The properties and spinning limits of carded cotton vortex spun yarns were investigated through the progressive change in yarn count. The yarns were tested for structural and physical properties. Critical parameters were obtained, including the ratio of wrapper fibers to core fibers, proportion and average length of different structural classes in the yarn. The structural analysis revealed that the wrapper fibers constitute an increasing proportion of the fibers as the yarn becomes finer. Moreover, the proportion and average length of different structural classes in vortex spun yarns change with yarn count. The analysis of the data obtained from yarn testing showed that as the yarn becomes finer, the yarn becomes more uneven and the number of yarn imperfections markedly increase. On the other hand, the tensile properties of vortex spun yarns remain almost unchanged as the yarn gets finer.