A one-step biuret assay for protein in the presence of detergent

Watters, Christopher

doi:10.1016/0003-2697(78)90475-x

Cited by 101 publications

(37 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For spectroscopic determinations, HPLCfractions were concentrated by ultrafiltration with an Amicon PM 30 membrane. Membrane protein was determined by a modified Biuret reaction [7], and solubilized membrane protein by the Lowry method in the presence of SDS [8]. Polyacrylamide gel electrophoresis was carried out by the Laemmli procedure 191 on 15% gels with an acrylamide to bisacrylamide ratio of 100: 1, and proteins were visualized by Coomassie staining.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Anemüller

Schäfer

1989

FEBS Letters

View full text Add to dashboard Cite

Cytochrome aa, serves as a terminal oxidase in the thermoacidophilic archaebacterium Sulfolobus acidocafdarius. A procedure for its isolation is described. The purified preparation consists of only one major polypeptide of 38 kDa apparent molecular mass. The enzyme contains two heme a molecules with midpoint potentials of + 220 and + 370 mV, respectively. The copper content is at least 2 Cu/q. It has only negligible capacity to oxidize cytochrome c, but rather serves as an oxidase for reduced caldariella quinone as present in the membrane of Sulfolobus.

show abstract

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Anemüller

Schäfer

1989

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…by computerized image analysis. Also, diaphragmatic myofibrillar pro-This disproportionate decrease in Type II fiber CSA resulted in tein concentration and total content were measured (16,17) along with an increase (p < 0.05) in the percentage of total area occupied diaphragmatic water content.…”

Section: Tissue Analysismentioning

confidence: 99%

Mechanical Ventilation–induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity

Shanely

Zergeroğlu

Lennon

et al. 2002

Am J Respir Crit Care Med

298

344

View full text Add to dashboard Cite

Prolonged mechanical ventilation (MV) results in reduced diaphrag-matic maximal force production and diaphragmatic atrophy. To investigate the mechanisms responsible for MV-induced diaphrag-matic atrophy, we tested the hypothesis that controlled MV results in oxidation of diaphragmatic proteins and increased diaphrag-matic proteolysis due to elevated protease activity. Further, we postulated that MV would result in atrophy of all diaphragmatic muscle fiber types. Mechanically ventilated animals were anesthe-tized, tracheostomized, and ventilated with 21% O2 for 18 hours. MV resulted in a decrease (p � 0.05) in diaphragmatic myofibrillar protein and the cross-sectional area of all muscle fiber types (i.e., I, IIa, IId/x, and IIb). Further, MV promoted an increase (p � 0.05) in diaphragmatic protein degradation along with elevated (p � 0.05) calpain and 20S proteasome activity. Finally, MV was also associated with a rise (p � 0.05) in both protein oxidation and lipid peroxidation. These data support the hypothesis that MV is associated with atrophy of all diaphragmatic fiber types, increased diaphragmatic protease activity, and augmented diaphragmatic ox-idative stress.

show abstract

“…Pectoralis muscle protein content was estimated using the biuret procedure described by Watters (1978). Muscle samples were homogenized in 10 ml/g tissue of 100 mM potassium phosphate, 2 mM EDTA, pH 7.3 for 10 s at a setting of 50 on a Tekmar Tissuemizer.…”

Section: Protein Determinationsmentioning

confidence: 99%

Maintenance of the pectoralis muscle during hibernation in the big brown bat,Eptesicus fuscus

Yacoe

1983

J Comp Physiol B

View full text Add to dashboard Cite

Summary.The relationship between pectoralis muscle mass and body mass is examined throughout the annual body mass cycle in Eptesicusfuscus in order to evaluate muscle maintenance during hibernation. E. fuscus undergoes large fluctuations in body mass during the year due to pregnancy, parturition, prehibernation fattening, and hibernation (Table 1). Parallel changes occur in pectoralis muscle mass and total pectoralis protein mass (Table 2). The strong correlation between log pectoralis mass and log body mass ( Fig. 3) and the lack of correlation between pectoralis mass and forearm length ( Fig. 1, 2) suggest that the seasonal variation in pectoralis muscle mass represents a compensatory response to changing body mass. In active bats this relationship closely resembles the compensatory response predicted by flight theory.Both pectoralis muscle mass and body mass decrease significantly during four months of hibernation (Tables 1, 2). Although pectoralis mass and body mass are not significantly correlated after four months of hibernation the values fall within the range of observations in active summer bats (Fig. 3), indicating an approximate maintenance of the pectoralis mass/body mass relationship. The lack of correlation in hibernating bats may result from differences in the function of pectoralis muscle during activity (powering flight) and hibernation (thermogenesis and supply of gluconeogenic precursors). The significant elevation of pectoralis muscle protein concentration during prehibernation fattening and its progressive decrease during hibernation (Fig. 4) support the idea that the pectoralis muscle serves as an important source of gluconeogenic precursors during hibernation.

show abstract

A one-step biuret assay for protein in the presence of detergent

Cited by 101 publications

References 5 publications

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Mechanical Ventilation–induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity

Maintenance of the pectoralis muscle during hibernation in the big brown bat,Eptesicus fuscus

Contact Info

Product

Resources

About

A one-step biuret assay for protein in the presence of detergent

Cited by 101 publications

References 5 publications

Cytochrome aa3 from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Cytochrome aa3 from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Mechanical Ventilation–induced Diaphragmatic Atrophy Is Associated with Oxidative Injury and Increased Proteolytic Activity

Maintenance of the pectoralis muscle during hibernation in the big brown bat,Eptesicus fuscus

Contact Info

Product

Resources

About

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

Cytochrome aa₃ from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius