2013
DOI: 10.1371/journal.pone.0061801
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A Novel Zebrafish Xenotransplantation Model for Study of Glioma Stem Cell Invasion

Abstract: Invasion and metastasis of solid tumors are the major causes of death in cancer patients. Cancer stem cells (CSCs) constitute a small fraction of tumor cell population, but play a critical role in tumor invasion and metastasis. The xenograft of tumor cells in immunodeficient mice is one of commonly used in vivo models to study the invasion and metastasis of cancer cells. However, this model is time-consuming and labor intensive. Zebrafish (Danio rerio) and their transparent embryos are emerging as a promising … Show more

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Cited by 91 publications
(114 citation statements)
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“…18,25 Based on work by others, we maintained our xenografts at 33°C, which is lower than the standard 37°C for culturing glioblastoma cells, but higher than the typical 28°C for zebrafish maintenance. 6,15 This temperature caused no observable zebrafish toxicity, yet resulted in adequate human glioblastoma growth and migration/invasion over the 3-4 day assay period to detect exposure-dependent differences between treatment groups.…”
Section: Transplantation Conditions In Brain Support Glioblastoma Growthmentioning
confidence: 99%
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“…18,25 Based on work by others, we maintained our xenografts at 33°C, which is lower than the standard 37°C for culturing glioblastoma cells, but higher than the typical 28°C for zebrafish maintenance. 6,15 This temperature caused no observable zebrafish toxicity, yet resulted in adequate human glioblastoma growth and migration/invasion over the 3-4 day assay period to detect exposure-dependent differences between treatment groups.…”
Section: Transplantation Conditions In Brain Support Glioblastoma Growthmentioning
confidence: 99%
“…Measurements of migration and invasion in embryonic zebrafish xenograft models has typically involved qualitative scoring or simply counting the number of cells to move away from the initial cell mass with no information on distance. 5,15,16 Our method provides a quantitative method to measure changes in the cells spread outward over concentric distances from the center of the cancer mass. This approach is similar to Zhang et al who measured chemical inhibition of leukemia stem cell migration in a zebrafish xenograft model.…”
Section: Embryo-larval Zebrafish Xenograft Assay 325mentioning
confidence: 99%
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