A palette of site-specific organelle fluorescent thermometers

Liu, Xiao; Yamazaki, Takeru; Kwon, Hyeokjin; Arai, Soichi; Chang, Young‐Tae

doi:10.1016/j.mtbio.2022.100405

Cited by 17 publications

(33 citation statements)

References 55 publications

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“…Moreover, Chang et al reported a uorescent temperature probe (29) based on an asymmetric BODIPY-based dye. 79 The asymmetric structure made the benzene ring rotate with a lower energy barrier, thereby increasing the probe's temperature-dependent rotational freedom. We can nd that most of the probes for mitochondrial temperature detection are based on rhodamine and BODIPY dyes.…”

Section: Intramolecular-rotation-based Temperature Probesmentioning

confidence: 99%

“…86 Thus, the temperature of the ER and lysosomes is critical for maintaining biological system homeostasis. Fluorescent temperature probes ( 36 , targeting lysosomes), 79 ( 37 , targeting ER), 79 and ( 38 , targeting ER) 87 have been used to achieve suborganelle temperature responses through fluorescence changes associated with rotation of the benzene ring. Additionally, Kuang et al (2014) reported a fluorescent temperature probe ( 39 ) based on BODIPY dyes for targeting lysosomes.…”

Section: Fluorescent Thermometry Of Other Organellesmentioning

confidence: 99%

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Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Li,

Zhang

et al. 2024

Anal. Methods

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Section: Intramolecular-rotation-based Temperature Probesmentioning

confidence: 99%

Section: Fluorescent Thermometry Of Other Organellesmentioning

confidence: 99%

Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Li,

Zhang

et al. 2024

Anal. Methods

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“…Such a study would provide a picture of how alterations in mitochondrial metabolism and heat production affect intracellular temperature and its regulation more globally. An alternative approach would be to employ the recently developed palette of smallmolecule intracellular temperature reporters, the Thermo Greens 35 . Earlier studies using temperature-sensitive dyes targeted specifically to the ER 36,37,38 have indicated how the temperature of this organelle responds to external stresses and physiological stimuli.…”

Section: Validation and Calibration Of High Mitochondrial Temperaturementioning

confidence: 99%

Mitochondrial temperature homeostasis resists external metabolic stresses

Terzioglu,

Veeroja,

Montonen

et al. 2023

Preprint

Self Cite

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Based on studies with a fluorescent reporter dye, Mito Thermo Yellow, and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one insect cell-line was estimated to be up to 15 °C above that of the external environment to which the cells were exposed. High mitochondrial temperature was maintained in the face of a variety of metabolic stresses, including substrate starvation or modification, decreased ATP demand due to inhibition of cytosolic protein synthesis, inhibition of the mitochondrial adenine nucleotide transporter and, if an auxiliary pathway for electron transfer was available via the alternative oxidase, even respiratory poisons acting downstream of complex I. We propose that the high temperature of active mitochondria is an inescapable consequence of the biochemistry of oxidative phosphorylation and is homeostatically maintained as a primary feature of mitochondrial metabolism.IMPACT STATEMENTMitochondria are at least 15 °C hotter than their external environment in living cells. In response to diverse metabolic stresses, mitochondrial temperature re-adjusts to this value whenever possible.

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“…[22,29] It is also effective for remote measurement of temperature in micro and nanostructured systems, [26,28,33] including in vitro and in vivo measurements. [13,[39][40][41][42][43] In that case, it is common to refer to the thermal readout technique as luminescence nanothermometry. The decisive advantage of possible remote and minimally invasive temperature measurements at those lateral dimensions is the reason for the growing popularity of the technique in recent years.…”

Section: Introductionmentioning

confidence: 99%

Spotlight on Luminescence Thermometry: Basics, Challenges, and Cutting‐Edge Applications

et al. 2023

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Luminescence (nano)thermometry is a remote sensing technique that relies on the temperature dependency of the luminescence features (e.g., bandshape, peak energy or intensity, and excited state lifetimes and risetimes) of a phosphor to measure temperature. This technique provides precise thermal readouts with superior spatial resolution in short acquisition times. Although luminescence thermometry is just starting to become a more mature subject, it exhibits enormous potential in several areas, e.g., optoelectronics, photonics, micro‐ and nanofluidics, and nanomedicine. This work reviews the latest trends in the field, including the establishment of a comprehensive theoretical background and standardized practices. The reliability, repeatability, and reproducibility of the technique are also discussed, along with the use of multiparametric analysis and artificial‐intelligence algorithms to enhance thermal readouts. In addition, examples are provided to underscore the challenges that luminescence thermometry faces, alongside the need for a continuous search and design of new materials, experimental techniques, and analysis procedures to improve the competitiveness, accessibility, and popularity of the technology

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A palette of site-specific organelle fluorescent thermometers

Cited by 17 publications

References 55 publications

Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Response strategies and biological applications of organic fluorescent thermometry: cell- and mitochondrion-level detection

Mitochondrial temperature homeostasis resists external metabolic stresses

Spotlight on Luminescence Thermometry: Basics, Challenges, and Cutting‐Edge Applications

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