A novel β-1,3-glucanase Gns6 from rice possesses antifungal activity against Magnaporthe oryzae

Wang, Yanxin; Liu, Muxing; Wang, Xiaowen; Zhong, Lingli; Shi, Guolong; Xu, Ye; Li, Yangqing; Li, Ruolin; Huang, Yan; Ye, Xianfeng; Li, Zhoukun; Cui, Zhongli

doi:10.1016/j.jplph.2021.153493

Cited by 26 publications

(15 citation statements)

References 54 publications

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“…Besides cell wall organization, gene ontology (GO) classification of biological process of the DEGs were abundantly enriched in defense response ( Figure 4C ). And among those DEGs, we found that the transcripts of pathogenesis-related ( PR ) gene, chitinase gene, Gns6 ( Wang et al., 2021 ), OsMBL1 ( Han et al., 2019 ), and Xa21 ( Song et al., 1995 ), were highly upregulated in dnl19 mutant ( Figure 4D ). The reported narrow leaf and dwarf1 ( nd1 ) was a OsCSLD4 functional disruption mutant in Zhongxian 3037 indica variety ( Li et al., 2009 ).…”

Section: Resultsmentioning

confidence: 99%

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Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

et al. 2022

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Plant cell wall is a complex and changeable structure, which is very important for plant growth and development. It is clear that cell wall polysaccharide synthases have critical functions in rice growth and abiotic stress, yet their role in plant response to pathogen invasion is poorly understood. Here, we describe a dwarf and narrowed leaf in Hejiang 19 (dnl19) mutant in rice, which shows multiple growth defects such as reduced plant height, enlarged lamina joint angle, curled leaf morphology, and a decrease in panicle length and seed setting. MutMap analysis, genetic complementation and gene knockout mutant show that cellulose synthase-like D4 (OsCSLD4) is the causal gene for DNL19. Loss function of OsCSLD4 leads to a constitutive activation of defense response in rice. After inoculation with rice blast and bacterial blight, dnl19 displays an enhanced disease resistance. Widely targeted metabolomics analysis reveals that disruption of OsCSLD4 in dnl19 resulted in significant increase of L-valine, L-asparagine, L-histidine, L-alanine, gentisic acid, but significant decrease of L-aspartic acid, malic acid, 6-phosphogluconic acid, glucose 6-phosphate, galactose 1-phosphate, gluconic acid, D-aspartic acid. Collectively, our data reveals the importance of OsCSLD4 in balancing the trade-off between rice growth and defense.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

et al. 2022

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“…Two enzymes associated with fungal proliferation were studied: chitinase B, which is responsible for the regulation of chitin in the fungus, and 1,3-β-glucanase [38]. Both enzymes are associated with the hydrolysis of 1,3-and 1,6-β-glucan (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic Regulation; An in <i>Silico</i> Approach

Carrasco-Carballo¹,

Marín-Merino²,

Merino-Montiel³

et al. 2021

AER

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“…31 OsGns6 can be induced by rice blast fungus, and in vitro experiments have demonstrated that the Gns6 protein can inhibit germ tube development and appressorium formation in rice blast fungus. 32 Previous studies have also reported that overexpression of ⊎-1,3-glucanase genes derived from alfalfa and tobacco can confer resistance to sheath blight in transgenic rice. 33,34 ⊎-1,6-Glucan is an essential component of fungal cell wall and can be cross-linked with ⊎-1,3-glucan, chitin and the glycophosphatidylinositol anchor to maintain cell stability.…”

Section: Introductionmentioning

confidence: 99%

“…Overexpression of OsGns1 in rice can improve the resistance of plants to rice blast 31 . OsGns6 can be induced by rice blast fungus, and in vitro experiments have demonstrated that the Gns6 protein can inhibit germ tube development and appressorium formation in rice blast fungus 32 . Previous studies have also reported that overexpression of β‐1,3‐glucanase genes derived from alfalfa and tobacco can confer resistance to sheath blight in transgenic rice 33,34 …”

Section: Introductionmentioning

confidence: 99%

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

Shen

Wang

et al. 2023

Pest Management Science

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BACKGROUND: Frequent fungal diseases tend to lead to severe losses in rice production. As a main component of the fungal cell wall, glucan plays an important role in the growth and development of fungi. Glucanase can inhibit the growth of fungi by breaking glycosidic bonds, and may be a promising target for developing rice varieties with broad-spectrum disease resistance.RESULTS: We transferred a codon-optimized ⊎-1,6-glucanase gene (GluM) from myxobacteria into the japonica rice variety Zhonghua11 (ZH11), and obtained a large number of individual transgenic plants with GluM overexpression. Based on molecular analysis, three single-copy homozygous lines with GluM overexpression were selected for assessment of fungal disease resistance at the T 3 generation. Compared with that of the recipient cultivar ZH11, the area of rice blast lesion in transgenic rice was reduced by 82.71%; that of sheath blight lesion was decreased by 35.76%-43.67%; the sheath blight resistance in the field was enhanced by an average of 0.75 grade over 3 years; and the incidence of diseased panicles due to rice false smut was decreased by 65.79%. More importantly, there was no obvious loss of yield (without a significant effect on agronomic traits). Furthermore, plants overexpressing a ⊎-1,6-glucanase gene showed higher disease resistance than rice plants overexpressing a ⊎-1,3-glucanase gene derived from tobacco.CONCLUSION: The ⊎-1,6-glucanase gene GluM can confer broad-spectrum disease resistance to rice, providing an environmentally friendly alternative way to effectively manage fungal pathogens in rice production.

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A novel β-1,3-glucanase Gns6 from rice possesses antifungal activity against Magnaporthe oryzae

Cited by 26 publications

References 54 publications

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic Regulation; An in <i>Silico</i> Approach

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

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A novel β-1,3-glucanase Gns6 from rice possesses antifungal activity against Magnaporthe oryzae

Cited by 26 publications

References 54 publications

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Rice cellulose synthase-like protein OsCSLD4 coordinates the trade-off between plant growth and defense

Steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic Regulation; An in &lt;i&gt;Silico&lt;/i&gt; Approach

Overexpression of a beta‐1,6‐glucanase gene GluM in transgenic rice confers high resistance to rice blast, sheath blight and false smut

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Steroidal Plant Growth Promoters vs. Phytopathogens, via Enzymatic Regulation; An in <i>Silico</i> Approach