A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos

Pruvot, Benoist; Quiroz, Yobhana; Voncken, Audrey; Jeanray, Nathalie; Piot, Amandine; Martial, Joseph; Müller, Marc

doi:10.1016/j.reprotox.2012.07.010

Cited by 42 publications

(38 citation statements)

References 56 publications

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“…Interestingly, another two psychotropic drugs, carbamazepine and valproic acid commonly used for the treatment of epilepsy, induced a significant and concentration-dependent decrease of the heartbeat rate in a laboratory test, 46 which was exactly opposite to the result of the present study. It was reported that when rats were exposed to gabapentin, the heartbeat rate was elevated too.…”

Section: 45contrasting

confidence: 93%

The assessment of the eco-toxicological effect of gabapentin on early development of zebrafish and its antioxidant system

Zhou

Qian

et al. 2018

RSC Adv.

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Gabapentin (GAB) is an emerging contaminant that is frequently detected in water bodies across the globe.The present study used zebrafish as a model organism to investigate the effects of GAB on the early development of zebrafish and on its antioxidant system. Acute toxicity tests indicated that the 96 h LC 50 value of GAB for zebrafish embryos was 59.9 g L À1 . Further, it was observed that GAB causes malformation of embryos such as hemagglutination and pericardial edema. Compared to the control group, a significant enhancement (p < 0.05) of heartbeat rates was found at GAB concentrations exceeding 50 mg L À1, while the swimming frequency was clearly increased upon exposure to GAB at a concentration of 100 mg L À1 (p < 0.05). Additionally, the development of the zebrafish embryo was also negatively impacted after exposure to GAB as demonstrated by significantly decreased body lengths. Exposure to GAB at concentrations exceeding 50 mg L À1 significantly influenced the development of zebrafish, leading to malformation of organs and abnormal movements. Although no significant developmental effects of GAB were observed at environmentally relevant concentrations (0.1 and 10 mg L À1), further research about the antioxidant system confirmed that severe oxidant injury happened inside the organisms. catalase (CAT), lactate dehydrogenase (LDH), glutathione S-transferase (GST), glutathione (GSH) and the ability of inhibition of hydroxyl radicals (IHR) were used as biomarkers in the present study to illustrate GAB toxicity at environmentally relevant concentrations. The resultsshowed that activities of CAT, LDH and GST as well as IHR were all elevated after GAB exposure, which proved that ROS were formed in the body as derived from GAB exposure. Among all of these biomarkers, CAT was the most sensitive one to evaluate the influence of GAB, and showed a significant increase even at a very low exposure concentration (0.1 mg L À1 ).

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Section: 45contrasting

confidence: 93%

The assessment of the eco-toxicological effect of gabapentin on early development of zebrafish and its antioxidant system

Zhou

Qian

et al. 2018

RSC Adv.

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“…This syndrome has been observed in zebrafish carrying mutations that disrupt the cardiovascular and other systems, as well as following exposure to a variety of cardiotoxic chemicals [65–67]. Based on our results with TCDD, a possible link between these factors and the syndrome of heart malformation and pericardial edema could be effects on epicardium formation.…”

Section: The Zebrafish Epicardium As a Sentinel For Other Cardiotosupporting

confidence: 58%

Epicardium Formation as a Sensor in Toxicology

Hofsteen¹,

Plavicki²,

Peterson³

et al. 2013

JDB

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Zebrafish (Danio rerio) are an excellent vertebrate model for studying heart development, regeneration and cardiotoxicity. Zebrafish embryos exposed during the temporal window of epicardium development to the aryl hydrocarbon receptor (AHR) agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exhibit severe heart malformations. TCDD exposure prevents both proepicardial organ (PE) and epicardium development. Exposure later in development, after the epicardium has formed, does not produce cardiac toxicity. It is not until the adult zebrafish heart is stimulated to regenerate does TCDD again cause detrimental effects. TCDD exposure prior to ventricular resection prevents cardiac regeneration. It is likely that TCDD-induced inhibition of epicardium development and cardiac regeneration occur via a common mechanism. Here, we describe experiments that focus on the epicardium as a target and sensor of zebrafish heart toxicity.

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“…VPA also induced cardiac malformation and pericardial edema in ZEBDET (Pruvot et al. ; Inoue et al. ).…”

Section: Heartmentioning

confidence: 99%

Using zebrafish in systems toxicology for developmental toxicity testing

Nishimura

Inoue

Sasagawa

et al. 2016

Congenital Anomalies

171

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With the high cost and the long-term assessment of developmental toxicity testing in mammals, the vertebrate zebrafish has become a useful alternative model organism for high-throughput developmental toxicity testing. Zebrafish is also very favorable for the 3R perspective in toxicology; however, the methodologies used by research groups vary greatly, posing considerable challenges to integrative analysis. In this review, we discuss zebrafish developmental toxicity testing, focusing on the methods of chemical exposure, the assessment of morphological abnormalities, housing conditions and their effects on the production of healthy embryos, and future directions. Zebrafish as a systems toxicology model has the potential to elucidate developmental toxicity pathways, and to provide a sound basis for human health risk assessments.

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A panel of biological tests reveals developmental effects of pharmaceutical pollutants on late stage zebrafish embryos

Cited by 42 publications

References 56 publications

The assessment of the eco-toxicological effect of gabapentin on early development of zebrafish and its antioxidant system

The assessment of the eco-toxicological effect of gabapentin on early development of zebrafish and its antioxidant system

Epicardium Formation as a Sensor in Toxicology

Using zebrafish in systems toxicology for developmental toxicity testing

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