A Numerical Study of the Influence of Cellular Adhesion on Prestress in Atomic Force Microscopy Measurements

Sraj, Ihab; Chan, Kit Yan; Κωνσταντόπουλος, Κωνσταντίνος; Eggleton, Charles D.

doi:10.1166/jamr.2011.1060

Cited by 1 publication

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“…The IBM is a cell-fluid interaction solver that has been used extensively to simulate biological systems such as cell adhesion [25], cell adhesion in atomic force microscopy measurements [26] and red blood cell motion through microvascular bifurcation [27]. IBM splits the numerical solution onto two grids: a stationary grid that has a fixed position with time representing the three-dimensional fluid domain and a moving grid representing the two-dimensional immersed boundary.…”

Section: Immersed Boundary Methodsmentioning

confidence: 99%

Numerical model for the deformation of nucleated cells by optical stretchers

Sraj¹,

Francois²,

Marr³

et al. 2015

J. Opt.

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In this paper, we seek to model the deformation of nucleated cells by single diode-laser bar optical stretchers. We employ a recently developed computational model, the Dynamic Ray-Tracing method, to determine the stress distribution induced by the applied optical forces on a capsule encapsulating a nucleus of different optical properties. These forces are shape dependent and can deform real non-rigid objects; thus resulting in a dynamically changing optical stress distribution with cell and nucleus deformation. Chinese hamster ovary cell is a common biological cell that is of interest to the biomedical community because of their use in recombinant protein therapeutics and is an example of a nucleated cell. To this end, we model chinese hamster ovary cells as two three-dimensional elastic capsules of variable inner capsule size immersed in a fluid where the hydrodynamic forces are calculated using the Immersed Boundary Method. Our results show that the presence of a nucleus has a major effect on the force distribution on the cell surface and the net deformation. Scattering and gradient forces are reported for different nucleus sizes and the effect of nucleus size on the cell deformation is discussed.

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Section: Immersed Boundary Methodsmentioning

confidence: 99%