A novel wheat α‐amylase inhibitor gene, <i><scp>TaHPS</scp></i>, significantly improves the salt and drought tolerance of transgenic <i>Arabidopsis</i>

Xiao, Yanhong; Huang, Xi; Shen, Yinzhu; Huang, Zhenyu

doi:10.1111/j.1399-3054.2012.01707.x

Cited by 15 publications

(10 citation statements)

References 37 publications

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“…First, high levels of osmotic stress (> 100 mM NaCl, > 200 mM mannitol and > 20% Downloaded from https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/eraa037/5714152 by Ghent University user on 27 January 2020 A c c e p t e d M a n u s c r i p t 17 PEG6000) induce short-term transcriptome responses that are comparable between different compounds (comparison between the dataset of Kreps et al (2002) and Matsui et al (2008)) and that correlate with severe dehydration responses (CCSpearman = 0.74) (Matsui et al, 2008). As a consequence, many mutants with an increased tolerance to severe osmotic stress also survive better when grown in soil and exposed to severe dehydration stress (Gamboa et al, 2013;Lü et al, 2013;Song et al, 2013;Xiao et al, 2013;Cho et al, 2014;Kim et al, 2014;Liu et al, 2014;Qin et al, 2014;Cai et al, 2015;Zhao et al, 2015;Liu et al, 2019). These mutants often carry mutations in genes involved in early, general stress response pathways such as ROS-and Ca 2+ -signaling (Fig.…”

Section: Added Value Of Osmotic Stress Setups For Future Drought Strementioning

confidence: 99%

Plant growth under suboptimal water conditions: early responses and methods to study them

Dubois

Inzé

2020

Journal of Experimental Botany

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Drought stress forms a major environmental constraint during the life cycle of plants, often decreasing plant yield and in extreme cases threatening survival. The molecular and physiological responses induced by drought have been the topic of extensive research during the past decades. Because soil-based approaches to studying drought responses are often challenging due to low throughput and insufficient control of the conditions, osmotic stress assays in plates were developed to mimic drought. Addition of compounds such as polyethylene glycol, mannitol, sorbitol, or NaCl to controlled growth media has become increasingly popular since it offers the advantage of accurate control of stress level and onset. These osmotic stress assays enabled the discovery of very early stress responses, occurring within seconds or minutes following osmotic stress exposure. In this review, we construct a detailed timeline of early responses to osmotic stress, with a focus on how they initiate plant growth arrest. We further discuss the specific responses triggered by different types and severities of osmotic stress. Finally, we compare short-term plant responses under osmotic stress versus in-soil drought and discuss the advantages, disadvantages, and future of these plate-based proxies for drought.

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Section: Added Value Of Osmotic Stress Setups For Future Drought Strementioning

confidence: 99%

Plant growth under suboptimal water conditions: early responses and methods to study them

Dubois

Inzé

2020

Journal of Experimental Botany

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“…Alpha-amylase isozymes in barley are classified into type A (low pI) and type B (high pI), with at least two isozymes in each type [46]. The alpha-amylase genes in wheat consist of three subfamilies [47] and analyses of alpha-amylase in germinating wheat seeds revealed 27 isozymes [48].…”

Section: Classification Of Alpha Amylasementioning

confidence: 99%

The Rice Alpha-Amylase, Conserved Regulator of Seed Maturation and Germination

Damaris

Lin

Yang

et al. 2019

IJMS

151

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Alpha-amylase, the major form of amylase with secondary carbohydrate binding sites, is a crucial enzyme throughout the growth period and life cycle of angiosperm. In rice, alpha-amylase isozymes are critical for the formation of the storage starch granule during seed maturation and motivate the stored starch to nourish the developing seedling during seed germination which will directly affect the plant growth and field yield. Alpha-amylase has not yet been studied intensely to understand its classification, structure, expression trait, and expression regulation in rice and other crops. Among the 10-rice alpha-amylases, most were exclusively expressed in the developing seed embryo and induced in the seed germination process. During rice seed germination, the expression of alpha-amylase genes is known to be regulated negatively by sugar in embryos, however positively by gibberellin (GA) in endosperm through competitively binding to the specific promoter domain; besides, it is also controlled by a series of other abiotic or biotic factors, such as salinity. In this review, we overviewed the research progress of alpha-amylase with focus on seed germination and reflected on how in-depth work might elucidate its regulation and facilitate crop breeding as an efficient biomarker.

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“…Ionic stress, osmotic stress, nutritional imbalance, and oxidative damage are the main causes for salt injury of plants, among which ionic stress is the primary factor ( Xiao et al, 2013 ; Adem et al, 2014 ). Sodium chloride is the most common salt in environment.…”

Section: Introductionmentioning

confidence: 99%

GmCLC1 Confers Enhanced Salt Tolerance through Regulating Chloride Accumulation in Soybean

et al. 2016

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The family of chloride channel proteins that mediate Cl- transportation play vital roles in plant nutrient supply, cellular action potential and turgor pressure adjustment, stomatal movement, hormone signal recognition and transduction, Cl- homeostasis, and abiotic and biotic stress tolerance. The anionic toxicity, mainly caused by chloride ions (Cl-), on plants under salt stress remains poorly understood. In this work, we investigated the function of soybean Cl-/H+ antiporter GmCLC1 under salt stress in transgenic Arabidopsis thaliana, soybean, and yeast. We found that GmCLC1 enhanced salt tolerance in transgenic A. thaliana by reducing the Cl- accumulation in shoots and hence released the negative impact of salt stress on plant growth. Overexpression of GmCLC1 in the hairy roots of soybean sequestered more Cl- in their roots and transferred less Cl- to their shoots, leading to lower relative electrolyte leakage values in the roots and leaves. When either the soybean GmCLC1 or the yeast chloride transporter gene, GEF1, was transformed into the yeast gef1 mutant, and then treated with different chloride salts (MnCl2, KCl, NaCl), enhanced survival rate was observed. The result indicates that GmCLC1 and GEF1 exerted similar effects on alleviating the stress of diverse chloride salts on the yeast gef1 mutant. Together, this work suggests a protective function of GmCLC1 under Cl- stress.

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A novel wheat α‐amylase inhibitor gene, TaHPS, significantly improves the salt and drought tolerance of transgenic Arabidopsis

Cited by 15 publications

References 37 publications

Plant growth under suboptimal water conditions: early responses and methods to study them

Plant growth under suboptimal water conditions: early responses and methods to study them

The Rice Alpha-Amylase, Conserved Regulator of Seed Maturation and Germination

GmCLC1 Confers Enhanced Salt Tolerance through Regulating Chloride Accumulation in Soybean

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