A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic and immunological analysis

Lu, Chih‐Ming; Lin, Jainn-Jim; Huang, Han-Hsiang; Ko, Ying‐Chin; Hsu, Jue‐Liang; Chen, Jiing-Chuan; Din, Zhong-Hao; Wu, Yu-Jen

doi:10.1186/1471-2407-14-363

Cited by 21 publications

(13 citation statements)

References 33 publications

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“…Therefore, the results of the current study indicated that high Annexin A2 expression is associated with poor prognosis in patients with laryngeal cancer, suggesting that Annexin A2 may act as an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer. Measuring the expression of Annexin A2 as well as the expression of other Annexins, including Annexins A4 and A3, may accurately predict the prognosis of different types of cancer, including urothelial carcinoma and cervical cancer (11,25,26). The predictive efficiency of Annexin A2 combined with other Annexins in laryngeal carcinoma requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Annexin A2 is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer

Luo

Xie

et al. 2017

Exp Ther Med

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Abstract. Due to the lack of a definite diagnosis, a frequent recurrence rate and resistance to chemotherapy or radiotherapy, the clinical outcome for patients with advanced laryngeal cancer has not improved over the last decade. Annexin A2 is associated with the invasion and metastasis of cancer cells. In the present study, it was demonstrated using differential proteomics analysis that Annexin A2 is highly expressed in laryngeal carcinoma tissues and this was confirmed using immunohistochemistry, which demonstrated that the expression of Annexin A2 in laryngeal carcinoma tissues was significantly higher than in healthy adjacent tissue. In addition, its potential predictive value in the prognosis of patients with laryngeal carcinoma was evaluated. The results demonstrated that Annexin A2 expression was significantly associated with tumor size, lymph node metastasis, distant metastasis and clinical stage. In addition, higher Annexin A2 expression was associated with a poor prognosis of patients with laryngeal cancer. Thus, the results of the present study indicate that Annexin A2 expression is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer.

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Section: Discussionmentioning

confidence: 99%

Annexin A2 is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer

Luo

Xie

et al. 2017

Exp Ther Med

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“…Previous reports have shown that CRT is up-regulated in other cancers. Close correlation between CRT expression and metastasis was observed [ 6 , 19 , 20 ], suggesting CRT is probably a pivotal molecular signal for cancer growth and progression. Yoon et al demonstrated that CRT is highly expressed in HCC patients’ samples when compared to noncarcinomatous hepatic tissues.…”

Section: Discussionmentioning

confidence: 99%

Calreticulin down-regulation inhibits the cell growth, invasion and cell cycle progression of human hepatocellular carcinoma cells

Feng

Zhou

et al. 2015

Diagn Pathol

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BackgroundHepatocellular carcinoma (HCC) is one of the most frequent cancers in the world. Calreticulin(CRT) is aberrantly overexpressed in many human cancer cells. The function of CRT in HCC cells remains unclear. We attempted to investigate the effects and the underlying mechanisms of CRT down-regulation on HCC cell growth, apoptosis, cell cycle progression and invasion.MethodsTo investigate the function of CRT in HCC cells, small interfering RNA (siRNA) was used to knock down the expression of CRT in SMMC7721 and HepG2 HCC cells. CRT expression was examined by Western blot and immunofluorescence. Cell proliferation was detected by CCK-8 assay. Cell cycle and apoptosis were measured by the flow cytometry. The invasion capability was assessed by transwell assay. The phosphorylation level of Akt was evaluated by Western blot.ResultsCompared with human hepatic cells L02, CRT was apparently up-regulated in SMMC7721, HepG2 and Huh7 HCC cells. Down-regulation of CRT expression effectively inhibited HCC cell growth and invasion. CRT knockdown induced cell cycle arrest and the apoptosis in SMMC7721 and HepG2 cells. Furthermore, down-regulation of CRT expression significantly decreased the Akt phosphorylation.ConclusionsCRT was aberrantly over-expressed in HCC cell lines. CRT over-expression contributes greatly to HCC malignant behavior, likely via PI3K/Akt pathway. CRT could serve as a potential biomarker and therapeutic target for hepatocellular carcinoma.

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“…Lu et al identified 55 differential proteins among totally 1028 protein spots in the urine of 13 UTUC patients compared with the urine of 20 healthy control adults using two-dimensional gel electrophoresis. Three proteins (CALR, annexin A2 and annexin A3) were found to be essential secreting proteins in the urine from UTUC tumor tissues, suggesting their potential role as a panel of biomarkers [52]. However, the major limitations shared by these studies were their retrospective nature and small sample sizes.…”

Section: Potential Biomarkers From Gene Expression Profilingmentioning

confidence: 99%

Prognostic Genetic Signatures in Upper Tract Urothelial Carcinoma

Bagrodia

Eugene

2016

Curr Urol Rep

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Urothelial carcinoma is a highly heterogeneous disease that can arise throughout the entire urothelial lining from the renal pelvis to the proximal urethra. Upper tract urothelial carcinoma (UTUC) is rare and while it shares many similarities with urothelial carcinoma of bladder (UCB), there are also significant differences between UTUC and UCB regarding clinical management and outcomes. No major advances have been made recently in the development of new systemic therapies for urothelial carcinoma, partly due to the lack of understanding of underlying molecular pathogenetic mechanisms. In the past decade, the emergence of next-generation sequencing has greatly enabled genomic characterization of tumor samples. Researchers are currently exploring a personalized approach to augment traditional clinical decision-making based on genetic alterations. In the present review, we summarize current genomic advances in UTUC and discuss the potential implications of these developments for developing prognostic and predictive biomarkers.

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A panel of tumor markers, calreticulin, annexin A2, and annexin A3 in upper tract urothelial carcinoma identified by proteomic and immunological analysis

Cited by 21 publications

References 33 publications

Annexin A2 is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer

Annexin A2 is an independent prognostic biomarker for evaluating the malignant progression of laryngeal cancer

Calreticulin down-regulation inhibits the cell growth, invasion and cell cycle progression of human hepatocellular carcinoma cells

Prognostic Genetic Signatures in Upper Tract Urothelial Carcinoma

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