrated not only in nuclei but also on the plasma membrane, though in some oocytes with abnormal shapes, PTOV1 did not display the typical distribution patterns. In granulosa cells, however, it was found to locate in the cytoplasm at all the selected ages. In postnatal mouse ovaries (28 days), estradiol treatment induced the adult-specific distribution pattern of PTOV1 in oocytes. In addition, UCH-L1 was shown to be associated with CDK1, which participated in the regulation of cell cycle and oocyte maturation. Therefore, we propose that the distribution changes of PTOV1 are age-dependent, and significant for mouse oocyte development and maturation. Moreover, the discovery that PTOV1 is associated with UCH-L1 in mouse oocytes supports the explanations for that UCH-L1 is involved in oocyte development and maturation, especially under the regulation of estrogen.

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2‐D DIGE analysis of the budding yeast pH 6–11 proteome in meiosis

et al. 2010

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Meiosis is the cell division that generates haploid gametes from diploid precursors. To provide insight into the functional proteome of budding yeast during meiosis, a 2-D DIGE kinetic approach was used to study proteins in the pH 6-11 range. Nearly 600 protein spots were visualised and 79 spots exhibited statistically significant changes in abundance as cells progressed through meiosis. Expression changes of up to 41-fold were detected and protein sequence information was obtained for 48 spots. Single protein identifications were obtained for 21 spots including different gel mobility forms of 5 proteins. A large number of post-translational events are suggested for these proteins, including processing, modification and import. The data are incorporated into an online 2-DE map of meiotic proteins in budding yeast, which extends our initial DIGE investigation of proteins in the pH 4-7 range. Together, the analyses provide peptide sequence data for 84 protein spots, including 50 single-protein identifications and gel mobility isoforms of 8 proteins. The largest classes of identified proteins include carbon metabolism, protein catabolism, protein folding, protein synthesis and the oxidative stress response. A number of the corresponding genes are required for yeast meiosis and recent studies have identified similar classes of proteins expressed during mammalian meiosis. This proteomic investigation and the resulting protein reference map make an important contribution towards a more detailed molecular view of yeast meiosis.

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A novel ubiquitin carboxyl terminal hydrolase is involved in toad oocyte maturation

Cited by 12 publications

References 23 publications

Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtuble organization, asymmetric division and metaphase II arrest

Effects of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtuble organization, asymmetric division and metaphase II arrest

PTOV1 is associated with UCH-L1 and in response to estrogen stimuli during the mouse oocyte development

2‐D DIGE analysis of the budding yeast pH 6–11 proteome in meiosis

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