A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United Kingdom

Hornsey, Michael; Phee, Lynette; Wareham, David W.

doi:10.1128/aac.05108-11

Cited by 331 publications

(271 citation statements)

References 12 publications

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“…Mussi et al (2005) have proved that ISAba125 causes insertional inactivation of the CarO OMP in A. baumannii strains, leading to a carbapenem-resistant profile. The insertion element ISAba125, first identified in A. baumannii, is widely distributed and has also been associated with gene variants of the bla NDM carbapenemase in the family Enterobacteriaceae (Hornsey et al, 2011;Nordmann et al, 2012;Pfeifer et al, 2011). The results obtained in this study show that IS elements such as ISAba125 could modulate antibiotic resistance depending on the antibiotic treatment of the patient as the isolates used in this study were of clinical origin.…”

Section: Discussionmentioning

confidence: 53%

Role of ISAba1 and ISAba125 in governing the expression of bla ADC in clinically relevant Acinetobacter baumannii strains resistant to cephalosporins

Lopes

Amyes

2012

Journal of Medical Microbiology

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Acinetobacter baumannii is a multi-resistant opportunistic nosocomial pathogen responsible for several outbreaks worldwide. It can cause several infections at various sites of the body. One of the main infections caused by this bacterium is ventilator-associated pneumonia in patients in intensive care units. Treating these infections is becoming difficult because of the high resistance to antimicrobial agents. This study compared the expression of the chromosomally encoded bla ADC gene in isolates having ISAba1, ISAba125 and no insertion upstream of the bla ADC gene in A. baumannii clinical isolates. It showed that the expression of bla ADC was six times greater when ISAba125 was present upstream of the gene in comparison with the constitutively expressed bla ADC gene with no insertion present upstream. The study indicated that ISAba125 has better promoters than ISAba1 and this is responsible for the overexpression of the bla ADC gene as they share considerable homology to the well-established Escherichia coli promoters. The "10 box of ISAba125 formed a fusion promoter with the "35 box of the bla ADC gene causing the bla ADC gene to be significantly overexpressed. The ability to upregulate the expression of bla ADC with the assistance of different insertion elements such as ISAba1 and ISAba125 has become an important factor in A. baumannii resistance to cephalosporins. INTRODUCTIONThe predominant mechanism of b-lactam resistance in Gram-negative bacteria is the synthesis of b-lactamases (Philippon et al., 2002). Following the introduction of highly stable cephalosporins, the carbapenems and monobactams, resistance initially appeared in organisms such as Enterobacter cloacae, Citrobacter freundii, Serratia marcescens and Pseudomonas aeruginosa, where mutations in the chromosome lead to the overproduction of the AmpC blactamase. This confers resistance to oxyimino-and 7-alphamethoxy-cephalosporins and monobactams (Sanders, 1987). Class C b-lactamases produced by Gram-negative bacteria can hydrolyse many b-lactam antibiotics including the cephamycins and oxyimino-cephalosporins (Philippon et al., 2002;Sanders, 1987;Caroff et al., 2000). These enzymes can also hydrolyse monobactams, such as aztreonam, to a lesser extent (Bush et al., 1995;Mammeri & Nordmann, 2007). Acinetobacter baumannii is a Gramnegative, non-fermenting coccobacillus, which is associated with serious nosocomial infections (Cisneros & Rodríguez-Baño, 2002;Levin et al., 2003). Antimicrobial treatment of such infections is difficult due to their multidrug resistance profile (Corvec et al., 2003). The AmpC enzyme from multiresistant A. baumannii was first sequenced from a Spanish isolate (Bou & Martínez-Beltrán, 2000). This constitutively expressed AmpC enzyme shares low similarity with Enterobacteriaceae AmpC cephalosporinases (Corvec et al., 2003). High-level expression of AmpC b-lactamase is a mechanism of chromosomally mediated resistance to cephalosporins in A. baumannii (Bou & Martínez-Beltrán, 2000). Transposable elements are important motors...

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Section: Discussionmentioning

confidence: 53%

Role of ISAba1 and ISAba125 in governing the expression of bla ADC in clinically relevant Acinetobacter baumannii strains resistant to cephalosporins

