A nutrient medium for development of cell dense inoculum in mixotrophic mode to seed mass culture units of Dunaliella salina

Keerthi, Suman; Koduru, Uma Devi; Sarma, Nittala S.

doi:10.1127/algol_stud/2014/0177

Cited by 2 publications

(3 citation statements)

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“…Due to the heterogeneity of both the salinities and glycerol levels, and the cell growth reported from previous studies [14,20,28], the effect of glycerol concentration on the culture of D. salina under better growth isosmotic conditions (1.5 M NaCl) [29] was evaluated. This saline condition and 12.5 mM glycerol promoted the best growth of this microalga (1.19 × 10 6 cells mL −1 ) (p < 0.05), even exceeding cell density obtained in photoautotrophic cultures (IM) (Figure 1).…”

Section: Effect Of Glycerol Concentration On Mixotrophic Culture Of D Salina Under 15 M Nacl Isosmotic Salinitymentioning

confidence: 99%

“…A Hanna meter (HI98130) recorded the influence of pH on the cell growth in glycerol. The microalgal cultures were gently hand-shaken (two times daily for 2 min) [14], and CO 2 unfed. The mixotrophic growth of D. salina was compared only to the photoautotrophic control, because no cellular growth on glycerol was observed under heterotrophic conditions without light.…”

Section: Mixotrophic Growth Conditions Set Upmentioning

confidence: 99%

“…Efforts to develop mixotrophic growth media for D. salina and many other microalgae are currently underway. The growth of D. salina on glucose [10][11][12] and acetate [10,13,14] supplemented media was reported, but they still exhibit predominantly low growth rates or a very low amount of β-carotene. Regarding substrates, glucose has the disadvantage of reducing photochemical O 2 evolution, cellular affinity for CO 2 , and the synthesis of the Calvin cycle enzymes in microalgae [15].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

2021

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Current mixotrophic culture systems for Dunaliella salina have technical limitations to achieve high growth and productivity. The purpose of this study was to optimize the mixotrophic conditions imposed by glycerol, light, and salinity that lead to the highest biomass and β-carotene yields in D. salina. The combination of 12.5 mM glycerol, 3.0 M salinity, and 50 μmol photons m−2 s−1 light intensity enabled significant assimilation of glycerol by D. salina and consequently enhanced growth (2.1 × 106 cell mL−1) and β-carotene accumulation (4.43 pg cell−1). The saline and light shock induced the assimilation of glycerol by this microalga. At last stage of growth, the increase in light intensity (300 μmol photons m−2 s−1) caused the β-carotene to reach values higher than 30 pg cell−1 and tripled the β-carotene values obtained from photoautotrophic cultures using the same light intensity. Increasing the salt concentration from 1.5 to 3.0 M NaCl (non-isosmotic salinity) produced higher growth and microalgal β-carotene than the isosmotic salinity 3.0 M NaCl. The mixotrophic strategy developed in this work is evidenced in the metabolic capability of D. salina to use both photosynthesis and organic carbon, viz., glycerol that leads to higher biomass and β-carotene productivity than that of an either phototrophic or heterotrophic process alone. The findings provide insights into the key role of exogenous glycerol with a strategic combination of salinity and light, which evidenced unknown roles of this polyol other than that in osmoregulation, mainly on the growth, pigment accumulation, and carotenogenesis of D. salina.

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Section: Effect Of Glycerol Concentration On Mixotrophic Culture Of D Salina Under 15 M Nacl Isosmotic Salinitymentioning

confidence: 99%

Section: Mixotrophic Growth Conditions Set Upmentioning

confidence: 99%