A one-tube rapid visual CRISPR assay for the field detection of Japanese encephalitis virus

Xu, Bingrong; Gong, Pan; Zhang, Yi; Wang, Yuan; Tao, Dagang; Fu, Lanting; Khazalwa, Emmanuel Mulaya; Liu, Hailong; Zhao, Shuhong; Zhang, Xuying; Xie, Shengsong

doi:10.1016/j.virusres.2022.198869

Cited by 17 publications

(10 citation statements)

References 50 publications

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“…75 A rapid visual CRISPR assay termed RAVI-CRISPR was developed to ensure accurate, sensitive, and convenient diagnosis of JEV. 76 RT-LAMP was used to amplify the JEV C gene, and five CRISPR RNAs (crRNAs) targeting the C gene were designed. Further experiments to assess sensitivity and validate the specificity of the platform were conducted using crRNA producing the highest fluorescence signal intensity.…”

Section: Other Viral Pathogensmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

Yigci,

Atçeken,

Yetisen

et al. 2023

ACS Omega

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Section: Other Viral Pathogensmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

Yigci,

Atçeken,

Yetisen

et al. 2023

ACS Omega

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“…A very recent 2022 work involved a one-step point-of-care RApid VIsual Clustered regularly interspaced short palindromic repeats (RAVI-CRISPR) assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and CRISPR/Cas12a targeting. The developed assay could rapidly detect JEV with an LOD of 8.97 copies of the C gene sequence of JEV RNA within approximately 60 min (Xu et al 2022 ).…”

Section: Biosensors For Jev Diagnosticsmentioning

confidence: 99%

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

Roberts

Gandhi

2022

Proc.Indian Natl. Sci. Acad.

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Advanced biosensor technology research is imperative for the management of infectious disease outbreaks such as Japanese Encephalitis (JE), a zoonotic disease caused by the flavivirus JE virus (JEV) which is transmitted to humans (dead-end hosts) from the amplification host, pigs, via mosquitoes. To avoid future pandemic scenarios, proactive research rather than responsive research in the field of diagnostics is a requirement for development of rapid, sensitive and specific screening detection methods. In this mini-review, we have critically compared and evaluated the different types of biomarkers (antigen, antibody, nucleic acid) identified for JEV diagnostics and their specific roles in the manifestation of the infection which may be potentially used for therapeutics and drug development as no treatment is available for JE. Furthermore, different biosensors developed for the detection of JEV biomarkers have been discussed in detail to give an overview of the working principles (electrochemical, optical, etc.), fabrication components (signal amplifier, bioreceptor, etc.), detection limits and response times. This review provides a compact compiled base on available JEV diagnostic research work being currently carried out along with their limitations, future prospective, and major challenges faced. This will enable future development of rapid point-of-care diagnostic screening methods for JEV infection management, which may help reduce number of fatalities.

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“…By Combining the DETECTR strategy [ 31 ] and the ROX-labeled reporter, RApid VIsual CRISPR (RAVI-CRISPR) has been established as a device-less (only a portable rechargeable incubator is required) single-tube colorimetric POC platform [ 61 ]. The RAVI-CRISPR has been successfully applied for detection of SARS-CoV-2 [ 61 ], ASFV [ 61 ], and JEV [ 86 ], for sex determination in pigs [ 101 ], as well as for recognition of meat species and meat products [ 104 ]. The RAVI-CRISPR in these applications has a LOD of 2–9 total copies and takes only 35–60 min using naked-eye colorimetric detection.…”

Section: Development Of Crispr/cas-based Nucleic Acid Detection Systemsmentioning

confidence: 99%

“…Besides, the strategy of combining isothermal amplification with the collateral cleavage activity of CRISPR/Cas12a (DETECTR [ 31 ]) or CRISPR/Cas13a (SHERLOCK [ 65 ]) has also been applied for the detection of PCV3 (ssDNA circovirus, ERA-CRISPR/Cas12a) [ 84 ], PPV (non-enveloped DNA virus, ERA-CRISPR/Cas12a) [ 85 ], EMCV (non-enveloped ssRNA virus, RAA-CRISPR/Cas13a) [ 87 ], JEV (ssRNA virus, RT-LAMP-CRISPR/Cas12a) [ 86 ], LSDV (linear dsDNA virus, RPA-Cas12a) [ 88 ], BVDV (positive-sense ssRNA virus, CRISPR-Cas13a-based) [ 89 ], and CaPV (LAMP-CRISPR/Cpf1) [ 90 ]. These diagnostic methods have robustness, convenience, sensitivity, specificity, affordability and potential adaptation for in-field detection or surveillance of the viruses in clinical and vector samples.…”

Section: Current Applications Of Crispr/cas-based Nucleic Acid Detect...mentioning

confidence: 99%

Development of CRISPR-Mediated Nucleic Acid Detection Technologies and Their Applications in the Livestock Industry

Zhang¹

2022

Genes

Self Cite

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The rapid rate of virus transmission and pathogen mutation and evolution highlight the necessity for innovative approaches to the diagnosis and prevention of infectious diseases. Traditional technologies for pathogen detection, mostly PCR-based, involve costly/advanced equipment and skilled personnel and are therefore not feasible in resource-limited areas. Over the years, many promising methods based on clustered regularly interspaced short palindromic repeats and the associated protein systems (CRISPR/Cas), i.e., orthologues of Cas9, Cas12, Cas13 and Cas14, have been reported for nucleic acid detection. CRISPR/Cas effectors can provide one-tube reaction systems, amplification-free strategies, simultaneous multiplex pathogen detection, visual colorimetric detection, and quantitative identification as alternatives to quantitative PCR (qPCR). This review summarizes the current development of CRISPR/Cas-mediated molecular diagnostics, as well as their design software and readout methods, highlighting technical improvements for integrating CRISPR/Cas technologies into on-site applications. It further highlights recent applications of CRISPR/Cas-based nucleic acid detection in livestock industry, including emerging infectious diseases, authenticity and composition of meat/milk products, as well as sex determination of early embryos.

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A one-tube rapid visual CRISPR assay for the field detection of Japanese encephalitis virus

Cited by 17 publications

References 50 publications

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

Loop-Mediated Isothermal Amplification-Integrated CRISPR Methods for Infectious Disease Diagnosis at Point of Care

A brief review on novel biomarkers identified and advanced biosensing technologies developed for rapid diagnosis of Japanese Encephalitis Virus

Development of CRISPR-Mediated Nucleic Acid Detection Technologies and Their Applications in the Livestock Industry

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