A novel whole-cell biosensor for the determination of trichloroethylene

Chee, Gab-Joo

doi:10.1016/j.snb.2016.07.034

Cited by 23 publications

(15 citation statements)

References 29 publications

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“…The aerobic biodegradation of TCE was previously reported . Specifically, TCE can be biodegraded through cometabolic pathways by either aerobic or anaerobic bacteria, in the presence of cosubstrates . A study reports that the aerobic bacterium Pseudomonas sp.…”

Section: Resultsmentioning

confidence: 98%

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Zein-Bonded Graphene and Biosurfactants Enable the Electrokinetic Clean-Up of Hydrocarbons

et al. 2021

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Nonaqueous phase liquids (NAPL, e.g., hydrocarbons and chlorinated compounds) are common groundwater pollutants. Electrokinetic remediation of NAPLs uses electric fields to draw them toward electrodes and remove them from groundwater. The treatment requires NAPL mobility. Emulsification increases mobility, but at a risk for downstream receptors. We propose using alkaline aqueous solutions of zein and graphene nanoparticles (GNP) to form conductive materials, which could also act as barriers to control NAPL migration. Alkaline zein−GNP solutions can be injected in the polluted soil and solidified by neutralizing the pH (e.g., with glacial acetic acid, GAA). Shear rheology experiments showed that zein−GNP composites were cohesive, and voltammetry showed that GNP increased electrical conductivity of zein-based materials by 3.5 times. Gas chromatography−mass spectroscopy (GC−MS) demonstrated that the electrokinetic treatment of model sandy aquifers yielded >60% and ∼47% removal of emulsified toluene in freshwater and in salt solutions, respectively (with 30 min treatment using a 10 V differential voltage between a zein−GNP and an aluminum electrode. NaCl was used as model salt contaminant. The conductivity of surfactant solutions was lower in saline water than in freshwater, explaining differences in toluene removal. Toluene−water emulsions were stabilized using the natural surfactants lecithin and saponin. These surfactants acted synergistically in stabilizing emulsions in either freshwater or salt solutions. Lecithin and saponin likely interacted at toluene−water interfaces, as indicated by the morphology, interfacial tension and compressional rigidity of toluene−water interfaces with both components (relative to interfaces of either lecithin or saponin alone). The compressional behavior of interfacial films was well-described by the Marczak model. Electrokinetic treatment of saturated model sandy aquifers also decreased the turbidity of emulsions of water and either tricholoroethylene (TCE, by ∼41%) or diesel (by ∼75%), in the presence of a bacterial biosurfactant. This decrease was used as semiquantitative indicator of NAPL removal from water.

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Section: Resultsmentioning

confidence: 98%

“…A study reports that the aerobic bacterium Pseudomonas sp. strain ASA86 degraded TCE, activating toluene dioxygenase in the presence of toluene . Here, we exclusively focused on the effectiveness of biosurfactants produced by P. aeruginosa and we did not explore the ability of P. aeruginosa to biodegrade TCE.…”

Section: Resultsmentioning

confidence: 99%

Zein-Bonded Graphene and Biosurfactants Enable the Electrokinetic Clean-Up of Hydrocarbons

et al. 2021

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Section: Chemical Sensorsmentioning

confidence: 99%

“…A novel microbial biosensor based on Pseudomonas sp. strain ASA86 was designed by Gab-Joo Chee, utilizing a porous cellulose nitrate membrane with a chloride ion electrode to detect TCE (Chee 2016 ). After degradation of TCE by bacterium in the biosensor, chloride ions would be released, which can be detected by the ion electrode and output signal then converted into TCE value (Fig.…”

Section: Chemical Sensorsmentioning

confidence: 99%

Recent advances in cellulose-based membranes for their sensing applications

Fan

Zhang

Li³

et al. 2020

Cellulose

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In recent years, sensing applications have played a very important role in various fields. As a novel natural material, cellulose-based membranes with many merits can be served as all kinds of sensors. This review summarizes the recent progress of cellulose membranes as sensors, mainly focusing on their preparation processes and sensing properties. In addition, the opportunities and challenges of cellulose membrane-based sensors are also prospected. This review provides some references for the design of cellulose membrane materials for sensing applications in the future.

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“…A microbial sensor is a most commonly used tool in the water environment monitoring field. Based on the multiple intracellular enzyme systems in a microbial cell, the biosensor provides inherent benefits in multitasking for catalyzing the complex samples in the environment (e.g., for degrading the mixture composed of multiple organic compounds). − However, for fabricating a biosensor, the microbes are usually needed to be immobilized, which will expose the cells to an unnatural environment. Thus, the activity of immobilized cells will be changed − and the biosensor’s performance will be affected.…”

mentioning

confidence: 99%

How to Identify the “LIVE/DEAD” States of Microbes Related to Biosensing

Liu

Zhang

et al. 2019

ACS Sens.

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In this work, we fabricated a microbial biosensor with long-term stability, which relied on microbial activity. Activity of the microbe was commonly estimated by LIVE/DEAD assay and the propidium iodide (PI)-stained one was judged as dead. Herein, we proposed the utilization of a physiological state of microbes, which was neither live nor dead but between them. In this state, microbes represented a high PI-stained ratio but still had catalytic ability. This microbial state was obtained by forming the biofilm under the conditions of poor nutrition and low temperature. Thus, the dividing and proliferating ability of the microbes in the biofilm was weak, which was beneficial for long-term stability. This mechanism was further confirmed by the biosensors made from multifarious substrate materials, including graphene-based gel, biomass-based gel, graphite felt, and poly(vinyl chloride). This biosensor was applied to water pollution monitoring in the laboratory for 2 years and then was integrated into a multiparameter water quality monitoring station on a local lake for 2.5 years.

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A novel whole-cell biosensor for the determination of trichloroethylene

Cited by 23 publications

References 29 publications

Zein-Bonded Graphene and Biosurfactants Enable the Electrokinetic Clean-Up of Hydrocarbons

Zein-Bonded Graphene and Biosurfactants Enable the Electrokinetic Clean-Up of Hydrocarbons

Recent advances in cellulose-based membranes for their sensing applications

How to Identify the “LIVE/DEAD” States of Microbes Related to Biosensing

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