A Novel Y-Specific Long Non-Coding RNA Associated with Cellular Lipid Accumulation in HepG2 cells and Atherosclerosis-related Genes

Molina, Elsa; Chew, Guat Siew; Myers, Stephen; Clarence, Elyse M; Eales, James; Tomaszewski, Maciej; Charchar, Fadi J.

doi:10.1038/s41598-017-17165-9

Cited by 31 publications

(19 citation statements)

References 44 publications

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“…Sawaya et al observed, using quantitative PCR, that all systemic sclerosis (SSc) samples were positive for male DNA compared with higher levels of microchimerism in clinically unaffected SSc skin [22]. In fact, several groups have indicated the possibility that foetal microchimerism may actually render a benefit to the mother’s health in certain conditions [32, 39, 41–46].…”

Section: Discussionmentioning

confidence: 99%

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Bhat

Sharma

Roy

et al. 2019

Reprod Biol Endocrinol

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Background Previous studies, which were primarily based on the fluorescent in-situ hybridisation (FISH) technique, revealed conflicting evidence regarding male foetal microchimerism in endometriosis. FISH is a relatively less sensitive technique, as it is performed on a small portion of the sample. Additionally, the probes used in the previous studies specifically detected centromeric and telomeric regions of Y chromosome, which are gene-sparse heterochromatised regions. In the present study, a panel of molecular biology tools such as qPCR, expression microarray, RNA-seq and qRT-PCR were employed to examine the Y chromosome microchimerism in the endometrium using secretory phase samples from fertile and infertile patients with severe (stage IV) ovarian endometriosis (OE) and without endometriosis. Methods Microarray expression analysis followed by validation using RNA-seq and qRT-PCR experiments at the RNA levels and further validation at the DNA level by qPCR of target inserts for selected targets in eutopic endometrium samples obtained from control (CON) and stage IV ovarian endometriosis (OE), either from fertile (FCON and FOE; n = 30/each) or infertile (ICON and IOE; n = 30/each) women, were performed. Results Six coding (AMELY, PCDH11, SRY, TGIF2LY, TSPY3, and USP9Y) and 10 non-coding (TTTY2, TTTY4C, TTTY5, TTTYY6, TTTY8, TTTY10, TTTY14, TTTY21, TTTY22, and TTTY23) genes exhibited a bimodal pattern of expression characterised by low expression in samples from fertile patients and high expression in samples from infertile patients. Seven coding MSY-linked genes (BAGE, CD24, EIF1AY, NLGN4Y, PRKY, VCY and ZFY) exhibited differential regulation in microarray analysis, and this change was validated by RNA-seq or qRT-PCR. DNA inserts for 7 genes in various samples were validated by qPCR. The prevalence and concentration of PCR-positive target inserts for BAGE, PRKY, TTTY9A and ZFY displayed higher values in the fertile, control (FCON) patients compared with the fertile, endometriosis patients (FOE). Conclusion Several coding and non-coding MSY-linked genes displayed microchimerism as evidenced by the presence of their respective DNA inserts, along with their differential transcript expression, in the endometrium during endometriosis and in cases of infertility. Electronic supplementary material The online version of this article (10.1186/s12958-019-0465-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Bhat

Sharma

Roy

et al. 2019

Reprod Biol Endocrinol

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“…With advancements in global transcriptome profiling technique, the deregulation of lncRNAs has recently been demonstrated to be related to various human diseases, including most notably cancers [ 1 , 2 ], neurological disorders [ 3 ], and cardiovascular diseases [ 4 , 5 ]. Increasingly, some lncRNAs play a crucial role in cancer progression, such as proliferation, invasion and metastasis [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA MIAT in development and disease: a new player in an old game

Sun¹,

Huang²,

Li³

et al. 2018

J Biomed Sci

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BackgroundLong non-coding RNAs (lncRNAs), which are a portion of non-protein-coding RNAs (ncRNAs), have manifested a paramount role in the pathophysiology of human diseases, particularly in pathogenesis and progression of disease.Main body of the abstractMyocardial infarction associated transcript (MIAT), which was recently found to demonstrate aberrant expression in various diseases, such as myocardial infarction, schizophrenia, ischemic stroke, diabetic complications, age-related cataract and cancers, is a novel disease-related lncRNA. This work summarize current evidence regarding the biological functions and underlying mechanisms of lncRNA MIAT during disease development.Short conclusionLncRNA MIAT likely represents a feasible cancer biomarker or therapeutic target.

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“…Another important lncRNA, lnc-KDM5D-4, with a regulatory effect on lipin2, that in turn, lead to increased lipid droplet formation in HepG2 cells [ 30 ].…”

Section: Central Nodes To Control Fatty Acids Imbalance In Cancermentioning

confidence: 99%

Non-Coding and Regulatory RNAs as Epigenetic Remodelers of Fatty Acid Homeostasis in Cancer

Cruz-Gil

Fernández

Martínez

et al. 2020

Cancers

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Cancer cells commonly display metabolic fluctuations. Together with the Warburg effect and the increased glutaminolysis, alterations in lipid metabolism homeostasis have been recognized as a hallmark of cancer. Highly proliferative cancer cells upregulate de novo synthesis of fatty acids (FAs) which are required to support tumor progression by exerting multiple roles including structural cell membrane composition, regulators of the intracellular redox homeostasis, ATP synthesis, intracellular cell signaling molecules, and extracellular mediators of the tumor microenvironment. Epigenetic modifications have been shown to play a crucial role in human development, but also in the initiation and progression of complex diseases. The study of epigenetic processes could help to design new integral strategies for the prevention and treatment of metabolic disorders including cancer. Herein, we first describe the main altered intracellular fatty acid processes to support cancer initiation and progression. Next, we focus on the most important regulatory and non-coding RNAs (small noncoding RNA—sncRNAs—long non-coding RNAs—lncRNAs—and other regulatory RNAs) which may target the altered fatty acids pathway in cancer.

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A Novel Y-Specific Long Non-Coding RNA Associated with Cellular Lipid Accumulation in HepG2 cells and Atherosclerosis-related Genes

Cited by 31 publications

References 44 publications

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Long non-coding RNA MIAT in development and disease: a new player in an old game

Non-Coding and Regulatory RNAs as Epigenetic Remodelers of Fatty Acid Homeostasis in Cancer

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