Lopes

Amyes

2012

Journal of Medical Microbiology

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“…The E. coli strains that were isolated from the media containing carbapenem were further analyzed to determine the mechanisms involved in resistance to carbapenems using a multiplex PCR (Dallenne, Costa, Decré, Favier, & Arlet, 2010; Hornsey, Phee, & Wareham, 2011). Briefly, the presence of the most prevalent carbapenemase genes (including bla KPC , bla VIM , bla OXA‐48 group, and bla IMP ) was assessed by multiplex PCR, as previously described (Dallenne et al., 2010) and bla NDM gene presence was investigated by PCR assay (Hornsey et al., 2011).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the presence of the most prevalent carbapenemase genes (including bla KPC , bla VIM , bla OXA‐48 group, and bla IMP ) was assessed by multiplex PCR, as previously described (Dallenne et al., 2010) and bla NDM gene presence was investigated by PCR assay (Hornsey et al., 2011). Metallo‐β‐lactamase production was assessed by the Carbapenemase/Metallo‐β‐Lactamase Confirmation Identification Pack (Rosco Diagnostic Neo‐Sensitabs ™ , Eurobio, Courtaboeuf, France).…”

Section: Methodsmentioning

confidence: 99%

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Vittecoq

Laurens

Brazier

et al. 2017

Ecology and Evolution

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Acquired carbapenemases currently pose one of the most worrying public health threats related to antimicrobial resistance. A NDM‐1‐producing Salmonella Corvallis was reported in 2013 in a wild raptor. Further research was needed to understand the role of wild birds in the transmission of bacteria resistant to carbapenems. Our aim was to investigate the presence of carbapenem‐resistant Escherichia coli in gulls from southern France. In 2012, we collected 158 cloacal swabs samples from two gull species: yellow‐legged gulls (Larus michahellis) that live in close contact with humans and slender‐billed gulls (Chroicocephalus genei) that feed at sea. We molecularly compared the carbapenem‐resistant bacteria we isolated through culture on selective media with the carbapenem‐susceptible strains sampled from both gull species and from stool samples of humans hospitalized in the study area. The genes coding for carbapenemases were tested by multiplex PCR. We isolated 22 carbapenem‐resistant E. coli strains from yellow‐legged gulls while none were isolated from slender‐billed gulls. All carbapenem‐resistant isolates were positive for bla VIM ‐1 gene. VIM‐1‐producing E. coli were closely related to carbapenem‐susceptible strains isolated from the two gull species but also to human strains. Our results are alarming enough to make it urgently necessary to determine the contamination source of the bacteria we identified. More generally, our work highlights the need to develop more bridges between studies focusing on wildlife and humans in order to improve our knowledge of resistant bacteria transmission routes.

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“…ST405 with various types of CTX-Ms has a worldwide distribution (7,14,19,35), while ST648 with CTX-M-15 and -32 is present in poultry from Spain (8) and humans in China (44) and the Netherlands (41). ST405 and ST648 are also associated with NDM ␤-lactamases (11,21).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology over an 11-Year Period (2000 to 2010) of Extended-Spectrum β-Lactamase-Producing Escherichia coli Causing Bacteremia in a Centralized Canadian Region

et al. 2012

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A study was designed to assess the importance of sequence types among extended-spectrum ␤-lactamase (ESBL)-producing Escherichia coli isolates causing bacteremia over an 11-year period (2000 to 2010) in a centralized Canadian region. A total of 197 patients with incident infections were identified; the majority presented with community-onset urosepsis, with a significant increase in the prevalence of ESBL-producing E. coli during the later part of the study. The majority of E. coli isolates produced either CTX-M-15 or CTX-M-14. We identified 7 different major sequence types among 91% of isolates (i.e., the ST10 clonal complex, ST38, ST131, ST315, ST393, ST405, and ST648) and provided insight into their clinical and molecular characteristics. ST38 was the most antimicrobial-susceptible sequence type and predominated during 2000 to 2004 but disappeared after 2008. ST131 was the most antimicrobial-resistant sequence type, and the influx of a single pulsotype of this sequence type was responsible for the significant increase of ESBL-producing E. coli strains since 2007. During 2010, 49/63 (78%) of the ESBL-producing E. coli isolates belonged to ST131, and this sequence type had established itself as a major drug-resistant pathogen in Calgary, Alberta, Canada, posing an important new public health threat within our region. We urgently need well-designed epidemiological and molecular studies to understand the dynamics of transmission, risk factors, and reservoirs for E. coli ST131. This will provide insight into the emergence and spread of this multiresistant sequence type.

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A Novel Variant, NDM-5, of the New Delhi Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli ST648 Isolate Recovered from a Patient in the United Kingdom

Cited by 331 publications

References 12 publications

Role of ISAba1 and ISAba125 in governing the expression of bla ADC in clinically relevant Acinetobacter baumannii strains resistant to cephalosporins

Role of ISAba1 and ISAba125 in governing the expression of bla ADC in clinically relevant Acinetobacter baumannii strains resistant to cephalosporins

VIM‐1 carbapenemase‐producing Escherichia coli in gulls from southern France

Molecular Epidemiology over an 11-Year Period (2000 to 2010) of Extended-Spectrum β-Lactamase-Producing Escherichia coli Causing Bacteremia in a Centralized Canadian Region

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