A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression in the freshwater crayfish, Procambarus clarkii

Wang, Zheng; Chen, Yihong; Dai, Yunjia; Tan, Jing-Min; Huang, Ying; Lan, Jiang-Feng; Ren, Qian

doi:10.1016/j.fsi.2014.12.038

Cited by 81 publications

(30 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Drosophila, the Toll signal pathway is activated by fungi and Gram-positive bacteria to regulate expression of AMPs to defend against pathogen invasion (40,41). In shrimp, the Toll pathway plays an important role in regulating AMP expression (42,43). In this report, we find that the bacterial clearance and survival rate of Mj-Dorsal-silenced shrimp infected with S. aureus had declined significantly, suggesting that the Toll pathway plays a central role in host defense against S. aureus in shrimp.…”

Section: Discussionmentioning

confidence: 99%

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

Sun

Lan

Shi

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

The Toll signaling pathway plays an important role in the innate immunity of Drosophila melanogaster and mammals. The activation and termination of Toll signaling are finely regulated in these animals. Although the primary components of the Toll pathway were identified in shrimp, the functions and regulation of the pathway are seldom studied. We first demonstrated that the Toll signaling pathway plays a central role in host defense against Staphylococcus aureus by regulating expression of antimicrobial peptides in shrimp. We then found that ␤-arrestins negatively regulate Toll signaling in two different ways. ␤-Arrestins interact with the C-terminal PEST domain of Cactus through the arrestin-N domain, and Cactus interacts with the RHD domain of Dorsal via the ankyrin repeats domain, forming a heterotrimeric complex of ␤-arrestin⅐Cactus⅐ Dorsal, with Cactus as the bridge. This complex prevents Cactus phosphorylation and degradation, as well as Dorsal translocation into the nucleus, thus inhibiting activation of the Toll signaling pathway. ␤-Arrestins also interact with non-phosphorylated ERK (extracellular signal-regulated protein kinase) through the arrestin-C domain to inhibit ERK phosphorylation, which affects Dorsal translocation into the nucleus and phosphorylation of Dorsal at Ser 276 that impairs Dorsal transcriptional activity. Our study suggests that ␤-arrestins negatively regulate the Toll signaling pathway by preventing Dorsal translocation and inhibiting Dorsal phosphorylation and transcriptional activity.

show abstract

Section: Discussionmentioning

confidence: 99%

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

Sun

Lan

Shi

et al. 2016

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tolls identified in Litopenaeus vannamei ( 28 , 29 ), Fenneropenaeus chinensis ( 30 ), Penaeus monodon ( 31 , 32 ), Marsupenaeus japonicus ( 33 ), and Procambarus clarkii ( 34 , 35 ) play important roles in shrimp innate immunity. LvToll1–3 responded to Vibrio alginolyticus and WSSV infections in L. vannamei ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

“…FcToll and MjToll respond to various immune challenges ( 30 , 33 ). PcToll and PcToll2 regulate AMP expression after challenged with Vibrio anguillarum in P. clarkii ( 34 , 35 ). LvToll in L. vannamei regulates AMP expression after challenge with V. anguillarum and Micrococcus lysodeikticus ( 28 ).…”

Section: Introductionmentioning

confidence: 99%

Activation of Toll Pathway Is Different between Kuruma Shrimp and Drosophila

Sun

et al. 2017

Front. Immunol.

View full text Add to dashboard Cite

The Toll pathway is essential for inducing an immune response to defend against bacterial invasion in vertebrates and invertebrates. Although Toll receptors and the transcription factor Dorsal were identified in different shrimp, relatively little is known about how the Toll pathway is activated or the function of the pathway in shrimp antibacterial immunity. In this study, three Tolls (Toll1–3) and the Dorsal were identified in Marsupenaeus japonicus. The Toll pathway can be activated by Gram-positive (G+) and Gram-negative (G−) bacterial infection. Unlike Toll binding to Spätzle in Drosophila, shrimp Tolls could directly bind to pathogen-associated molecular patterns from G+ and G− bacteria, resulting in Dorsal translocation into nucleus to regulate the expression of different antibacterial peptides (AMPs) in the clearance of infected bacteria. These findings suggest that shrimp Tolls are pattern recognition receptors and the Toll pathway in shrimp is different from the Drosophila Toll pathway but identical with the mammalian Toll-like receptor pathway in its activation and antibacterial functions.

show abstract

“…These results suggested that crab AMP genes could be modulated by the host Toll pathway, which is also the case in Drosophila (Lemaitre and Hoffmann, 2007). Similarly, in Procambarus clarkii, PcToll was involved in the regulation of Crus expression (Wang et al, 2015). The knockdown of Dorsal in M. rosenbergii resulted in the suppression of Crus transcripts (Huang X. et al, 2016).…”

Section: Discussionmentioning

confidence: 98%

Host MicroRNA-217 Promotes White Spot Syndrome Virus Infection by Targeting Tube in the Chinese Mitten Crab (Eriocheir sinensis)

Huang

Han

Wang

et al. 2017

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs), a group of small molecule non-encoding RNAs, are key post-transcriptional regulators of gene expression that are implicated in many biological processes. In the current study, miR-217 from Eriocheir sinensis was selected for studying its roles during host–virus interaction. Overexpression or silencing of miR-217 led to considerable effects on white spot syndrome virus (WSSV) replication, implying that miR-217 played a positive role in WSSV infection. In insect High Five cells, miR-217 significantly inhibited Tube gene expression by binding to the 3′-untranslated region of the Tube. Overexpression of miR-217 in crab led to downregulation of tube expression. Knockdown of Tube in vivo led to significant enhancement of WSSV infection and inhibited the expression of five antimicrobial peptide (AMP) genes (Anti-lipopolysaccharide factor ALF1, ALF2, ALF3; Crustin Crus1, Crus2) in WSSV-challenged crabs. Overexpression of miR-217 also led to downregulation of these AMP genes in WSSV-challenged crabs. Our results showed that host miRNA played positive roles in virus infection by regulation of host tube gene, which is the key component of Toll signaling pathway.

show abstract

A novel vertebrates Toll-like receptor counterpart regulating the anti-microbial peptides expression in the freshwater crayfish, Procambarus clarkii

Cited by 81 publications

References 39 publications

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

β-Arrestins Negatively Regulate the Toll Pathway in Shrimp by Preventing Dorsal Translocation and Inhibiting Dorsal Transcriptional Activity

Activation of Toll Pathway Is Different between Kuruma Shrimp and Drosophila

Host MicroRNA-217 Promotes White Spot Syndrome Virus Infection by Targeting Tube in the Chinese Mitten Crab (Eriocheir sinensis)

Contact Info

Product

Resources

About